The decomposition of NAD+ under alkaline conditions

Hilvers, A.G.; Dam, K. Van

doi:10.1016/0926-6569(64)90056-2

Cited by 3 publications

(2 citation statements)

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“…However, we observed that the paper wells did not turn purple over time, indicating that INT and PMS were stable, and thus the NAD + likely degraded after immobilization on paper. The cleavage of the nicotinamide ring from NAD + at room temperature is well-known, though it is more pronounced in alkaline medium such as that used in our assay (pH 8.9). − …”

Section: Resultsmentioning

confidence: 90%

Printed Paper Sensors for Serum Lactate Dehydrogenase using Pullulan-Based Inks to Immobilize Reagents

et al. 2015

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In this study, a paper-based point-of-care (POC) colorimetric biosensor was developed for the detection of lactate dehydrogenase in serum using a nonporous, oxygen impermeable reversibly gelling polysaccharide material based on pullulan. The pullulan could be printed onto paper surfaces along with all required assay reagents, providing a means for high-stability immobilization of all reagents on paper. Serum containing lactate dehydrogenase (LDH) was directly spotted on to the pullulan-coated bioactive paper and provided quantitative colorimetric data that was comparable to that obtained with a conventional plate-reader method. The paper strip was found to be highly stable and could be stored at 4 °C for at least 10 weeks with no loss in performance, as compared to a complete loss in performance within 1 day when the reagents were printed without the stabilizing polysaccharide. The ease of fabrication coupled with the high stability of the printed reagents provides a facile platform for easily manufactured POC sensors.

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Section: Resultsmentioning

confidence: 90%

Printed Paper Sensors for Serum Lactate Dehydrogenase using Pullulan-Based Inks to Immobilize Reagents

et al. 2015

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“…The increase in the rate with further increase in pH from 8.5 results from thedeprotonation of the aqua complex (Ir-OH 2 , p K a = 9.5) to afford the hydroxo complex (Ir-OH) 3 , which may be active forthe oxidation of ethanol (Figure b). The pH range for thecatalytic hydrogenation of NAD + with ethanol in Figure b is limited because of the acid-catalyzed hydration of NADH under acidic conditions and decomposition of NAD + under alkaline conditions . The standard Gibbs energy change (Δ G ′°)for the reaction (eq ) at pH 7 at 298 K was determined to be +25 kJ mol –1 from the reported equilibrium constant .…”

Section: Resultsmentioning

confidence: 99%

Hydrogen Evolution from Aliphatic Alcohols and 1,4-Selective Hydrogenation of NAD⁺ Catalyzed by a [C,N] and a [C,C] Cyclometalated Organoiridium Complex at Room Temperature in Water

2012

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A [C,N] cyclometalated Ir complex, [Ir(III)(Cp*)(4-(1H-pyrazol-1-yl-κN(2))benzoic acid-κC(3))(H(2)O)](2)SO(4) [1](2)·SO(4), was reduced by aliphatic alcohols to produce the corresponding hydride complex [Ir(III)(Cp*)(4-(1H-pyrazol-1-yl-κN(2))-benzoate-κC(3))H](-)4 at room temperature in a basic aqueous solution (pH 13.6). Formation of the hydride complex 4 was confirmed by (1)H and (13)C NMR, ESI MS, and UV-vis spectra. The [C,N] cyclometalated Ir-hydride complex 4 reacts with proton to generate a stoichiometric amount of hydrogen when the pH was decreased to pH 0.8 by the addition of diluted sulfuric acid. Photoirradiation (λ > 330 nm) of an aqueous solution of the [C,N] cyclometalated Ir-hydride complex 4 resulted in the quantitative conversion to a unique [C,C] cyclometalated Ir-hydride complex 5 with no byproduct. The complex 5 catalyzed hydrogen evolution from ethanol in a basic aqueous solution (pH 11.9) under ambient conditions. The 1,4-selective catalytic hydrogenation of β-nicotinamide adenine dinucleotide (NAD(+)) by ethanol was also made possible by the complex 1 to produce 1,4-dihydro-β-nicotinamide adenine dinucleotide (1,4-NADH) at room temperature. The overall catalytic mechanism of hydrogenation of NAD(+), accompanied by the oxidation of ethanol, was revealed on the basis of the kinetic analysis and detection of the reaction intermediates.

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Effect of increments in the concentration of dopamine in the central nervous system on audiogenic seizures in DBA/2J mice

Dailey

Jobe

1984

Neuropharmacology

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The decomposition of NAD+ under alkaline conditions

Cited by 3 publications

References 9 publications

Printed Paper Sensors for Serum Lactate Dehydrogenase using Pullulan-Based Inks to Immobilize Reagents

Printed Paper Sensors for Serum Lactate Dehydrogenase using Pullulan-Based Inks to Immobilize Reagents

Hydrogen Evolution from Aliphatic Alcohols and 1,4-Selective Hydrogenation of NAD⁺ Catalyzed by a [C,N] and a [C,C] Cyclometalated Organoiridium Complex at Room Temperature in Water

Effect of increments in the concentration of dopamine in the central nervous system on audiogenic seizures in DBA/2J mice

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The decomposition of NAD+ under alkaline conditions

Cited by 3 publications

References 9 publications

Printed Paper Sensors for Serum Lactate Dehydrogenase using Pullulan-Based Inks to Immobilize Reagents

Printed Paper Sensors for Serum Lactate Dehydrogenase using Pullulan-Based Inks to Immobilize Reagents

Hydrogen Evolution from Aliphatic Alcohols and 1,4-Selective Hydrogenation of NAD+ Catalyzed by a [C,N] and a [C,C] Cyclometalated Organoiridium Complex at Room Temperature in Water

Effect of increments in the concentration of dopamine in the central nervous system on audiogenic seizures in DBA/2J mice

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Hydrogen Evolution from Aliphatic Alcohols and 1,4-Selective Hydrogenation of NAD⁺ Catalyzed by a [C,N] and a [C,C] Cyclometalated Organoiridium Complex at Room Temperature in Water