The Dependence of Dose-Effects in Human Radiation Cytogenetics

Djomina, E. A.

doi:10.33145/2304-8336-2019-24-235-249

Cited by 7 publications

(2 citation statements)

References 5 publications

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“…This means that the energy, which must be absorbed in the target to produce a biological effect, can result from either one or two hits. Therefore, the dose curves are approximated by the equation Y=αD+βD 2 +c, and the coefficients before D and D 2 reflect the relative role of the two mechanisms [22].…”

Section: Research Resultsmentioning

confidence: 99%

Biological features of blood lymphocytes of the primary patients with endometrial cancer

Domina

Hrinchenko

2021

SR: BS

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The aim: to examine the radiosensitivity of chromosomes of T-lymphocytes in the blood of primary patients with endometrial cancer depending on the radiation dose. It was expected that the investigations would reveal a cytogenetic parameter as a predictor of radiosensitivity in non-malignant cells of patients exposed to curative irradiation. Materials and methods. Blood samples from 20 primary patients and 30 conditionally healthy donors were examined. Peripheral blood T-lymphocytes culture test system with metaphase chromosome aberration analysis was used. X-ray test-irradiation was performed at G0-stage of the cell cycle in the dose range of 0.5–3.0 Gy. Results. It was shown that the spontaneous level of chromosome aberrations in lymphocytes of primary patients before anti-tumour therapy is 7,82±0,33 aberrations/100 metaphases. This is more than 2-fold higher than the upper limit of average population index and approximately 6-fold higher than the data of own control. In our study during X-ray irradiation of cells cultures of patients, it was found for the first time that the total frequency of radiation-induced chromosome aberrations obeys the classical linear quadratic dose dependence with a predominance of linear component values; the frequency of radiation markers – also linear quadratic dose dependence, but with a predominance of quadratic component. Conclusions. High specificity of T-lymphocyte chromosomes to exposure to ionizing radiation as well as strict dependence of chromosome aberration yield on exposure dose justify their use as predictors of radiosensitivity of healthy cells from the tumour environment. The revealed dependences of induction of chromosomal damage in T-lymphocytes of patients with endometrial cancer prove the need for a personalized approach to plan the course of radiation therapy

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Section: Research Resultsmentioning

confidence: 99%

Biological features of blood lymphocytes of the primary patients with endometrial cancer

Domina

Hrinchenko

2021

SR: BS

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“…1). Спостерігали радіаційно-індукований цитогенетичний ефект, характерний для дії іонізуючого випромінювання в культурі ЛПК людини на G 0 стадії мітотичного циклу [12], а саме -перевищення спонтанної частоти асиметричних і симетричних обмінних аберацій, які є класичними цитогенетичними маркерами радіаційного впливу (дицентричні та кільцеві хромосоми -1,57 та 0,48 на 100 метафаз відповідно; аномальні моноцентрики, які формуться внаслідок транслокацій та інверсій -1,08 на 100 метафаз). Також в опромінених лімфоцитах крові хворих на ХЛЛ зареєстрували зростання частоти простих аберацій хроматидного та хромосомного типів (одиночних ацентричних фрагментів -5,66 на 100 метафаз, вільних парних фрагментів -4,09 на 100 метафаз), які вважаються цитогенетичними маркерами хромосомної нестабільності.…”

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Realization of the rescue effect at the cytogenetic level due to the interaction between intact normal and irradiated malignant human blood lymphocytes

Shemetun¹,

Talan²,

Dibska³

et al. 2020

Dopov. Nac. akad. nauk Ukr.

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The effect of intact blood lymphocytes from conditionally healthy persons on the genome stability in blood lymphocytes of patients with primary diagnosis of B-cell chronic lymphocytic leukemia (CLL) exposed in vitro to γ-quanta 137Cs at the G0 stage of the cell cycle in a dose of 0.5 Gy was investigated. For the study, the own model system of co-cultivation of blood lymphocytes from persons with different genders that permit to study va rious manifestations of the bystander response phenomenon at the cytogenetic level was used. The decrease in the radiation-induced total frequency of chromosome aberrations in irradiated cells of patients with CLL (from 12.88 till 9.56 per 100 metaphases, p < 0.01) due to a reduction in the level of chromatid-type aberrations (from 5.35 till 2.83 per 100 cells, p < 0.001), which are considered as markers of the chromosomal instability, was established. The frequencies of unstable cytogenetic markers of a radiation exposure (dicentric and ring chromosomes) remained unchanged (p> 0.05). The obtained data indicate that the result of the interaction between irradiated in vitro blood cells of patients with B-cell CLL (target cells) with intact blood lymphocytes of relatively healthy individuals (bystander cells) is a decrease of the chromosome instability in target cells, which is similar to the radiation-induced rescue effect type one.

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Modern View of the Problem of Radiation Carcinogenesis

Domina

2023

Oncology

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Summary. Genetic and epigenetic determinants of radiation carcinogenesis are presented. Unlike genetic ones, changes arising from epigenetic modifications are potentially reversible. An increase in the power and dose of ionizing radiation leads to a decrease in the promoter and an increase in the initiating function of radiation in the development of radiation carcinogenesis. The problem of radiation carcinogenesis consists in understanding the mechanisms of action of radiation in small doses on the human genome. Small doses of absorbed radiation are statistically significant factors of increased risk of cancer. Preservation of the dose-effect relationship for radiochromosomal markers in the blood of liquidators with oncological pathology in the long term after the Chernobyl accident confirms the radiogenic nature of these diseases. Processes of various types of repair that eliminate radiation-induced DNA damage are discussed. The formation of the “bystander effect”, which is an important mechanism of radiation-induced instability of the genome, increases the carcinogenic risk in the area of action of small doses of ionizing radiation.

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The Dependence of Dose-Effects in Human Radiation Cytogenetics

Cited by 7 publications

References 5 publications

Biological features of blood lymphocytes of the primary patients with endometrial cancer

Biological features of blood lymphocytes of the primary patients with endometrial cancer

Realization of the rescue effect at the cytogenetic level due to the interaction between intact normal and irradiated malignant human blood lymphocytes

Modern View of the Problem of Radiation Carcinogenesis

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