The Cytology of Rickettsiae

Ris, Hans; Fox, John P.

doi:10.1084/jem.89.6.681

Cited by 20 publications

(3 citation statements)

References 5 publications

(6 reference statements)

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“…Probably this dense structure corresl)onds to the deoxyribonucleic acid-containing body found in R. prowazekii by Ris and Fox (1949). In general, our morphological findings are in agreement with those of Stoker, Smith, and Fiset (1956), who examined thin sections of purified C. burnetii.…”

Section: Discussionsupporting

confidence: 90%

ELECTRON MICROSCOPIC OBSERVATIONS OF THE DEVELOPMENT OFCOXIELLA BURNETIIIN THE CHICK YOLK SAC

et al. 1964

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Section: Discussionsupporting

confidence: 90%

ELECTRON MICROSCOPIC OBSERVATIONS OF THE DEVELOPMENT OFCOXIELLA BURNETIIIN THE CHICK YOLK SAC

et al. 1964

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“…The presence of DNA in rickettsiae has been suggested by the histochemical studies of Ris and Fox (1949), who detected Feulgen-positive staining of R. prowazekii. Moreover, Smith and Stoker (1951) found that 9.7% of the dry weight of C. burnetii was DNA.…”

Section: Nmentioning

confidence: 95%

Fine Structure of Rickettsia quintana Cultivated In Vitro and in the Louse

Ito

Vinson

1965

J Bacteriol

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structure of Rickettsia quintana cultivated in vitro and in the louse. J. Bacteriol. 89: 481-495. 1965.-Usually rod-shaped, Rickettsia quintana cells measure about 0.2 to 0.5 , wide and up to 1.6 IA long. The rickettsiae have both an outer cell wall, about 80 A thick, and a plasma membrane, about 70 A thick, each of which is trilaminar. Occasional vesicular invaginations of the plasma membrane occur. The nuclear material, distributed in irregular zones throughout the cytoplasm, appears as a loose network of fine fibrils when postfixed with uranyl acetate and as thick strands or clumps after routine OS04 fixation. The cytoplasm is densely packed with numerous granules, presumably ribosomes, about 150 A in diameter. Histochemical studies revealed the presence of both ribonucleic and deoxyribonucleic acids. During binary fission, a constricting furrow is formed by the cell wall and plasmalemma. No difference in fine structure was observed between R. quintana propagated on cell-free media and in the louse. Rickettsiae constitute a grotul) of microorganisms regarded as intermediate between bacteria and viruses. Although their morphological and physiological characteristics more closely resemble those of certain bacteria, they depend, like viruses, on living host cells for their multiplication. With the exception of Rickettsia quintana, rickettsiae are obligatory intracellular parasites. R. quintana, the etiological agent of trench fever, multiplies extracellularly in the gut of its arthropod vector, Pediculus humanus var. corporis (Wolbach, Todd, and Palfrey, 1922); and, recently, Vinson and Fuller (1961) and Vinson, Varela, and Molina (in preparation) reported the cultivation of R. quintana on cell-free media, as well as in lice, in cells cultivated in vitro, and in the yolk sac of the developing chick embryo. Although studies on the fine structure of several rickettsiae have been reported by various investigators (

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“…(117)], for the observation of nuclei in fungus spores (8,13), and for the demonstration of chromatin bodies in Rickettsia (209). Observa tions of chromatin bodies in bacteria have also been made using the electron (102) and phase-contrast (244) microscopes.…”

Section: Mechanism Of Transmission and Genetic Recombinationmentioning

confidence: 99%