The Demand for New Antibiotics: Antimicrobial Peptides, Nanoparticles, and Combinatorial Therapies as Future Strategies in Antibacterial Agent Design

León-Buitimea, Ángel; Garza-Cárdenas, César Rodolfo; Garza-Cervantes, Javier Alberto; Lerma-Escalera, Jordy Alexis; Morones‐Ramírez, José Rubén

doi:10.3389/fmicb.2020.01669

Cited by 197 publications

(167 citation statements)

References 96 publications

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“…A century and a half after the discovery of AMPs, out of more than 3000 characterized peptides, seven (oritavancin, gramicidin, daptomycin, colistin, vancomycin, dalbavancin, telavancin) are applicable to clinical practice [ 4 ]. Recently, the problems of using AMPs in veterinary medicine and medicine have been widely discussed, which stimulates studies of targeted delivery of AMPs (for example, in the form of metal nanoparticles) or the development of species-specific AMPs [ 33 , 34 , 35 ]. Undoubtedly, further research will continue the existing directions.…”

Section: Brief History Of Research Physicochemical Properties CLmentioning

confidence: 99%

Antimicrobial and Amyloidogenic Activity of Peptides. Can Antimicrobial Peptides Be Used against SARS-CoV-2?

Kurpe

Grishin

Surin

et al. 2020

IJMS

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At present, much attention is paid to the use of antimicrobial peptides (AMPs) of natural and artificial origin to combat pathogens. AMPs have several points that determine their biological activity. We analyzed the structural properties of AMPs, as well as described their mechanism of action and impact on pathogenic bacteria and viruses. Recently published data on the development of new AMP drugs based on a combination of molecular design and genetic engineering approaches are presented. In this article, we have focused on information on the amyloidogenic properties of AMP. This review examines AMP development strategies from the perspective of the current high prevalence of antibiotic-resistant bacteria, and the potential prospects and challenges of using AMPs against infection caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).

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Section: Brief History Of Research Physicochemical Properties CLmentioning

confidence: 99%

Antimicrobial and Amyloidogenic Activity of Peptides. Can Antimicrobial Peptides Be Used against SARS-CoV-2?

Kurpe

Grishin

Surin

et al. 2020

IJMS

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“…Importantly, Au NPs may be applied as biosensors or vessels for drug delivery and even selective treatment of cancer [ 27 , 28 , 29 ]. According to the literature, Au NPs have been reported as effectual antibacterial agents against antibiotic-resistant strains, such as S. aureus, Enterococcus faecium, Enterococcus faecalis, Escherichia coli, Salmonella typhimurium, Shigella dysenteriae , Vibrio cholerae and Mycobacterium tuberculosis [ 21 , 30 ]. It should be pointed out that Au NPs have been hallmarked as a very attractive alternative in developing new methods of infectious disease treatment [ 27 , 30 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

Bactericidal Properties of Rod-, Peanut-, and Star-Shaped Gold Nanoparticles Coated with Ceragenin CSA-131 against Multidrug-Resistant Bacterial Strains

et al. 2021

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Background: The ever-growing number of infections caused by multidrug-resistant (MDR) bacterial strains requires an increased effort to develop new antibiotics. Herein, we demonstrate that a new class of gold nanoparticles (Au NPs), defined by shape and conjugated with ceragenin CSA-131 (cationic steroid antimicrobial), display strong bactericidal activity against intractable superbugs. Methods: For the purpose of research, we developed nanosystems with rod- (AuR NPs@CSA-131), peanut-(AuP NPs@CSA-131) and star-shaped (AuS NPs@CSA-131) metal cores. Those nanosystems were evaluated against bacterial strains representing various groups of MDR (multidrug-resistant) Gram-positive (MRSA, MRSE, and MLSb) and Gram-negative (ESBL, AmpC, and CR) pathogens. Assessment of MICs (minimum inhibitory concentrations)/MBCs (minimum bactericidal concentrations) and killing assays were performed as a measure of their antibacterial activity. In addition to a comprehensive analysis of bacterial responses involving the generation of ROS (reactive oxygen species), plasma membrane permeabilization and depolarization, as well as the release of protein content, were performed to investigate the molecular mechanisms of action of the nanosystems. Finally, their hemocompatibility was assessed by a hemolysis assay. Results: All of the tested nanosystems exerted potent bactericidal activity in a manner resulting in the generation of ROS, followed by damage of the bacterial membranes and the leakage of intracellular content. Notably, the killing action occurred with all of the bacterial strains evaluated, including those known to be drug resistant, and at concentrations that did not impact the growth of host cells. Conclusions: Conjugation of CSA-131 with Au NPs by covalent bond between the COOH group from MHDA and NH3 from CSA-131 potentiates the antimicrobial activity of this ceragenin if compared to its action alone. Results validate the development of AuR NPs@CSA-131, AuP NPs@CSA-131, and AuS NPs@CSA-131 as potential novel nanoantibiotics that might effectively eradicate MDR bacteria.

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“…Her talk was inspiring and emphasized the vast avenue of new nano-pharmaceuticals being developed to treat other cancer types (Lopez et al, 2011 ). Next, León-Buitimea et al talked about the role of nanobioengineering in the development of smart therapeutics with programmed dynamic responses to target, release, and clear therapeutics in an efficient manner in the treatment of infectious diseases caused by virus and bacteria (León-Buitimea et al, 2020 ). Finally, Correa et al described the advances in her laboratory toward the design of hybrid composite nanoparticles that integrate enzymes to incorporate catalytic and superparamagnetic behaviors that would aid in designing drug delivery systems (Correa et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Reflections on the 2nd International Congress on NanoBioEngineering 2020

Morones‐Ramírez

2021

Front. Bioeng. Biotechnol.

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The Demand for New Antibiotics: Antimicrobial Peptides, Nanoparticles, and Combinatorial Therapies as Future Strategies in Antibacterial Agent Design

Cited by 197 publications

References 96 publications

Antimicrobial and Amyloidogenic Activity of Peptides. Can Antimicrobial Peptides Be Used against SARS-CoV-2?

Antimicrobial and Amyloidogenic Activity of Peptides. Can Antimicrobial Peptides Be Used against SARS-CoV-2?

Bactericidal Properties of Rod-, Peanut-, and Star-Shaped Gold Nanoparticles Coated with Ceragenin CSA-131 against Multidrug-Resistant Bacterial Strains

Reflections on the 2nd International Congress on NanoBioEngineering 2020

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