The Cytotoxic Effects of Camptothecin and Mastoparan on the Unicellular Green Alga Chlamydomonas reinhardtii

Abstract

We have recently reported that protease inhibitors affecting the activity of the proteasome cause necrotic cell death in Chlamydomonas reinhardtii instead of inducing apoptosis as shown for some mammalian cell lines. Therefore, we have studied other well-known inducers of apoptosis in mammalian cells for their effects on C. reinhardtii cells. Mastoparan caused rapid cell death without a prominent lag-phase under all growth conditions, whereas the cytotoxic effect of the topoisomerase I inhibitor camptothecin exclusively occurred during the cell-division phase. Essentially no differences between wall-deficient and wild-type cells were observed with respect to dose-response and time-course of camptothecin and mastoparan. In cultures of the wall-deficient strain, cell death was accompanied by swelling and subsequent disruption of the cells, established markers of necrosis. In case of the wild-type strain, camptothecin and mastoparan caused accumulation of apparently intact, but dead cells instead of cell debris due to the presence of the wall. Both in cultures of the wall-deficient and the wild-type strains, cell death was accompanied by an increase of the protein concentration in the culture medium indicating a lytic process like necrosis. Taking together, we have severe doubts on the existence of an apoptotic program in case of C. reinhardtii.

Hydrogen Sulfide Signaling Protects Chlamydomonas reinhardtii Against Allelopathic Damage From Cyanobacterial Toxin Microcystin-LR

“…Evans blue dye E2129 (Sigma, St. Louis, MO, USA) was used as a marker of cell death (Voigt et al, 2017). In brief, C. reinhardtii cells (45 ml, 10 6 cells/ml) were incubated with 0.1% (w/v) Evans blue for 5 min, then washed once with 50 ml of TAP medium, and re-suspended in an equal volume of TAP medium.…”