The decomposition of monofluorophosphate by enzymes in whole human saliva

Pearce, E.I.F.; Jenkins, G.N.

doi:10.1016/0003-9969(77)90064-4

Cited by 36 publications

(30 citation statements)

References 10 publications

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“…(2) Hydrolysis is inhibited by the low temperatures used during the centrifugation to obtain the fluid phase of plaque and saliva. (3) In agreement with other studies [Pearce and Jenkins, 1977], preliminary experiments also demonstrated that the rate of hydrolysis of MFP is slow in the centrifuged saliva and plaque fluid obtained after centrifugation. However, in spite of these observations, a small amount of MFP hydrolysis doubtlessly occurred prior to the determination of MFP content.…”

Section: Collection and Analysis Of Samplessupporting

confidence: 77%

“…The rate of release of fluoride by hydrolysis of MFP is pH-dependent and has been shown to occur most rapidly between 7.2 and 8.6 in saliva [Pearce and Jenkins, 1977]. However, it is unlikely that a specific pH effect would be a factor in the values presented here (other than the effect of the natural pH of the plaque and saliva samples), given the similarity of the pHs of these rinses and their low buffer capacities.…”

Section: Discussionmentioning

confidence: 43%

“…However, this acid is strong enough to recover nearly all the fluoride (samples re-extracted for 4 h with 1 mol/l HClO 4 recovered only about an additional mass fraction of 15% fluoride). The pH produced by this acidification is also low enough to Oral Fluoride after NaMFP or NaF Rinse Caries Res 2000;34: [404][405][406][407][408][409][410][411] inhibit the hydrolytic activity of phosphatase [Pearce and Jenkins, 1977].…”

Section: Collection and Analysis Of Samplesmentioning

confidence: 99%

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Fluoride in Plaque Fluid, Plaque, and Saliva Measured for 2 hours after a Sodium Fluoride Monofluorophosphate Rinse

Vogel

Y²,

Chow³

et al. 2000

Caries Res

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Sodium monofluorophosphate (NaMFP) and sodium fluoride (NaF) are the two most common sources of fluoride used in currently marketed fluoride dentifrices. The purpose of this study was to investigate the effect of mouth rinses containing NaF or NaMFP on the concentrations of fluoride, or the MFP ion, in saliva, whole plaque, and plaque fluid. Twelve subjects abstained from tooth brushing for 48 h, fasted overnight, and then rinsed 1 min with 12 mmol/l (228 ppm [μg/g] F) NaF or NaMFP in the morning. Before the rinse and at 30, 60 and 120 min afterwards, upper and lower molar and premolar plaque samples and whole saliva samples were collected. Aliquots of plaque fluid and centrifuged saliva were obtained from these samples, and the whole plaque residue acid extracted. The F and MFP concentrations were then measured in these samples using ultramicro methods. For both rinses, a higher concentration of plaque fluid fluoride was found at lower molar sites while the reverse was true for the whole plaque fluoride. Furthermore, for both rinses, plaque fluid, whole plaque, but not salivary, fluoride concentrations were above baseline at 120 min. Following the NaMFP rinse, a substantial amount of unhydrolyzed MFP was found in plaque fluid and saliva. Although there was a very large range in these measurements, fluoride in plaque fluid (excluding fluoride in unhydrolyzed MFP) and whole plaque were significantly (p<0.05) greater after the NaF rinse at all time periods. In saliva, the NaF rinse produced a statistically significant greater salivary fluoride (excluding fluoride in unhydrolyzed MFP) only at 60 min. The lack of a clear correlation between these measurements and clinical studies suggest a novel mechanism may enhance the effectiveness of NaMFP dentifrices.

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Section: Collection and Analysis Of Samplessupporting

confidence: 77%

Section: Discussionmentioning

confidence: 43%

Section: Collection and Analysis Of Samplesmentioning

confidence: 99%

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Fluoride in Plaque Fluid, Plaque, and Saliva Measured for 2 hours after a Sodium Fluoride Monofluorophosphate Rinse

Vogel

Y²,

Chow³

et al. 2000

Caries Res

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“…It has previously been shown that flora in saliva decompose MFP optimally at pH 7.2-8.6 [Pearce and Jenkins, 1977].…”

Section: Discussionmentioning

confidence: 99%

“…While the oral bacterial phosphatase effect on MFP is clear [Pearce and Jenkins, 1977], we are unaware of any measures that have been taken to ensure that toothpaste formulation and use are optimal for MFP hydrolysis. Fundamental properties of enzymes include effects of pH, temperature, substrate and enzyme (bacterial) concentration, all of which might be expected to influence MFP hydrolysis in the mouth.…”

mentioning

confidence: 99%

The Effect of pH, Temperature and Plaque Thickness on the Hydrolysis of Monofluorophosphate in Experimental Dental Plaque

Pearce¹,

Dibdin

2003

Caries Res

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Monofluorophosphate (MFP), an anti-caries agent commonly used in toothpaste, is known to be degraded to fluoride and orthophosphate by bacterial phosphatases in dental plaque. We have examined the effect of pH, temperature, plaque thickness and some ions on this process. Both natural plaque and artificial microcosm plaque incubated with purified MFP at pH 4–10 showed an optimum pH of ∼8 for hydrolysis. Diffusion and concomitant hydrolysis were examined in an apparatus in which artificial plaque was held between rigid membranes separating two chambers. When MFP diffused through a plaque of 0.51-mm thickness over 4 h it was almost completely hydrolysed at pH 8, but hydrolysis on diffusion decreased as the pH deviated from 8. MFP in toothpaste extract showed a similar pH susceptibility to hydrolysis, according to the inherent pH of the toothpaste. Hydrolysis of MFP in the toothpaste was reduced by no more than 10% when compared with a matched-pH control, suggesting that other toothpaste ingredients had no major influence on hydrolysis. Transport was slower and hydrolysis at pH 6 more complete the thicker the plaque, but hydrolysis was not significantly slower at 23°C than at 37°C. The addition of various potential activating or inhibiting ions at 0.1 and 1.0 mmol/l had small and non-significant effects on hydrolysis. The results suggest that MFP toothpaste should be formulated and used to maximise enzymic hydrolysis of this complex anion, and that plaque pH control is probably the most important factor.

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Is the dentifrice containing calcium silicate, sodium phosphate, and fluoride able to protect enamel against chemical mechanical wear? An in situ/ex vivo study

et al. 2019

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The decomposition of monofluorophosphate by enzymes in whole human saliva

Cited by 36 publications

References 10 publications

Fluoride in Plaque Fluid, Plaque, and Saliva Measured for 2 hours after a Sodium Fluoride Monofluorophosphate Rinse

Fluoride in Plaque Fluid, Plaque, and Saliva Measured for 2 hours after a Sodium Fluoride Monofluorophosphate Rinse

The Effect of pH, Temperature and Plaque Thickness on the Hydrolysis of Monofluorophosphate in Experimental Dental Plaque

Is the dentifrice containing calcium silicate, sodium phosphate, and fluoride able to protect enamel against chemical mechanical wear? An in situ/ex vivo study

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