The defective protein level of myosin light chain phosphatase (MLCP) in the isolated saphenous vein, as a vascular conduit in coronary artery bypass grafting (CABG), harvested from patients with diabetes mellitus (DM)

Diabetes Mellitus Induces Hyperreactivity of 5-Hydroxytryptamine (5-HT)-Induced Constriction in Human Internal Thoracic Artery and Is Associated with Increase in the Membrane Protein Level of 5-HT_2A Receptor

“…8) We previously reported that 5-HT-induced constriction was significantly augmented in human endothelium-denuded SV of patients with DM compared to those in patients without DM. 9,10) Furthermore, we reported that the level of the 5-HT 2A receptor in the membrane fraction of human SV smooth muscle cells did not change between patients with and without DM. 10) The internal thoracic artery (ITA) is the key graft conduit for CABG because of its excellent long-term patency.…”

Insulin Induces Internalization of the Plasma Membrane 5-Hydroxytryptamine2A (5-HT2A) Receptor in the Isolated Human Endothelium-Denuded Saphenous Vein via the Phosphatidylinositol 3-Kinase Pathway

“…We recently reported that in isolated human endothelium-denuded SVs, the constrictions caused by 5-HT were significantly greater (hyperreactivity) in the DM patients than in the non-DM patients (8). In the study, we demonstrated that the hyperreactivity to 5-HT in the SV smooth muscle of DM patients resulted from not only the enhanced phosphorylation of myosin light chain phosphatase (MLCP) but also the defective protein level of MLCP (8).…”

“…In the study, we demonstrated that the hyperreactivity to 5-HT in the SV smooth muscle of DM patients resulted from not only the enhanced phosphorylation of myosin light chain phosphatase (MLCP) but also the defective protein level of MLCP (8). Thus, we discovered that the defective protein levels of MLCP in the DM group may partially explain the poor patency of SV grafts harvested from DM patients.…”

Efficacy of Off-Pump Coronary Artery Bypass Grafting Using Skeletonized Bilateral Internal Thoracic Arteries in Diabetic Patients

“…In on-pump surgery, antiplatelet drugs are typically discontinued several days before surgery, and aspirin may not always be started within 6 hours of surgery because of bleeding problems. In addition, a defective protein level for myosin light chain phosphatase was observed in diabetic patients, which is regarded as one of the rationales for the poorer patency rate of saphenous vein grafts in diabetic patients 15) . This may partially explain the stronger favorable effects of bilateral ITA use in diabetic patients.…”