The dawn of pirna research in various neuronal disorders

Wakisaka, Keiko Tsuji; Imai, Yuzuru

doi:10.2741/4789

Cited by 25 publications

(24 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results provide evidence of an essential role for the piRNA pathway in mammalian neurogenesis. This finding adds a new layer of complexity to the understanding of adult brain plasticity and aging, and entails crucial implications for neuronal disorders where dysregulated expression of the piRNA pathway has been reported, such as neurodegeneration (Jain et al, 2019;Wakisaka, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…In the adult hippocampus, a regulated balance of neural progenitor cells' (aNPCs) quiescence, proliferation and differentiation guarantees lifelong neurogenesis (1, 2), prevents the generation of reactive glia (3,4), and neurodegeneration (5). PIWI-interacting RNAs (piRNAs) are single-stranded noncoding RNAs of [21][22][23][24][25][26][27][28][29][30][31][32][33][34][35] nucleotides that, in gonads, target transposable elements (TEs) for degradation, thus maintaining germline stem cell pools and fertility (6,7). In the adult brain, the piRNA pathway was proposed to control synaptic plasticity and memory (8)(9)(10)(11).…”

mentioning

confidence: 99%

See 1 more Smart Citation

The piRNA pathway sustains adult neurogenesis by reducing protein synthesis and cellular senescence

Gasperini

Tuntevski

Pelizzoli

et al. 2020

Preprint

View full text Add to dashboard Cite

In specific niches of the adult mammalian brain, neural progenitor cells (aNPCs) ensure lifelong neurogenesis. Proper regulation of this process entails important implications for brain plasticity and health. We report that Piwil2 (Mili) and PIWI-interacting RNAs (piRNAs) are abundantly expressed in aNPCs but depleted in their progeny in the adult mouse hippocampus. Loss of function of the piRNA pathway in aNPCs inhibited neurogenesis and increased reactive gliogenesis in vivo and in vitro. PiRNA pathway depletion in cultured aNPCs increased levels of 5S ribosomal RNA, transfer RNAs and mRNAs encoding regulators of translation, resulting in higher polyribosome density and protein synthesis upon differentiation. We propose that the piRNA pathway sustains adult neurogenesis by repressing translation in aNPCs.One sentence summaryThe piRNA pathway is enriched in neural precursors and essential for appropriate neurogenesis by modulating translation

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

The piRNA pathway sustains adult neurogenesis by reducing protein synthesis and cellular senescence

Gasperini

Tuntevski

Pelizzoli

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Furthermore, pathogenic tau induced the reduction of piRNAs in tau-related neurodegenerative diseases such as AD and progressive supranuclear palsy and consequently caused abnormal transposable element activity and subsequently progressive neuronal death ( Sun et al, 2018 ). PiRNAs are thus beginning to be appreciated as pathogenic factors and therapeutic targets for neurodegenerative diseases as well as miRNAs ( Wakisaka and Imai, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Dysregulation of MicroRNAs and PIWI-Interacting RNAs in a Caenorhabditis elegans Parkinson’s Disease Model Overexpressing Human α-Synuclein and Influence of tdp-1

Shen¹,

Wang

Chen

et al. 2021

Front. Neurosci.

View full text Add to dashboard Cite

MicroRNAs (miRNAs) and PIWI-interacting RNAs (piRNAs) regulate gene expression and biological processes through specific genetic and epigenetic mechanisms. Recent studies have described a dysregulation of small non-coding RNAs in Parkinson’s disease (PD) tissues but have been limited in scope. Here, we extend these studies by comparing the dysregulation of both miRNAs and piRNAs from transgenic Caenorhabditis elegans (C. elegans) nematodes overexpressing pan-neuronally human α-synuclein wild-type (WT) (HASNWT OX) or mutant (HASNA53T OX). We observed 32 miRNAs and 112 piRNAs dysregulated in HASNA53T OX compared with WT. Genetic crosses of HASNA53T OX PD animal models with tdp-1 null mutants, the C. elegans ortholog of TDP-43, an RNA-binding protein aggregated in frontal temporal lobar degeneration, improved their behavioral deficits and changed the number of dysregulated miRNAs to 11 and piRNAs to none. Neuronal function-related genes T28F4.5, C34F6.1, C05C10.3, camt-1, and F54D10.3 were predicted to be targeted by cel-miR-1018, cel-miR-355-5p (C34F6.1 and C05C10.3), cel-miR-800-3p, and 21ur-1581 accordingly. This study provides a molecular landscape of small non-coding RNA dysregulation in an animal model that provides insight into the epigenetic changes, molecular processes, and interactions that occur during PD-associated neurodegenerative disorders.

show abstract

“…We have recently reported a correlation of snoRNAs with APOE genotype in postmortem brain tissue of AD patients [ (Lefterov et al, 2019); see also Figure 4]. Association of piRNAs with AD and neuronal activity (Wakisaka and Imai, 2019) or a suggestion for their use as CSF AD biomarkers in combination with miRNAs has been published (Jain et al, 2019;Wakisaka and Imai, 2019). The goal of our study was to determine if EV isolated from plasma of AD patients and nondemented individuals contain ncRNAs differentially associated with the disease.…”

Section: Discussionmentioning

confidence: 99%

Small nucleolar RNAs in plasma and their discriminatory power as diagnostic biomarkers of Alzheimer’s disease

Fitz

Wang

et al. 2021

Preprint

View full text Add to dashboard Cite

The clinical diagnosis of Alzheimer's disease, at its early stage, remains a difficult task. Advanced imaging technologies and laboratory assays to detect Aβ; peptides Aβ42 and Aβ40, total and phosphorylated tau in CSF provide a set of biomarkers of developing AD brain pathology and facilitate the diagnostic process. The search for biofluid biomarkers, other than in CSF, and the development of biomarker assays have accelerated significantly and now represent the fastest-growing field in AD research. The goal of this study was to determine the differential enrichment of noncoding RNAs (ncRNAs) in plasma-derived extracellular vesicles (EV) of AD patients and Cognitively Normal controls (NC). Using RNA-seq, we profiled four significant classes of ncRNAs: miRNAs, snoRNAs, tRNAs, and piRNAs. We report a significant enrichment of SNORDs - a group of snoRNAs, in AD samples compared to NC. To verify the differential enrichment of two clusters of SNORDs - SNORD115 and SNORD116, localized on human chromosome 15q11-q13, we used plasma samples of an independent group of AD patients and NC. We applied ddPCR technique and identified SNORD115 and SNORD116 with a high discriminatory power to differentiate AD samples from NC. The results of our study present evidence that AD is associated with changes in the enrichment of SNORDs, transcribed from imprinted genomic loci, in plasma EV and provide a rationale to further explore the validity of those SNORDs as plasma biomarkers of AD.

show abstract

The dawn of pirna research in various neuronal disorders

Cited by 25 publications

References 0 publications

The piRNA pathway sustains adult neurogenesis by reducing protein synthesis and cellular senescence

The piRNA pathway sustains adult neurogenesis by reducing protein synthesis and cellular senescence

Dysregulation of MicroRNAs and PIWI-Interacting RNAs in a Caenorhabditis elegans Parkinson’s Disease Model Overexpressing Human α-Synuclein and Influence of tdp-1

Small nucleolar RNAs in plasma and their discriminatory power as diagnostic biomarkers of Alzheimer’s disease

Contact Info

Product

Resources

About