The cytotoxicity to leukemia cells and antiviral effects of Isatis indigotica extracts on pseudorabies virus

Hsuan, Shih-Ling; Chang, Shih‐Chieh; Wang, Sheng-Yang; Liao, Tien-Ling; Jong, Ting‐Ting; Chien, Maw-Sheng; Lee, Wei‐Cheng; Chen, Shih-Shiung; Liao, Jiunn‐Wang

doi:10.1016/j.jep.2009.02.028

Cited by 73 publications

(40 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…50 Another derivative was shown to inhibit viral replication of H5N1 51 , cytomegalovirus 52 , and the pseudorabies virus. 53 These reports and the results within this study, suggest that the effects of indirubin on viral replication may depend on the indirubin-derivative used, and/or on type of virus investigated. In our study, indirubin consistently augmented adenoviral oncolysis in multiple GSC cultures and other cancer cell lines, as well as the oncolytic activity of two other viruses, NDV and HSV.…”

Section: Discussionmentioning

confidence: 58%

In vitro screening of clinical drugs identifies sensitizers of oncolytic viral therapy in glioblastoma stem-like cells

et al. 2015

View full text Add to dashboard Cite

Oncolytic viruses (OV) have broad potential as an adjuvant for the treatment of solid tumors. The present study addresses the feasibility of clinically applicable drugs to enhance the oncolytic potential of the OV Delta24-RGD in glioblastoma. In total, 446 drugs were screened for their viral sensitizing properties in glioblastoma stem-like cells (GSCs) in vitro. Validation was done for 10 drugs to determine synergy based on the Chou Talalay assay. Mechanistic studies were undertaken to assess viability, replication efficacy, viral infection enhancement and cell death pathway induction in a selected panel of drugs. Four viral sensitizers (fluphenazine, indirubin, lofepramine and ranolazine) were demonstrated to reproducibly synergize with Delta24-RGD in multiple assays. After validation, we underscored general applicability by testing candidate drugs in a broader context of a panel of different GSCs, various solid tumor models and multiple OVs. Overall, this study identified four viral sensitizers, which synergize with Delta24-RGD and two other strains of OVs. The viral sensitizers interact with infection, replication and cell death pathways to enhance efficacy of the OV.

show abstract

Section: Discussionmentioning

confidence: 58%

In vitro screening of clinical drugs identifies sensitizers of oncolytic viral therapy in glioblastoma stem-like cells

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Additionally, indigodole B and the S. cusia leaf methanol extract exhibited effective virucidal activity against HCoV-NL63, with IC50 values of 2.09 μM for indigodole B and 0.12 μg/mL for S. cusia extract ( Figure 10B,C, and Table 1). Since tryptanthrin was one of the critical components in Persicaria tinctoria and Isatis tinctoria (Isatis indigotica), it might play a role in the antiviral properties of the extracts against Influenza A and B, dengue, Japanese encephalitis, and pseudorabies virus [11,[40][41][42][43]. Since tryptanthrin was one of the critical components in Persicaria tinctoria and Isatis tinctoria (Isatis indigotica), it might play a role in the antiviral properties of the extracts against Influenza A and B, dengue, Japanese encephalitis, and pseudorabies virus [11,[40][41][42][43].…”

Section: Discussionmentioning

confidence: 99%

Antiviral Action of Tryptanthrin Isolated from Strobilanthes cusia Leaf against Human Coronavirus NL63

et al. 2020

View full text Add to dashboard Cite

Strobilanthes cusia (Nees) Kuntze is a Chinese herbal medicine used in the treatment of respiratory virus infections. The methanol extract of S. cusia leaf contains chemical components such as β-sitosterol, indirubin, tryptanthrin, betulin, indigodole A, and indigodole B that have diverse biological activities. However, the antiviral action of S. cusia leaf and its components against human coronavirus remains to be elucidated. Human coronavirus NL63 infection is frequent among immunocompromised individuals, young children, and in the elderly. This study investigated the anti-Human coronavirus NL63 (HCoV-NL63) activity of the methanol extract of S. cusia leaf and its major components. The methanol extract of S. cusia leaf effectively inhibited the cytopathic effect (CPE) and virus yield (IC50 = 0.64 μg/mL) in HCoV-NL63-infected cells. Moreover, this extract potently inhibited the HCoV-NL63 infection in a concentration-dependent manner. Among the six components identified in the methanol extract of S. cusia leaf, tryptanthrin and indigodole B (5aRethyltryptanthrin) exhibited potent antiviral activity in reducing the CPE and progeny virus production. The IC50 values against virus yield were 1.52 μM and 2.60 μM for tryptanthrin and indigodole B, respectively. Different modes of time-of-addition/removal assay indicated that tryptanthrin prevented the early and late stages of HCoV-NL63 replication, particularly by blocking viral RNA genome synthesis and papain-like protease 2 activity. Notably, tryptanthrin (IC50 = 0.06 μM) and indigodole B (IC50 = 2.09 μM) exhibited strong virucidal activity as well. This study identified tryptanthrin as the key active component of S. cusia leaf methanol extract that acted against HCoV-NL63 in a cell-type independent manner. The results specify that tryptanthrin possesses antiviral potential against HCoV-NL63 infection.

