The delayed fluorescence kinetics as a method of biological tissue diagnostics

Scheglova

Anenkova

2015

JBPE

Abstract. The results of experimental investigations on the differences of wool microstructure and its biophysical properties of healthy mice and mice with malignant tumour are represented in the paper. It is shown that destruction of wool structure as well as biochemical composition change of wool cells is during pathology development. Difference is seen even when tumour is small. We have shown that the general regularity of spectral measurements for mammary and hair cells is conserved at health state and pathology present. Taking into account this, the represented data about wool structure can be used to improve the optical techniques for non--invasive diagnostics of cancer diseases at different stages of pathology. © 2015 Samara State Aerospace University (SSAU).

Section: The Results and Discussionsupporting

confidence: 81%

Change of Optical Properties of Hair Cells During Malignant Tumor Development

Scheglova

Anenkova

2015

JBPE

“…These results were obtained in according with our preview experimental results [7] for development of the optical techniques of biomedical diagnostics of pathologies.…”

Section: Introductionsupporting

confidence: 56%

“…Kinetics of the delayed fluorescence of the triplet probe located in normal and cancer mammalian cells are different [7] because of oxygen concentration changing within it. On the basis of the described processes, system of balance kinetic equations can be written as…”

Section: J Of Biomedical Photonics and Eng 2(3)mentioning

confidence: 99%

Long-term fluorescence of erythrosine in alive mammalian cells

2016

JBPE

Abstract. Investigation of triplet states of probes molecules located in cells and tissues is used for research of pathological processes included cancer development due to high sensitivity of triplet states lifetime to surround medium. In the work the long-term fluorescence of erythrosine located in alive mammalian cells has been analyzed by mathematical simulation. The model considers all basic processes of relaxation of probe triplet states in biological media. It is obtained that signal intensity and its form depends on oxygen concentration and viscosity of cells cytoplasm in which the dye diffuses. Interaction of erythrosine with the variety of extinguishers (included immobilization process into peptides molecules) does not impact on signal of the delayed fluorescence. Their concentration impact on cell viscosity mean only. The most important extinguisher is molecular oxygen impacting on signal. The correlation between theoretical and experimental results let to consider that the model is adequate.

“…Working in impulse periodic mode the solid-state YAG:Nd laser was used as the basis for the experimental setup for DF measurements [6]. For molecule excitation the second laser harmonic (λ ex = 532 nm) was used.…”

Section: Objects and Methods Of Researchmentioning

confidence: 99%

Pathology development stage and its influence on the delayed fluorescence kinetics of molecular probes

Letuta

2013

Laser Phys.

This paper describes regularity of the delayed fluorescence kinetics changes of xanthene dyes in the cancer cells received from tumors of different diameter. It shows that the rate constant of bimolecular reactions (singlet-triplet T− 1 g (O 2 ) and triplet-triplet annihilations) as well as thermoactive fluorescence decrease with increasing pathological process development stage. It could be a result of both more expressed cancer cell hypoxia and a change in cytoplasm viscosity.