The defined presentation of nanoparticles to cells and their surface controlled uptake

Alberola, Alicia Piera; Rädler, Joachim O.

doi:10.1016/j.biomaterials.2009.03.031

Cited by 31 publications

(27 citation statements)

References 24 publications

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“…In contrast to MSNs, the exocytosis of nonporous nanoparticles has been studied several times. Most of the reports on the exocytosis of nanoparticles have been based on two main approaches: microscopic tracking, and measurement of intra‐ and extracellular particle concentrations 36, 37, 39–43. These measurements have allowed the use of kinetic models to understand the dynamics of nanoparticle endo‐ and exocytosis 40.…”

Section: Methodsmentioning

confidence: 99%

Exocytosis of Mesoporous Silica Nanoparticles from Mammalian Cells: From Asymmetric Cell‐to‐Cell Transfer to Protein Harvesting

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Vivero‐Escoto

Zhao

et al. 2011

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The exocytosis of mesoporous silica nanoparticles (MSNs) from mammalian cells is demonstrated for the first time. The differences in the degree of exocytosis of MSNs between healthy and cancer cells are shown to be responsible for the asymmetric transfer of the particles between both cell types. The exocytosis of highly adsorbent magnetic MSNs proves to be useful as a means for harvesting biomolecules from living cells. Disciplines

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Section: Methodsmentioning

confidence: 99%

Exocytosis of Mesoporous Silica Nanoparticles from Mammalian Cells: From Asymmetric Cell‐to‐Cell Transfer to Protein Harvesting

Slowing

Vivero‐Escoto

Zhao

et al. 2011

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“…Folate-linking, however, increased the particle density from 1.3270 ± 0.0407 g cm −3 to 1.5960 ± 0.0725 g cm −3 (two-sided Student's t-test P < 0.05 for comparison, n = 3) which may influence particle uptake (Alberola and Radler, 2009). The HSA nanoparticles were of spherical shape (Fig.…”

Section: Characterisation Of Nanoparticlesmentioning

confidence: 97%

Interaction of folate-conjugated human serum albumin (HSA) nanoparticles with tumour cells

Ulbrich

Michaelis

Rothweiler

et al. 2011

International Journal of Pharmaceutics

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“…The results showed an increase in uptake upon aggregation (55). Surface presentation has been proposed as an original approach aiming at better controlled delivery conditions (56). Chithrani et al have reported that single 50 nm transferrin-coated gold nanoparticles are able to enter cells, while 14 nm transferrin-coated nanoparticles were only taken up when clustered in groups of at least six particles (57).…”

Section: Non-specific Cellular Uptakementioning

confidence: 99%

Gold nanoparticles delivery in mammalian live cells: a critical review

et al. 2010

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Functional nanomaterials have recently attracted strong interest from the biology community, not only as potential drug delivery vehicles or diagnostic tools, but also as optical nanomaterials. This is illustrated by the explosion of publications in the field with more than 2,000 publications in the last 2 years (4,000 papers since 2000; from ISI Web of Knowledge, ‘nanoparticle and cell’ hit). Such a publication boom in this novel interdisciplinary field has resulted in papers of unequal standard, partly because it is challenging to assemble the required expertise in chemistry, physics, and biology in a single team. As an extreme example, several papers published in physical chemistry journals claim intracellular delivery of nanoparticles, but show pictures of cells that are, to the expert biologist, evidently dead (and therefore permeable). To attain proper cellular applications using nanomaterials, it is critical not only to achieve efficient delivery in healthy cells, but also to control the intracellular availability and the fate of the nanomaterial. This is still an open challenge that will only be met by innovative delivery methods combined with rigorous and quantitative characterization of the uptake and the fate of the nanoparticles. This review mainly focuses on gold nanoparticles and discusses the various approaches to nanoparticle delivery, including surface chemical modifications and several methods used to facilitate cellular uptake and endosomal escape. We will also review the main detection methods and how their optimum use can inform about intracellular localization, efficiency of delivery, and integrity of the surface capping.

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The defined presentation of nanoparticles to cells and their surface controlled uptake

Cited by 31 publications

References 24 publications

Exocytosis of Mesoporous Silica Nanoparticles from Mammalian Cells: From Asymmetric Cell‐to‐Cell Transfer to Protein Harvesting

Exocytosis of Mesoporous Silica Nanoparticles from Mammalian Cells: From Asymmetric Cell‐to‐Cell Transfer to Protein Harvesting

Interaction of folate-conjugated human serum albumin (HSA) nanoparticles with tumour cells

Gold nanoparticles delivery in mammalian live cells: a critical review

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