The DamX protein of<i>Escherichia coli</i>and<i>Salmonella enterica</i>

López‐Garrido, Javier; Casadesús, Josep

doi:10.4161/gmic.1.4.12079

Cited by 11 publications

(7 citation statements)

References 31 publications

(42 reference statements)

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“…DamX has recently been shown to interact with the essential division protein FtsQ and is thought to contribute to cytokinesis [55]. Null mutations of dam X render E. coli sensitive to bile salts, suggesting that dam X is important for survival in the gut environment [56]. Other genes identified in this screen which have previously been identified to have a role in cell division include genes from the histidine biosynthesis operon [57], and the Rac prophage-enocoded Kil protein [58].…”

Section: Discussionmentioning

confidence: 99%

Harnessing Single Cell Sorting to Identify Cell Division Genes and Regulators in Bacteria

et al. 2013

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Cell division is an essential cellular process that requires an array of known and unknown proteins for its spatial and temporal regulation. Here we develop a novel, high-throughput screening method for the identification of bacterial cell division genes and regulators. The method combines the over-expression of a shotgun genomic expression library to perturb the cell division process with high-throughput flow cytometry sorting to screen many thousands of clones. Using this approach, we recovered clones with a filamentous morphology for the model bacterium, Escherichia coli. Genetic analysis revealed that our screen identified both known cell division genes, and genes that have not previously been identified to be involved in cell division. This novel screening strategy is applicable to a wide range of organisms, including pathogenic bacteria, where cell division genes and regulators are attractive drug targets for antibiotic development.

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Section: Discussionmentioning

confidence: 99%

Harnessing Single Cell Sorting to Identify Cell Division Genes and Regulators in Bacteria

et al. 2013

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“…damX , dam , rpe , aroB , uvrD , and yigB were required for fitness under pH 3, starvation, and H 2 O 2 . Disruption of damX in S. enterica causes bile sensitivity ( 79 ). The DNA adenine methylation gene ( dam ) plays an important role in bacterial gene expression and virulence ( 80 ).…”

Section: Resultsmentioning

confidence: 99%

Genetic Determinants in Salmonella enterica Serotype Typhimurium Required for Overcoming In Vitro Stressors in the Mimicking Host Environment

2021

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“…damX is part of a functionally diverse operon comprised of aroBK , damX , dam , rpe , gph , and trpS . Some previous studies suggested that polarity effects of damX deletion on the downstream dam gene, which encodes the Dam DNA methylase, could account for the phenotypes associated with damX deletion, due to a likely σ 70 damp1 promoter within the damX open reading frame ( 39 – 41 ). Analysis of dam , aroBK , and damX expression levels in the UTI89Δ damX mutant relative to the UTI89 wild type revealed no significant effect of damX deletion on dam expression (see Fig.…”

Section: Resultsmentioning

confidence: 99%

DamX Controls Reversible Cell Morphology Switching in Uropathogenic Escherichia coli

Khandige

Asferg

Rasmussen

et al. 2016

mBio

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The ability to change cell morphology is an advantageous characteristic adopted by multiple pathogenic bacteria in order to evade host immune detection and assault during infection. Uropathogenic Escherichia coli (UPEC) exhibits such cellular dynamics and has been shown to transition through a series of distinct morphological phenotypes during a urinary tract infection. Here, we report the first systematic spatio-temporal gene expression analysis of the UPEC transition through these phenotypes by using a flow chamber-based in vitro infection model that simulates conditions in the bladder. This analysis revealed a novel association between the cell division gene damX and reversible UPEC filamentation. We demonstrate a lack of reversible bacterial filamentation in a damX deletion mutant in vitro and absence of a filamentous response by this mutant in a murine model of cystitis. While deletion of damX abrogated UPEC filamentation and secondary surface colonization in tissue culture and in mouse infections, transient overexpression of damX resulted in reversible UPEC filamentation. In this study, we identify a hitherto-unknown damX-mediated mechanism underlying UPEC morphotypical switching. Murine infection studies showed that DamX is essential for establishment of a robust urinary tract infection, thus emphasizing its role as a mediator of virulence. Our study demonstrates the value of an in vitro methodology, in which uroepithelium infection is closely simulated, when undertaking targeted investigations that are challenging to perform in animal infection models.

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The DamX protein ofEscherichia coliandSalmonella enterica

Cited by 11 publications

References 31 publications

Harnessing Single Cell Sorting to Identify Cell Division Genes and Regulators in Bacteria

Harnessing Single Cell Sorting to Identify Cell Division Genes and Regulators in Bacteria

Genetic Determinants in Salmonella enterica Serotype Typhimurium Required for Overcoming In Vitro Stressors in the Mimicking Host Environment

DamX Controls Reversible Cell Morphology Switching in Uropathogenic Escherichia coli

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