The Cytotoxic and Anti-proliferative Activity of High Molecular Weight Pectin and Modified Citrus Pectin

Abdel-Massih, Roula M.; Hawach, Venicia; Boujaoude, Marie-Anne

doi:10.31989/ffhd.v6i9.270

Cited by 8 publications

(6 citation statements)

References 36 publications

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“…Many previous studies have focused on Modified Citrus Pectin (MCP) as Gal-3 inhibitor with the ability of binding to Gal-3 as a ligand and claiming that due to limited solubility in water CP and AP in their naive form are unable to interact with Gal-3. However, the latest studies have reported that Pectin is a water-soluble dietary fiber 53 , and exhibit biological activities, ranging from anti-inflammatory, and anti-microbial activities to cardiovascular protective role 54 . A representative compound GCS-100, is a modified polysaccharide extracted from the peel and pulp of citrus fruits with high pH and temperature treatment, and is a Gal-3 antagonist that inhibits myeloma cell growth in vitro 55 .…”

Section: Discussionmentioning

confidence: 99%

Oxidative DNA damage induced by ROS-modulating agents with the ability to target DNA: A comparison of the biological characteristics of citrus pectin and apple pectin

Salehi

Behboudi

Kavoosi

2018

Sci Rep

139

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DNA targeting anticancer agents have been very successful in clinic, especially, when used in combinatorial therapy. But unfortunately, they often exhibit high levels of toxicity towards normal cells. Hence, much effort has been put into finding agents with more selectivity, and less toxicity. Pectins are natural polysaccharides, and beneficial nutritional fibers that have attracted attentions due to their antitumor properties. However, their molecular targets, and mechanism of action are widely unknown. Here, we have reported that citrus pectin (CP) and apple pectin (AP) selectively suppress viability in MDA-MB-231, MCF-7 and T47D human Breast cancer cells, while non-toxic to L929 normal cells. Upon CP, and AP treatments, cancer cells’ ROS content increased rapidly, and led to the collapse of the mitochondrial transmembrane potential which functions upstream of the caspase-dependent apoptosis. CP and AP treated cancer cells were also arrested at the S and G1 or G2/M phases of the cell cycle, respectively. Furthermore, mRNA expression of Galectin-3 (a multi-functional lectin involved in cell adhesion, cell cycle, and apoptosis) reduced in both CP and AP treated cells. Growth inhibition of MDA-MB-231 cells by CP, and AP was concomitant with DNA damage (oxidation, and strand breaks). In this context, in an effort to clarify the mechanism of action, we showed that CP, and AP are able to interact with DNA. The strength and mode of DNA binding were established by spectroscopy techniques. We demonstrated that CP, and AP bind to dsDNA by intercalation, and groove binding/partial intercalation, respectively. In conclusion, our findings suggest that CP, and AP induce apoptosis in MDA-MB-231 cells by increasing the release of ROS, which may be related to the mitochondrial apoptosis pathway, and direct interactions with DNA. Our data indicate that these compounds may be potentially useful in cancer treatment.

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Section: Discussionmentioning

confidence: 99%

Oxidative DNA damage induced by ROS-modulating agents with the ability to target DNA: A comparison of the biological characteristics of citrus pectin and apple pectin

Salehi

Behboudi

Kavoosi

2018

Sci Rep

139

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“…These findings remained almost ignored until the second decade of the 2000s, when a number of studies started to report a number of new pectin‐based substances with high bactericidal activity …”

Section: Figurementioning

confidence: 99%

“…In 2015, scholars in Lebanon reported the antibacterial activity of citrus pectin against S. aureus , a virulent pathogen which frequently contaminates food, including milk, cheese, meat, and dairy preparations . Pectin showed the highest antibacterial activity at pH 6 with minimum inhibitory concentration (MIC) values ranging from 0.39 mg mL −1 to 3.125 mg mL −1 …”

Section: Figurementioning

confidence: 99%

Superior Antibacterial Activity of Integral Lemon Pectin Extracted via Hydrodynamic Cavitation

Presentato

Scurria

Albanese³

et al. 2020

ChemistryOpen

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Pectin extracted via hydrodynamic cavitation in water only from waste lemon peel and further isolated via freeze drying displays significant antibacterial activity against Staphylococcus aureus, a Gram positive pathogen which easily contaminates food. The antibacterial effect of the new IntegroPectin is largely superior to that of commercial citrus pectin, opening the way to advanced applications of a new bioproduct now obtainable in large amounts and at low cost from citrus juice industry's waste.

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“…[37] A noticeable exception was the series of studies carried out by scholars in Lebanon reporting first the significant antibacterial activity of citrus pectin against Gram-negative H. pylori; [20] and then also against S. aureus and E. coli. [21] Using a new form of citrus pectin extracted and isolated from waste citrus peel under acid-free conditions by hydrodynamic cavitation in water followed by freeze drying,freezedrying scholars in Italy first confirmed the broad spectrum activity of pectin against both Gram-positive and Gram-negative ubiquitous pathogens such as S. aureus [33] and and P. aeruginosa. [34] Later, they extended the approach to grapefruit IntegroPectin providing the first insight on the antibacterial mechanism of this new form of pectic material interacting with the cell membrane of S. aureus and P. aeruginosa strains.…”

Section: Discussionmentioning

confidence: 99%

“…Two years later, the same team reported that optimal antibacterial activity against S. aureus and E. coli was observed at pH 6, with MIC values against S. aureus ranging between 0.39 mg/mL and 3.125 mg/mL and minimum bactericidal concentration (MBC) varying in the ample range 3.125–12.5 mg/mL [21] . A lower antibacterial activity was observed against E. coli with MICs of 25 and 50 mg/mL and MBC values ranging between 25 and 50 mg/mL.…”

Section: A Broad‐spectrum Antimicrobialmentioning

confidence: 99%

Pectin: A Long‐Neglected Broad‐Spectrum Antibacterial

Ciriminna

Fidalgo

Meneguzzo³

et al. 2020

ChemMedChem

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This article is dedicated to Professor Mohamed A. el-Nakeeb (University of Alexandria, Egypt) for his pioneering research on the antimicrobial activity of pectin First reported in the late 1930s and partly explained in 1970, the antibacterial activity of pectin remained almost ignored until the late 1990s. The concomitant emergence of research on natural antibacterials and new usages of pectin polysaccharides, including those in medicine widely researched in Russia, has led to a renaissance of research into the physiological properties of this uniquely versatile polysaccharide ubiquitous in plants and fruits. By collecting scattered information, this study provides an updated overview of the subtle factors affecting the behaviour of pectin as an antimicrobial. Less-degraded pectin extracted by acid-free routes, we argue in the conclusions, will soon find applications from new treatments for polymicrobial infections to use as an implantable biomaterial in tissue and bone engineering.

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The Cytotoxic and Anti-proliferative Activity of High Molecular Weight Pectin and Modified Citrus Pectin

Cited by 8 publications

References 36 publications

Oxidative DNA damage induced by ROS-modulating agents with the ability to target DNA: A comparison of the biological characteristics of citrus pectin and apple pectin

Oxidative DNA damage induced by ROS-modulating agents with the ability to target DNA: A comparison of the biological characteristics of citrus pectin and apple pectin

Superior Antibacterial Activity of Integral Lemon Pectin Extracted via Hydrodynamic Cavitation

Pectin: A Long‐Neglected Broad‐Spectrum Antibacterial

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