The Dependence of the Efficiency of Gene Transfer with the Use of Lipoplexes Based on New Dicationic Lipids on Their Structure

Жданов, Р. И.; El‐Kadi, Ayman O.S.; Московцев, А. А.; Sebyakin, Yu. L.; Ларин, А С; Muranov, A. V.; Хохлов, А. Р.

doi:10.1023/b:dobi.0000046632.50964.c7

Cited by 2 publications

(2 citation statements)

References 2 publications

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“…Pyridinium head groups have been made popular with the publication of the Saint series of lipids, though the pyridinium was bonded to a linker at C3 and an R group was attached to the nitrogen (40)(41)(42). Cationic lipids with a heterocyclic trimethylpyridinium as the polar head linked to two alkyl chains via a propyl group have shown good transfection in both in vitro and in vivo experiments (43). Unlike the Saint lipids, in the lipids described here, the pyridine is N-linked to the linker, i.e., glutamic acid.…”

Section: Resultsmentioning

confidence: 99%

Synthesis and in Vitro Evaluation of Glutamide-Containing Cationic Lipids for Gene Delivery

et al. 2006

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A novel series of cationic amphiphiles based on dialkyl glutamides with cationic pyridinium head group were synthesized as potential gene delivery agents. Four cationic lipids with glutamide as linker and varying chain lengths were tested for their transfection efficiency in three cell lines. The DNA-lipid complexes were characterized for their ability to bind to DNA, protection from nuclease digestion, size, zeta-potential, and toxicity. All four lipids demonstrated efficient transfection in MCF-7, COS, and HeLa cells, and the reporter gene expression was much higher with DOPE as the helper lipid in the formulation when compared to cholesterol. Among these 14-carbon lipids, lipid 2 has shown the highest transfection efficiency, complete protection of DNA from nuclease digestion, and low toxicity. Interestingly, lipid 2 has also shown remarkable enhancement in transfection in the presence of serum.

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Section: Resultsmentioning

confidence: 99%

Synthesis and in Vitro Evaluation of Glutamide-Containing Cationic Lipids for Gene Delivery

et al. 2006

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“…1,2 In order to expand the library of cationic amphiphiles, reveal the relationship between the structure and activity of this class of compounds and develop medical transport systems on their basis, we have suggested new modifications (1-3) of the structures reported previously.…”

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confidence: 99%

Self-organising aggregates of lipodipeptides in an aqueous medium and their complexes with DNA

Sebyakin

Буданова

Gur’eva

2007

Mendeleev Communications

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The Dependence of the Efficiency of Gene Transfer with the Use of Lipoplexes Based on New Dicationic Lipids on Their Structure

Cited by 2 publications

References 2 publications

Synthesis and in Vitro Evaluation of Glutamide-Containing Cationic Lipids for Gene Delivery

Synthesis and in Vitro Evaluation of Glutamide-Containing Cationic Lipids for Gene Delivery

Self-organising aggregates of lipodipeptides in an aqueous medium and their complexes with DNA

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