The Cytotoxicity of Some Organic Solvents on Isolated Hepatocytes in Monolayer Culture

Zapór, Lidia; Skowroń, Jolanta; Gołofit-Szymczak, Małgorzata

doi:10.1080/10803548.2002.11076520

Cited by 11 publications

(10 citation statements)

References 10 publications

(4 reference statements)

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“…MTT test, supported by a strong body of literature, is a metabolic activity assay which involves the use of tetrazolium salt cleaved in the mitochondria of metabolically active cells to colored formazan salt that can be measured by absorbance. The amount of formazan dye produced is directly proportional to the quantity of metabolically active cells [49–51]. …”

Section: Discussionmentioning

confidence: 99%

Combined treatment with quercetin and imperatorin as a potent strategy for killing HeLa and Hep-2 cells

et al. 2014

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The aim of the present study was to assess the effect of quercetin and imperatorin administered separately and in combination on apoptosis and autophagy induction in human cervical carcinoma HeLa cells and laryngeal carcinoma Hep-2 cells cultured in vitro. Conducted MTT measurements proved that quercetin and imperatorin displayed a strong antiproliferative activity manifested in markedly reduction of HeLa and Hep-2 cells viability as a result of treatment with 50 μM of each compound. Further cell staining assays revealed that concentration mentioned above generated the highest percentage of apoptotic cells especially in the case of application of both drugs for 48 h. Simultaneous quercetin and imperatorin administration induced apoptosis remarkably stronger than treatment with single drugs. Experiments at the molecular level confirmed these results accompanied with the decreased Hsp27 and Hsp72 expression and, in addition, with increased caspases activity. Autophagy was not observed and no significant changes in the expression of beclin-1 were noticed. Additionally, experiments were performed on the above-mentioned cell lines with blocked Hsp27 and Hsp72 expression. In these cells, no significant changes in the sensitivity to apoptosis induction upon quercetin and imperatorin treatment were observed. The present study has provided evidence supporting the potential of the combination of quercetin and imperatorin drugs as a novel tool to be used in anticancer therapy. Our results have also demonstrated that blocking of the Hsp27 and Hsp72 gene expression is not enough to sensitize cancer cells to programmed cell death induction in HeLa and Hep-2 cells.

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Section: Discussionmentioning

confidence: 99%

Combined treatment with quercetin and imperatorin as a potent strategy for killing HeLa and Hep-2 cells

et al. 2014

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“…Since many of these compounds are waterinsoluble, the use of organic solvent is necessary (4). However, a common concern when introducing organic solvents to the growth medium is toxic effects of the solvent on cells (2,5,8,9). In addition, using unsuitable solvents might cause the loss of biological activities in compounds; therefore, it may seriously affect the outcome of the experiment (1, 3).…”

Section: Discussionmentioning

confidence: 99%

“…Some of the commonly used organic solvents such as acetone (Log P, -0.24), ethanol (Log P, -0.31), dimethyl sulfoxide (DMSO) (Log P, -1.35) and dimethyl formamide (DMF) (Log P, -1.01) were selected in the current study. Based on reports from in vitro studies, possible toxic effects of these organic solvents are expected (5)(6)(7). Several authors have reported interference of frequently used solvents with cellular based assays (8,9).…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic Effects of Some Common Organic Solvents on MCF-7, RAW-264.7 and Human Umbilical Vein Endothelial Cells

