The deformation of cancer cells through narrow micropores holds the potential to regulate genes that impact cancer malignancy

Abstract

Surgery, radiation, hormonal therapy, chemotherapy, and immunotherapy are standard treatment strategies for metastatic breast cancer. However, the heterogeneous nature of the disease poses challenges and continues to make it life-threatening....

Exploring novel immunotherapy biomarker candidates induced by cancer deformation

“…Our previous study designated MDA MB 231 Original cells as ’Ori’ and cells that underwent deformation after passing through a Transwell with a 3μm pore size as ’TW1.’ Subsequently, cells that underwent a second deformation by passing through a Transwell were labeled as ’TW2.’ Since TW1 and TW2 exhibited similar malignant characteristics, we conducted RNA sequencing exclusively on Ori and TW1 cells. Using a heatmap, we then examined the overall gene expression changes across all genes ( Fig 1B ) [ 43 ]. Using RNA sequencing data, we conducted a comparative gene expression analysis between Ori and TW1 cells.…”

“…In this current study, we utilized the Total RNA sequencing service provided by BGI ( https://www.bgi.com/us/home ), which had been previously employed in our previous research [ 43 ]. We identified differentially expressed genes among cells migrated through the microchannel using DESeq2 (ver 1.32.0).…”

“…In previous research [ 43 ], TW-1 and TW-2 cell lines were established from MDA-MB-23 cells and cultured using DMEM supplemented with 10% FBS (Gibco, 10270) and 1% penicillin-streptomycin (Gibco, 15140122) in a 5% CO2 incubator at 37°C.…”

“…Since TW1 and TW2 exhibited similar malignant characteristics, we conducted RNA sequencing exclusively on Ori and TW1 cells. Using a heatmap, we then examined the overall gene expression changes across all genes (Fig 1B) [43]. Using RNA sequencing data, we conducted a comparative gene expression analysis between Ori and TW1 cells.…”