The Cytotoxicity of RoekoSeal and AH Plus Compared during Different Setting Periods

Abstract

The aim of this study was to evaluate the cytotoxicity of the root canal sealers RoekoSeal Automix (RSA) and AH Plus in vitro on human cervical carcinoma (HeLa) cells and mouse skin fibroblasts (L929). The sealers were covered with a 3 x 10(4) cell suspension 1 h, 24 h, 48 h, 7 days and 1 month after mixing. Four samples and respective controls without sealer were prepared. After 5 days of incubation, the number of cells was determined using an electronic counter; the number of viable cells was determined under light microscopy following the addition of nigrosin dye. The experiment was repeated twice for each sealer and each period. AH Plus was significantly more cytotoxic after 1 h, 24 h, and 48 h, compared to the 7 day and 1 month setting period on both cell lines. RoekoSeal had no cytotoxic effect on either cell line at any setting time.

Effect of time of extraction on the biocompatibility of endodontic sealers with primary human fibroblasts

“…8,18,24,25 Also, in accordance with Miletic et al 25 and Al-Hiyasat et al, 26 cytotoxicity was reduced after 7 days, while the present work also showed a decrease by 14, 21, and 28 days. In contrast, Azar et al, 27 using the neutral red assay, demonstrated that AH Plus induced significant cytotoxicity at 1 and 4 h, but no toxicity was detectable after 4 h. Huang et al 28 demonstrated that AH Plus at 24 h was cytotoxic to astrocytes in a dose-dependent manner.…”

“…This may be a problem commonly faced in studies with transformed cells, which are usually aneuploid. The expected desirable similarity of primary cell culture with in vivo tissue responses, and the recognition of human gingival fibroblasts as an adequate model for the early detection of possible cytotoxic effects of root canal filling materials, [16][17][18][19] led to the choice of these cells in the present methodology.…”

Cytotoxicity of root canal sealers on endothelial cell cultures

“…For this study, test specimens were prepared after handling sealers according to each manufacturer's instructions (11,14,22) and using devices (mold + extractor) specifically designed for the study and properly sterilized before use. For correct sterilization, test specimens were placed on a Petri plate and put in a microwave oven for two 5-min cycles at maximum output.…”

“…For correct sterilization, test specimens were placed on a Petri plate and put in a microwave oven for two 5-min cycles at maximum output. For surface disinfection, test specimens were exposed to ultraviolet light (14,25), for approximately 30 h. However, some authors (14) used a 2-h period whereas others (25) used a 24-h period. This type of incubation used in the present study to assess the effect of the sealers on ECV-304 cells has been used elsewhere (13,15,(23)(24)(25).…”

Avaliação In vitro da irradiação com laser de Nd:YAG e Er:YAG no selamento radicular, variando-se o cimento obturador do sistemas de canais radiculares

“…Seguindo a mesma linha de pesquisa, na qual adesão à estrutura dentária e selamento são diretamente proporcionais, desenvolveu-se também cimentos à base (BOUILLAGUET et al, 2006;ELDENIZ et al, 2007;ERSEV et al, 1999;LODIENE et al, 2008;GRECCA;FACHIN, 2009;SHIPPER et al, 2005;SOUSA et al, 2006 A toxicidade do cimento AH Plus™ foi considerada de severa (LODIENE et al, 2008;MERDAD et al, 2007;MILETIC et al, 2005;WILLERSHAUSEN et al, 2000) a leve ou inexistente (CAMPS; ABOUT, 2003;ELDENIZ et al, 2007;OZTAN et al, 2003;SCHWARZE et al, 2002;SCHWARZE;LEYHAUSEN;GEURTSEN, 2002), quando comparada a outros tipos de cimento. Da mesma forma, os cimentos Epiphany ® e EndoREZ ® foram considerados de muito a pouco citotóxicos na literatura (BOUILLAGUET et al, 2004;BOUILLAGUET et al, 2006;ELDENIZ et al, 2007;KEY;RAHEMTULLA;ELEAZER, 2006;LODIENE et al, 2008;MERDAD et al, 2007).…”