The cytokinesis-blocked micronucleus assay: Dose estimation and inter-individual differences in the response to γ-radiation

Antunes, Ana; Martins, Vanda; Cardoso, Júlio Roquete; Santos, Lia Raquel de Souza; Gil, Octávia Monteiro

doi:10.1016/j.mrgentox.2013.09.012

Cited by 20 publications

(6 citation statements)

References 46 publications

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“…Our results showed a signifi cant decrease in the NDI values of radiation workers compared with non-exposed individuals (p> 0.0005), that could be due to the chronic low dose exposure. Signifi cant decrease in the pooled NDI values with increasing radiation doses was also observed by other studies (Antunes et al, 2014, Pejchal et al, 2011, IAEA, 2001.…”

Section: Discussionsupporting

confidence: 82%

Low levels of ionizing radiation exposure and cytogenetic effects in radiopharmacists

Gharibdousty¹,

Zakeri²,

Changizi³

et al. 2017

Biosci. Biotech. Res. Comm

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Radiopharmaceuticals are unique medicinal formulations containing radioisotopes which are used in major clinical areas for diagnosis and/or therapy. The aim of this study was to assess occupationally induced DNA damage in workers of a radiopharmaceutical facility who are at risk of exposure to low levels of external ionizing radiation and internal contamination. Cytokinesis-block micronucleus (CBMN) assay used as an important biological indicator of ionizing radiation exposure in the peripheral blood lymphocytes of 35 exposed workers compared with 35 controls matched for gender, age and smoking habits. Occupational dosimetry records were collected over the last year (ranged from 1.05 to 30.73 mSv) and last 5 years exposure (ranged from 2.56 to 70.24 mSv). The results showed a 2.5 fold increase in the chromosomal damage leading to micronucleated lymphocytes in the workers of the radiopharmaceutical facility compared to the controls (25.82±8.67 vs. 10.52±6.83 micronuclei per 1000 binucleated cells, p < 0.0005). The nuclear division index as a parameter of cytostasis, in the workers was signifi cantly lower than that in the controls. The mean frequency of nucleoplasmic bridges was higher in the radiation workers compared to the controls without statistical signifi cant difference (p > 0.05). Taking all the confounding factors into account, no obvious trend of increased micronuclei as a function of either duration of employment, exposed dose, smoking or age was observed. The present study showed that occupationally exposed individuals have higher frequencies of DNA damage, despite the very low levels of ionizing radiation exposure.

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Section: Discussionsupporting

confidence: 82%

Low levels of ionizing radiation exposure and cytogenetic effects in radiopharmacists

Gharibdousty¹,

Zakeri²,

Changizi³

et al. 2017

Biosci. Biotech. Res. Comm

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“…6,10 Earlier studies have documented the existence of interindividual variations in the yield of chromosomal aberrations induced by ionizing radiation and chemicals. 21,22 However, these variations do not seem to be associated with either age or lifestyle indicating that other confounding factors may be responsible for these variations. Since humans have different blood types, we investigated whether or not blood types influence the proliferative potential of lymphocytes.…”

Section: Discussionmentioning

confidence: 94%

“…Since optimal lymphocyte proliferation is pivotal for cytogenetic assessment of cytotoxic and genotoxic exposures, identification of confounding factors or experimental variables is critical for its use in human biomonitoring . Earlier studies have documented the existence of interindividual variations in the yield of chromosomal aberrations induced by ionizing radiation and chemicals . However, these variations do not seem to be associated with either age or lifestyle indicating that other confounding factors may be responsible for these variations.…”

Section: Discussionmentioning

confidence: 99%

Does proliferation capacity of lymphocytes depend on human blood types?

