2007
DOI: 10.1242/jcs.013698
|View full text |Cite
|
Sign up to set email alerts
|

The Dam1/DASH complex is required for the retrieval of unclustered kinetochores in fission yeast

Abstract: In fission yeast centromeres cluster at the nuclear envelope in a region underlying the spindle pole body during interphase, an arrangement known as a Rabl configuration. We have identified a strain in which one pair of sister kinetochores is unclustered from the others and binds the nuclear envelope at a point distal to the spindle pole body. We show that during mitosis unclustered kinetochores are captured by intranuclear spindle microtubules which then pull the kinetochores back to one of the two spindle po… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1

Citation Types

4
59
1

Year Published

2008
2008
2015
2015

Publication Types

Select...
8

Relationship

2
6

Authors

Journals

citations
Cited by 50 publications
(64 citation statements)
references
References 45 publications
4
59
1
Order By: Relevance
“…1, step 2, end-on pulling; Fig. 2b; Franco et al 2007;Tanaka et al 2007;Gachet et al 2008). Biochemical reconstitution revealed that about 16 Dam1 complexes oligomerize and form a ring that encircles a microtubule ( Fig.…”
Section: Interface Of Kinetochore Microtubule Interaction: the Ndc80 mentioning
confidence: 99%
See 1 more Smart Citation
“…1, step 2, end-on pulling; Fig. 2b; Franco et al 2007;Tanaka et al 2007;Gachet et al 2008). Biochemical reconstitution revealed that about 16 Dam1 complexes oligomerize and form a ring that encircles a microtubule ( Fig.…”
Section: Interface Of Kinetochore Microtubule Interaction: the Ndc80 mentioning
confidence: 99%
“…The lateral microtubule surface, known as the lattice, provides much larger contact surface, compared with microtubule tips, thus, contributing to an efficient first encounter with kinetochores. The capture of microtubule lattice by kinetochores was initially discovered in newt lung cells (Hayden et al 1990;Rieder and Alexander 1990) and subsequently found in budding yeast and fission yeast (Tanaka et al 2005a;Franco et al 2007;Gachet et al 2008); therefore, this mode of the capture is widely conserved among eukaryotic cells.…”
Section: Introductionmentioning
confidence: 99%
“…Kinetochores include MT-dependent motors and several protein complexes that lack motor activity but can still bind to a MT wall or end. These nonmotor complexes too may be important for chromosome motion, because depolymerizing MTs can generate enough force to move chromosomes without the help of motors, both in vivo and in vitro (4)(5)(6)(7).…”
mentioning
confidence: 99%
“…20). Similar to ScDam1, SpDam1 is localized at kinetochores as well as on the spindle and the intranuclear MT (20)(21)(22), and functions in coupling kinetochores to MTs (22)(23)(24). Using quantitative fluorescence microscopy, it was determined that on average only seven to eight copies of SpDam1 per kinetochore were found (25).…”
mentioning
confidence: 99%