The D genome carries a gene determining purple grain colour in wheat

Tereshchenko, O. Y.; Gordeeva, E. I.; Арбузова, В. С.; Börner, Andreas; Хлесткина, Е. К.

doi:10.1556/crc.40.2012.3.2

Cited by 47 publications

(44 citation statements)

References 13 publications

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“…1). This location fits well those of Pp-D1 on chromosome 7D (Tereshchenko et al 2012a) and Pp-B1 on chromosome 7B (Khlestkina et al 2010a).…”

Section: Discussionsupporting

confidence: 82%

“…tetraploid (durum) wheat chromosome arm 2AL also harbours a probable Pp3 orthologue (Khlestkina et al 2010a). Durum wheat also houses Pp-B1 (complementary to Pp3), mapping to chromosome arm 7BS (Khlestkina et al 2010a), while its D-genomic homoeologue in bread wheat (Pp-D1) lies on chromosome arm 7DS (Tereshchenko et al 2012a). Based on an analysis of introgression lines, Tereshchenko et al (2012b) have shown that a functional allele of at least one purple pericarp gene is retained in both Aegilops speltoides and Triticum timopheevii, although in the latter species, the purple grain trait has to date never been described (Tereshchenko et al 2012b).…”

Section: Introductionmentioning

confidence: 99%

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Marker-assisted development of bread wheat near-isogenic lines carrying various combinations of purple pericarp (Pp) alleles

2014

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The commercial interest in pigmented wheat grain flows from an understanding that they are nutritionally superior to white kernels. The pigment of purple coloured bread and durum wheat grains results from the accumulation of anthocyanins in the pericarp; its genetic basis is the action of Pp-1 and Pp3 genes. Here, the development of a set of bread wheat near isogenic lines (NILs) carrying various combinations of Pp alleles is described, along with a demonstration of their utility for the genetic dissection of the purple pericarp trait. A marker-assisted backcrossing strategy was based on the use of microsatellite markers linked to Pp3 (chromosome 2A), Pp-A1 (7A) and Pp-D1 (7D). Pp-A1 is a newly uncovered gene of weak effect. A qRT-PCR-based analysis of the anthocyanin synthesis structural genes [Chi (chalconeflavanone isomerase) and F3h (flavanone 3-hydroxylase)] transcript abundance in the pericarp of the NILs suggested that the Pp genes up-regulate their transcription in contrasting ways. These NILs represent a resource for studying the effect of grain pigmentation on other wheat traits and end products.

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“…1). This location fits well those of Pp-D1 on chromosome 7D (Tereshchenko et al 2012a) and Pp-B1 on chromosome 7B (Khlestkina et al 2010a).…”

Section: Discussionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Marker-assisted development of bread wheat near-isogenic lines carrying various combinations of purple pericarp (Pp) alleles

2014

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“…The above-mentioned stresses occur rarely in the cultivated field of Qinghai province, and red coleoptile should have no more advantage over uncoloured coleoptile in this condition. Moreover, red coleoptile (Rc-1) and the genes for purple grain (Pp-1) are close linked on the chromosomes of homoeologous group 7 Tereshchenko et al 2012), and purple grain can influence flour colour, which reduce the visual and taste sensation of flour (Wende et al 2007). Selection against purple grain leads to co-selection against red coleoptile.…”

Section: Discussionmentioning

confidence: 99%

Frame-shift mutation causes the function loss of TaMYB-A1 regulating anthocyanin biosynthesis in Triticum aestivum

Wei

Chen

et al. 2017

Cereal Research Communications

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Red coleoptile is an easily observed trait in Triticum aestivum and can provide some protection against stress. Here, TaMYB-A1 or TuMYB-A1, homologous to TaMYB-D1, which controls red coleoptile formation in the common wheat cultivar 'Gy115', was isolated from eight T. aestivum and 34 T. urartu cultivars. The genome sequence of TaMYB-A1 was 867 bp with an intron of 93 bp, which was similar to the MYBs regulating anthocyanin biosynthesis in T. aestivum but different from other MYB transcription factors regulating anthocyanin biosynthesis. TaMYB-A1 had an integrated DNA-binding domain of 102 amino acids and a transcriptional domain of 42 amino acids, which was responsible for regulating anthocyanin biosynthesis. TaMYB-A1 was assigned to the same branch as the MYBs regulating anthocyanin biosynthesis in a phylogenetic tree. A transient expression analysis showed that TaMYB-A1 induced 'Opata' coleoptile cells to synthesize anthocyanin with the help of ZmR. A non-functional allele of TaMYB-a1 existed in common wheat cultivars containing rc-a1. One single nucleotide was deleted 715 bp after the start codon in TaMYB-a1 compared with TaMYB-A1. The deletion caused a frame shift mutation, destroyed the DNA transcription activator domain, and resulted in TaMYB-a1 losing its ability to regulate anthocyanin biosynthesis in 'Opata' coleoptile cells. Those cultivars with functional TaMYB-A1 or TuMYB-A1 have red coleoptiles. The isolation of TaMYB-A1 should aid in understanding the molecular mechanisms of coleoptile traits in T. aestivum.

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“…These lines, designated i:S29Pp-A1Pp-D1Pp3 PF ('iPF') and i:S29Pp-A1Pp-D1Pp3 P ('iP'), respectively, have been characterized earlier using microsatellite markers, and differ only in the length of the introgressed fragments in the chromosomes 2A and 7D (Tereshchenko et al 2012;Gordeeva et al 2015).…”

Section: Plant Materialsmentioning

confidence: 99%

Anthocyanins participate in protection of wheat seedlings from osmotic stress

Shoeva

Gordeeva

Арбузова

et al. 2017

Cereal Research Communications

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Plant secondary metabolites anthocyanins are considered to play a protective role. In bread wheat (Triticum aestivum L.), anthocyanins can be observed in both adult plants and seedlings. The aim of the current study was to investigate the putative role of anthocyanins present in grains and shoots with respect to the protection of seedlings against drought. For this purpose a set of near isogenic lines (NILs) differing in pericarp and coleoptile colour was used. Water stress was created by artificial shortage of moisture under laboratory conditions. Differences among the lines were observed in a way that the lines with dark-purple grains and coleoptiles (genotype Pp-D1Pp-D1Pp3Pp3Rc-A1Rc-A1Rc-D1Rc-D1) demonstrated a higher seedling drought tolerance than plants with uncoloured pericarp and lightpurple coleoptiles (pp-D1pp-D1pp3pp3Rc-A1Rc-A1rc-D1rc-D1). Furthermore, protection of the root system and the shoot was related with the presence of anthocyanins in grains and coleoptiles, respectively.

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The D genome carries a gene determining purple grain colour in wheat

Cited by 47 publications

References 13 publications

Marker-assisted development of bread wheat near-isogenic lines carrying various combinations of purple pericarp (Pp) alleles

Marker-assisted development of bread wheat near-isogenic lines carrying various combinations of purple pericarp (Pp) alleles

Frame-shift mutation causes the function loss of TaMYB-A1 regulating anthocyanin biosynthesis in Triticum aestivum

Anthocyanins participate in protection of wheat seedlings from osmotic stress

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