Viswanathan

Kanagaraj

Raavi

et al. 2018

J of Cellular Biochemistry

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In vitro human lymphocyte culture methodology is well established yet certain confounding factors such as age, medical history as well as individual’s blood type may potentially modulate in vitro proliferation response. These factors have to be carefully evaluated to release reliable test report in routine cytogenetic evaluation for various genetic conditions, radiation biodosimetry, etc. With this objective, the current study was focused on analyzing the proliferation response of lymphocytes drawn from 90 individuals (21‐29 years) with different blood types. The proliferation response was assessed in the cultured lymphocytes by cell cycle, mitotic index (MI), and nuclear division index (NDI) after stimulation with phytohaemagglutinin (PHA). To investigate the toxic effect on proliferation, MI was calculated in representative samples of each blood type were X‐irradiated. The results showed that there was no significant difference among the cell cycle phases of lymphocytes in different blood types (P > 0.05). Similarly, both MI and NDI of lymphocytes derived from different blood types also did not show significant difference ( P > 0.05). The extensive interindividual variation within and among the blood types is likely responsible for the lack of significant difference in lymphocyte proliferation. Although spontaneous proliferation efficiency of lymphocytes of different blood types after PHA stimulation was grossly similar, the MI observed after radiation exposure showed a significant difference ( P < 0.05) indicating a differential proliferation response among the blood types. Our results suggest that the blood types did not have any impact on PHA‐induced proliferation; however, a specific differential lymphocyte proliferation observed after radiation exposure needs to be considered.

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“…Na tabela 2 são comparados os valores do teste u de outros trabalhos [14][15][16][17] De acordo com Romm et al (2013), as curvas de calibração dose-resposta de MN apresentam os coeficientes lineares (α) com valores maiores, ou seja, mais significativo na estimativa de dose, do que os coeficientes quadráticos (β), logo interação única da radiação tem a maior contribuição na formação de MN, mesmo mudando a qualidade da radiação, a forma de análise (manual ou automática) e o total de células analisadas [18].…”

Section: Resultsunclassified

Análise estatística da frequência de micronúcleos em diferentes doses absorvidas de raios gama

silva

Silva²,

Nascimento³

et al. 2020

Braz. J. Rad. Sci.

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A técnica de micronúcleos (MN) com bloqueio da citocinese celular (CBMN -Cytokinesis-Block Micronucleus) é uma técnica da biodosimetria que, por ser mais rápida na análise que a técnica de dicêntricos, possibilita realizar triagens entre indivíduos expostos significativamente ou não à radiação ionizante em casos de acidentes radiológicos em massa. Entretanto, para isso é necessário que curvas de calibração dose-resposta sejam previamente estabelecidas pelo laboratório que se propuser a realizar serviços de dosimetria biológica. O objetivo desse trabalho é analisar as frequências de MN devido à irradiação de sangue in vitro com raios gama para compor a curva de calibração dose-resposta do Laboratório de Dosimetria Biológica (LDB) do Centro Regional de Ciências Nucleares do Nordeste (CRCN-NE/CNEN). As amostras foram irradiadas de forma a resultar em valores de doses absorvidas de 0,5Gy a 2,0Gy. Após a irradiação, as amostras passaram duas horas em estufa de CO2 para reparação das células, depois seguiram para a cultura de linfócitos conforme protocolo descrito pela IAEA. As análises estatísticas foram realizadas por meio do teste u de Papworth a fim de verificar se a distribuição de células com MN se ajustam ao modelo de Poisson. As frequências de micronúcleo, bem como sua distribuição, estão se apresentando de acordo com o esperado e serão utilizados na curva de calibração doseresposta de MN para radiação gama do LDB/CRCN-NE/CNEN. Os experimentos serão replicados com outras doses e outros voluntários, tornando a curva mais robusta e viabilizando a comparação da resposta nas células de indivíduos em diferentes faixa etárias e gêneros.

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The cytokinesis-blocked micronucleus assay: Dose estimation and inter-individual differences in the response to γ-radiation

Cited by 20 publications

References 46 publications

Low levels of ionizing radiation exposure and cytogenetic effects in radiopharmacists

Low levels of ionizing radiation exposure and cytogenetic effects in radiopharmacists

Does proliferation capacity of lymphocytes depend on human blood types?

Análise estatística da frequência de micronúcleos em diferentes doses absorvidas de raios gama

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