Fluorescein has been used as a tracer in aerosol studies for the detection and quantification of aerosols. This report summarizes a sequence of experiments conducted to confirm our procedure used for the recovery and detection of fluorescein from filters. Our results confirmed the following: (1) The optimum excitation and emission wavelengths for fluorescein are 492 and 516 nm, respectively. (2) For fluorescence intensity (FI) measurements to be accurate, FI of a sample has to be significantly higher than the FI of water filled test tubes. (3) At higher gain settings, changes in the test tube orientation can affect the FI measurement of low fluorescence material. (4) Consistent alignment of the test tube in the fluorometer reduces the variation in FI measurements. (5) Below the self-quenching limit, the fluorometer readings increase linearly with increasing fluorescein concentrations. (6) After the pH of the recovery solution has reached 8, there is no significant effect on FI measurements for further increase in pH. (7) The change in pH does not affect the shape or the position of the peak of the emission spectrum. (8) Our procedure fully recovers fluorescein from glass fiber filters. (9) Fluorescein samples, prepared for fluorometer measurements, were stable over at least a 12 day period.