Jamalzadeh¹,

Ghafoori²,

Sariri³

et al. 2016

Avicenna J Med Biochem

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Background: Organic solvents are widely used in cell biology experiments. Despite increasing the solubility, they have some moderate toxic effects. Therefore, selecting the appropriate solvent along with the use of suitable concentration insures the accuracy and reliability of experimental results. Objectives: The current study aimed to examine the cytotoxic effects of some organic solvents on various cell models including MCF-7, RAW-264.7 and human umbilical vein endothelial cells (HUVEC). Materials and Methods:To evaluate the cytotoxicity effect of common organic solvents on the MCF-7, RAW-264.7 and HUVEC cells, multi-table tournament (MTT) colorimetric assay, the widely used and validated cytotoxicity test was applied. For this purpose, the selected cells were treated with different concentrations (0, 0.1%, 0.5%, 1%, 1.5%, 2%, 3% and 5% v/v) of four most commonly used organic solvents (acetone, ethanol, dimethyl sulfoxide (DMSO) and dimethylformamide (DMF) and then subjected to MTT experiment. Results: According to the obtained results, the cytotoxicity increased significantly with increasing the concentration of all four solvents compared to that of the control group. Studies with MCF-7, RAW-264.7 and HUVEC suggested that acetone, ethanol and DMSO at concentrations of 0.1% and 0.5%, had little or no toxicity, whereas higher concentrations inhibited the growth of all three cells. Compared with other three solvents, DMF displayed rather greater toxicity. Based on the results, proliferation of MCF-7, RAW-264.7 and HUVEC cells were inhibited by all used organic solvents, dose dependently. Conclusions: Thus, the background experimental error can be reduced remarkably by maximal concentration of 0.5% ethanol, acetone and DMSO and 0.1% DMF in the final treatment medium.

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“…For the assessment of acute effects of thinner treatment on neuronal cells and the evaluation of the dose-and time-response dependencies, we added different concentration of thinner (0.0%, 0.2%, 0.4% and 0.8%) to the culture medium for different time of incubation (1h, 6h and 24h). In order to improve the solubility of thinner compounds and therefore facilitate their absorption by the cells, the tested mixture was dissolved directly in culture medium with fetal bovine serum (10%) [30]. In fact, to dissolve thinner, we have avoided the use in our experiments DMSO or alcohol in order to prevent the cytotoxic action of these compounds [31,32,33].…”

Section: Discussionmentioning

confidence: 99%

“…In fact, to dissolve thinner, we have avoided the use in our experiments DMSO or alcohol in order to prevent the cytotoxic action of these compounds [31,32,33]. It should be pointed out that during the current protocol we used a free mineral oil medium, since it has been proven that this oil could dramatically affect neurons and astrocytes viability because of their intense metabolism [30]. Interestingly, cytotoxic potential of the tested solvent cannot be disrupted following the addition of fetal bovine serum to cell culture medium.…”

Section: Discussionmentioning

confidence: 99%

Acute Paint Thinner Treatment Induces Cell Death in Cultured Neurons and Astrocytes

Malloul¹

2016

IJRET

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Background: Paint thinner is a highly toxic chemical solvent, that when inhaled has many psychoactive properties. Chronic abuse of inhalants in humans leads to pronounced neurobiological and neuropsychological impairments; however, limited studies have demonstrated the cytotoxic effects of exposure to organic solvents in its complex mixture form. Objectives: Neuronal cells could be affected by exposure to thinner as it contains a vast mixture of volatile solvents that synergistically could have a direct effect on biology and viability of these cells. The present study aimed to test the toxicity of thinner on metabolic activities of neurons and astrocytes in primary cell culture. Methods: For the assessment of acute effects of thinner treatment on neuronal cells and the evaluation of the dose-and timeresponse dependencies, cultured neurons and astrocytes were treated acutely by gradually increased concentrations of thinner during different time of incubation. The MTT assay was used to estimate the cytotoxicity of the solvent. Results: Our results demonstrate that the thinner has an important cytotoxic effect and induces concentration-dependent cell death in both cultured neurons and astrocytes. In addition, the fact that the viability of glial cells is the only dependent on the exposure time demonstrated that the toxic effect causes a stronger degeneration over neurons than glial cells. Conclusions: The thinner has a cytotoxic effect on neurons and glial cells. We suggest that the wide range of neurological symptoms produced by inhalant exposure can be correlated with the direct effect of the solvent on the biology of neuronal cells.

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The Cytotoxicity of Some Organic Solvents on Isolated Hepatocytes in Monolayer Culture

Cited by 11 publications

References 10 publications

Combined treatment with quercetin and imperatorin as a potent strategy for killing HeLa and Hep-2 cells

Combined treatment with quercetin and imperatorin as a potent strategy for killing HeLa and Hep-2 cells

Cytotoxic Effects of Some Common Organic Solvents on MCF-7, RAW-264.7 and Human Umbilical Vein Endothelial Cells

Acute Paint Thinner Treatment Induces Cell Death in Cultured Neurons and Astrocytes

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