The demonstration of thiol groups in certain tissues by means of a new colored sulfhydryl reagent

Electron Microscopy of Retinal Photoreceptors

“…Sj5strand (25) postulated that the existence of dense bands in the outer segments is due to the osmium deposition at the level of protein layers. This interpretation is consistent with the fact that the protein of the visual cells outer segments has a high content of sulfhydryl groups (4,23,35) which are known to be very reactive with OsO4 (1). However, recent work in pure lipid models (30) has resulted in the detection of layered structures in phospholipid myelin figures; the dense bands observed seem to be a product of the reaction of OsO4 with double bonds in the fatty acid chains.…”

Conjugation of glutathione with the reactive metabolites of 1,1-dichloroethylene in murine lung and liver

“…To visualize the cellular location of GSH, histochemical staining was performed with Mercury Orange, an azo dye that reacts with tissue sulfhydryls, and the formation of GSH-Mercury Orange complexes was identified by fluorescence microscopy (Forkert and Moussa, 1989). The specificity of staining is based on the more rapid rate of reaction with nonprotein sulfhydryls or GSH than with protein -SH groups (Asghar et al, 1975;Bennet, 1951). Histochemical staining of tissue sections showed that GSH was highly localized in the Clara and alveolar septal cells in the lungs of untreated mice (Fig.…”

Microstructure and chemical composition of fossil mammalian teeth

“…Mercurials react with SH groups in intact proteins [16,[18][19][20][21][22]. Thus the reaction of the fossil processes with mercurochrome indicates the presence of SH groups.…”