show abstract

“…"Ban lan gen" (Isatis indigotica root) is one of the most important traditional Chinese medicines used for the treatment of influenza and various infectious diseases [17] as well as anti-severe acute respiratory syndrome (SARS) [18]. Previous studies demonstrated that indirubin, one of the bioactive indole alkaloids of the herbal drug, had the most significant cytotoxicity on IL-60 cells and inhibitory effect on pesudorabies virus (PrV) replication, while extracts from roots and leaves of I. indigotica also showed cytopathic effect (CPE) reduction either before or after infection of PrV on porcine kidney (PK-15) cells [18]. Moreover, from the ethanol extract of "da qing ye" (I. indigotica leaves) an isatisine Aderived artificial acetonide was reported to have a moderate anti-HIV-1 activity (EC 50 ¼ 37.8 mM) [19].…”

Section: Introductionmentioning

confidence: 99%

Discovery, synthesis, and optimization of an N-alkoxy indolylacetamide against HIV-1 carrying NNRTI-resistant mutations from the Isatis indigotica root

Chen

Xin

Chen

et al. 2020

European Journal of Medicinal Chemistry

View full text Add to dashboard Cite

a b s t r a c tFrom an aqueous decoction of the traditional Chinese medicine "ban lan gen" (the Isatis indigotica root), an antiviral natural product CI -39 was isolated as an NNRTI (non-nucleoside reverse transcriptase inhibitor) (EC 50 ¼ 3.40 mM). Its novel structure was determined as methyl (1-methoxy-1H-indol-3-yl) acetamidobenzoate by spectroscopic data and confirmed by single crystal X-ray diffraction. Through synthesis and structure-activity relationship (SAR) investigation of CI -39 and 57 new derivatives (24 with EC 50 values of 0.06e8.55 mM), two optimized derivatives 10f and 10i (EC 50 : 0.06 mM and 0.06 mM) having activity comparable to that of NVP (EC 50 ¼ 0.03 mM) were obtained. Further evaluation verified that 10f and 10i were RT DNA polymerase inhibitors and exhibited better activities and drug resistance folds compared to NVP against seven NNRTI-resistant strains carrying different mutations. Especially, 10i(EC 50 ¼ 0.43 mM) was more active to the L100I/K103N double-mutant strain as compared to both NVP (EC 50 ¼ 0.76 mM) and EFV (EC 50 ¼ 1.08 mM). The molecular docking demonstrated a possible binding pattern between 10i and RT and revealed activity mechanism of 10i against the NNRTI-resistant strains. a Chemical shift values (d) were measured at 500 MHz for 1 H and at 125 MHz for 13 C, respectively. Proton coupling constants (J) in Hz are given in parentheses. The assignments were based on DEPT, 1 He 1 H COSY, HSQC, and HMBC experiments.C. Xu et al.

show abstract

The cytotoxicity to leukemia cells and antiviral effects of Isatis indigotica extracts on pseudorabies virus

Abstract: Isatis indigotica posses a valuable virucidal effect in disease control of pseudorabies virus infection in swine.

Cited by 73 publications

References 18 publications

In vitro screening of clinical drugs identifies sensitizers of oncolytic viral therapy in glioblastoma stem-like cells

In vitro screening of clinical drugs identifies sensitizers of oncolytic viral therapy in glioblastoma stem-like cells

Antiviral Action of Tryptanthrin Isolated from Strobilanthes cusia Leaf against Human Coronavirus NL63

Discovery, synthesis, and optimization of an N-alkoxy indolylacetamide against HIV-1 carrying NNRTI-resistant mutations from the Isatis indigotica root

Contact Info

Product

Resources

About