The design of anti-fouling and anti-hydrolysis cyclic peptides for accurate electrochemical antigen testing in human blood

Abstract

Antigen testing is an effective methodology for COVID-19 disease screening, and the receptor-binding domain of SARS-CoV-2 spike glycoprotein (RBD) is recognized as a key biomarker for the diagnosis, treatment, and...

Phospholipid Bilayer Integrated with Multifunctional Peptide for Ultralow-Fouling Electrochemical Detection of HER2 in Human Serum

“…The images in Figure S3A exhibit the morphology of SA-doped PEDOT showing a microporous structure, which was consistent with our previous work. 24 The presence of DSPE-PEG was clearly observed after DSPE-PEG modification (Figure S3B). These results verified the successful construction of the biosensor.…”

“…First of all, PEDOT/GCE was obtained by electropolymerization at a constant potential of 1.1 V for 20 s in a solution containing 3.0 mg mL −1 SA and 9.0 mM EDOT monomer. 24 Subsequently, the carboxyl group of SA in the PEDOT/GCE was activated for 0.5 h by EDC/ NHS solution (containing 0.4 M EDC and 0.1 M NHS), and then 20 μL of DSPE-PEG solution (10.0 mg mL −1 ) was dropped on the PEDOT/GCE surface and covered with a wet beaker for 2 h, and the DSPE-PEG (with amino group) was then attached to the modified electrode (with carboxyl group) via amide bonding to obtain the DSPE-PEG/PEDOT/GCE. Afterward, the prepared DSPE-PEG/PEDOT/GCE was soaked in 2.0 mM sulfo-SMCC aqueous solution for 1 h at room temperature.…”

“…In this work, an electrochemical biosensor for rapid and sensitive detection of HER2 was proposed by connecting a branched peptide (Pep) with integrated antifouling, antiprotease hydrolysis, and recognition functions to DSPE-PEG and sodium alginate (SA) doped poly(3,4-ethylenedioxythiophene) (PEDOT) 24 -modified electrodes (Scheme 1). The branched peptide consists of a zwitterionic peptide with antifouling property (ekekekekek) and a peptide aptamer with recognizing ability (refvffly).…”

Designed Polyhydroxyproline Helical Peptide with Ultrarobust Antifouling Capability for Electrochemical Sensing in Diverse Complex Biological Fluids

“…The significance of enhancing the resistance to protease degradation of peptide biosensors was thus appropriately emphasized. 21 Indeed, it is widely recognized that D-peptides possess excellent protease tolerance due to the fact that most proteases specifically recognize and hydrolyze peptides containing the natural L-amino acids. 6 Hence, by constructing the PHHP using exclusively D-type amino acids, inherent protease tolerance can be achieved.…”

Antifouling Electrochemiluminescence Biosensor Based on Bovine Serum Albumin Hydrogel for the Accurate Detection of p53 Gene in Human Serum

“…Furthermore, there is the challenge of nonspecific adsorption of contaminants, such as proteins, for the detection of biomarkers in complex biological samples, which can compromise the accuracy of the detection results. To mitigate this issue, researchers have explored a variety of antifouling materials, including hydrogels, polypeptides, 20 and zwitterionic polymers. 21 it difficult for nonspecific proteins to adhere, thus reducing interference on biosensors.…”