The degradation and biocompatible study of polyester film in the gastrointestinal tract

Abstract

Application of biodegradable implants in the gastrointestinal tract is a new field of research; therefore, it is important to understand the degradation behavior of these biodegradable implants in vivo before being applied to clinical settings. In this paper, we provide a detailed protocol for thin film implantation into rat, so that their physiology can be studied. After polyester PCL film inoculation in the stomach and intestine, in vivo biofilm (PCL) degradation can be monitored. Therefore, the importance of this protocol is to study the host responses to biofilm degradation and to study the fundamental degradation properties of the biofilm. An emphasis is placed on surgical precision. The degradation of thin film in stomach and intestine are quantified using their morphology, which is evaluated using scanning electron microscopy. The implantation of biofilm in rat and their analyses can be completed using the proposed method in approximately 28 days. polyesters which are degraded by hydrolysis with or without enzyme. Surprisingly, more than thousands of papers being published in the biomaterials and tissue engineering literature which uses polyester in form of films and scaffolds, there is no in vivo degradation and biocompatible study of polyester in gastrointestinal (GI) tract. In this study, we have performed a method to study the degradable and toxicity of polyester in gastrointestinal (GI) tract.

Hydrogels Made of Poly-γ-Glutamic Acid and Sugar Alcohols for Enhanced Survival of Probiotic Strains Subjected to Low pH and Freeze Drying

“…However, under gastrointestinal tract conditions, there are considerably more that affect degradation rate. It is known that presence of enzymes accelerate degradation of ester bonds [25,26]. Therefore, it can be assumed that in the human gastrointestinal tract, degradation of hydrogels would perform faster.…”