The cytotoxic effect of pinostrobin fingerroot (boesenbergia pandurata)on the culture of hela cells

Rosmelia, Rosmelia; Suryaningsih, Betty Ekawati; Anshory, Hady; Wirohadidjojo, Yohanes Widodo

doi:10.20885/jkki.vol7.iss4.art4

Cited by 2 publications

(2 citation statements)

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“…The IC 50 was determined, as the concentration required to inhibit 50% T47D cell growth and CC 50 was determined, as the concentration required to inhibit 50% Vero cells growth. 9 , 13…”

Section: Methodsmentioning

confidence: 99%

“…Previously, studies related to pinostrobin showed that its cytotoxic activity against cancer cells was still low compared to anticancer drugs on the market. 2 , 8 , 9 Then supported by the research of Jones & Gehler (2020), pinostrobin selectively decreases the spread of but has less effect on cancer cell death. Although a lot of research has been conducted related to pinostrobin, research related to the modification of its structure is still very rare, especially in the compound pinostrobin propionate and pinostrobin butyrate (Figure 1B,C).…”

Section: Introductionmentioning

confidence: 91%

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In vitro study of pinostrobin propionate and pinostrobin butyrate: Cytotoxic activity against breast cancer cell T47D and its selectivity index

Widiandani

Tandian

Zufar

et al. 2023

J. Public Health Afr.

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Backgrounds: Pinostrobin has the potential activity as an anti-cancer. However, its activity is still lower than the anticancer drugs on the market. To increase its activity, pinostrobin derivatives have been synthesized, namely pinostrobin propionate and pinostrobin butyrate, which are predicted to have better activity and lower toxicity than pinostrobin after being tested by in silico approach. So the compound deserves to be tested for its anticancer activity and selectivity on normal cells. Objective: This study aims to determine the anticancer activity of pinostrobin propionate and pinostrobin butyrate against the T47D breast cancer cell line and its selectivity against the Vero cell line. Methods: The cytotoxicity test which is anticancer activity test and its selectivity on normal cell were carried out using the MTT(3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay. The cells used were breast cancer cell line T47D and normal Vero cells. The test results were analyzed using a microplate reader with a wavelength of 570 nm. Results: From the analysis of anticancer activity on T47D cells, the IC50 values of pinostrobin, pinostrobin propionate, and pinostrobin butyrate were 2.93, 0.57, and 0.40 mM, respectively. While the results of the cytotoxicity test on Vero cells obtained the CC50 value of pinostrobin, pinostrobin propionate, pinostrobin butyrate was 1.27, 0.94, and 0.89 mM, respectively. So the SI value of pinostrobin (SI=0.4) is smaller than its derivatives (SI=1.7 and 2.2). Meanwhile, pinostrobin butyrate is more selective than pinostrobin propionate. Conclusions: It can be concluded that pinostrobin propionate and pinostrobin butyrate compounds have greater activity and selectivity than pinostrobin so these compounds are promising to be further developed as anticancer candidates.

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“…The IC 50 was determined, as the concentration required to inhibit 50% T47D cell growth and CC 50 was determined, as the concentration required to inhibit 50% Vero cells growth. 9 , 13…”

Section: Methodsmentioning

confidence: 99%