The cytochrome P450 family in the parasitic nematode Haemonchus contortus

Laing, Roz; Bartley, David J.; Morrison, Alison A.; Rezansoff, Andrew M.; Martinelli, Axel; Laing, Steven T.; Gilleard, John S.

doi:10.1016/j.ijpara.2014.12.001

Cited by 42 publications

(41 citation statements)

References 60 publications

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“…However, this view has been changed with the discovery of CYPs-encoding genes in C. elegans, where more than 80 CYP proteins are present [85,86], and these have been shown to be xenobiotics inducible [85,[87][88][89][90]. The CYPs family have also been characterized recently in H. contortus [91,92]. ABC transporter and pgp fall under the phase III of xenobiotic detoxification process.…”

Section: Discussionmentioning

confidence: 99%

A Whole Genome Re-Sequencing Based GWA Analysis Reveals Candidate Genes Associated with Ivermectin Resistance in Haemonchus contortus

Khan

Nisar

Yuan

et al. 2020

Genes

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The most important and broad-spectrum drug used to control the parasitic worms to date is ivermectin (IVM). Resistance against IVM has emerged in parasites, and preserving its efficacy is now becoming a serious issue. The parasitic nematode Haemonchus contortus (Rudolphi, 1803) is economically an important parasite of small ruminants across the globe, which has a successful track record in IVM resistance. There are growing evidences regarding the multigenic nature of IVM resistance, and although some genes have been proposed as candidates of IVM resistance using lower magnification of genome, the genetic basis of IVM resistance still remains poorly resolved. Using the full magnification of genome, we herein applied a population genomics approach to characterize genome-wide signatures of selection among pooled worms from two susceptible and six ivermectin-resistant isolates of H. contortus, and revealed candidate genes under selection in relation to IVM resistance. These candidates also included a previously known IVM-resistance-associated candidate gene HCON_00148840, glc-3. Finally, an RNA-interference-based functional validation assay revealed the HCON_00143950 as IVM-tolerance-associated gene in H. contortus. The possible role of this gene in IVM resistance could be detoxification of xenobiotic in phase I of xenobiotic metabolism. The results of this study further enhance our understanding on the IVM resistance and continue to provide further evidence in favor of multigenic nature of IVM resistance.

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Section: Discussionmentioning

confidence: 99%

A Whole Genome Re-Sequencing Based GWA Analysis Reveals Candidate Genes Associated with Ivermectin Resistance in Haemonchus contortus

Khan

Nisar

Yuan

et al. 2020

Genes

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“…In addition, a positively responding pattern of three known cytochrome P450 genes from PWN was further proved by qRT‐PCR. Cytochrome P450 was widely considered as related to the detoxification [36–38], development [39–41] and life span regulation function [42–44]. Elevated expression levels of the three cytochrome P450 genes would be not only a cellular defense mechanism to avoid PWNs from being damaged by low temperature, but also a cellular signal cue for PWNs to enter diapause stage.…”

Section: Discussionmentioning

confidence: 99%

Cytochrome P450 metabolism mediates low‐temperature resistance in pinewood nematode

Wang

Hao

et al. 2020

FEBS Open Bio

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Pinewood nematode (PWN; Bursaphelenchus xylophilus) is a devastating invasive species that is expanding into colder regions. Here, we investigated the molecular mechanisms underlying low‐temperature resistance of PWN. We identified differentially expressed genes enriched under low temperature in previously published transcriptome data using the Kyoto Encyclopedia of Genes and Genomes. Quantitative real‐time PCR was used to further validate the transcript level changes of three known cytochrome P450 genes under low temperature. RNA interference was used to validate the low‐temperature resistance function of three cytochrome P450 genes from PWN. We report that differentially expressed genes were significantly enriched in two cytochrome P450‐related pathways under low‐temperature treatment. Heatmap visualization of transcript levels of cytochrome P450‐related genes revealed widely different transcript patterns between PWNs treated under low and regular temperatures. Transcript levels of three cytochrome P450 genes from PWNs were elevated at low temperature, and knockdown of these genes decreased the survival rates of PWNs under low temperature. In summary, these findings suggest that cytochrome P450 metabolism plays a critical role in the low‐temperature resistance mechanism of PWN.

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“…Furthermore, E. granulosus and T. solium are also able to use detoxification enzymes, such as thioredoxin, glutathione peroxidase, or thioredoxin glutathione reductase, against organic compounds (Tsai et al 2013;Wang et al 2018). Additionally, CYP oxidase presence has been demonstrated in some parasitic nematodes (Kotze 1997;Cvilink et al 2008;Laing et al 2015). For example, the genome of Caenorhabditis elegans encodes 80 CYPs (Menzel et al 2001(Menzel et al , 2005.…”

Section: Discussionmentioning

confidence: 99%

Polycyclic aromatic hydrocarbons in the cestode Oncomegas wageneri parasite of Mexican flounder Cyclopsetta chittendeni

et al. 2020

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The concentrations of polycyclic aromatic hydrocarbon metabolites (PAHm) and their bioconcentration factors (BCF) were determined in the larval stages of the cestode Oncomegas wageneri, recovered from the intestine of the Mexican flounder Cyclopsetta chittendeni, in the southern Gulf of Mexico. The PAHm concentrations in O. wageneri were measured using fixed-wavelength fluorescence spectrometry and compared with PAHm concentrations in host bile. Oncomegas wageneri PAHm concentrations were markedly higher than those in host tissues. The highest BCF values were obtained for 1hydroxypyrene (OHP) and benzo(a)pyrene (BaP). Using a General Linear Model, a significant negative relationship was found between O. wageneri PAHm concentrations (as response variable) and the number of O. wageneri and oil well proximity. Low BCF values and PAHm concentrations in C. chittendeni correlated positively with O. wageneri PAHm concentrations. In contrast, high BCF values for PAHm concentrations in C. chittendeni had a negative association with O. wageneri PAHm concentrations. This study provides the first evidence of the presence of PAHm in intestinal larval cestodes of marine flatfishes, demonstrating levels of PAHm that were higher than levels in their hosts.

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The cytochrome P450 family in the parasitic nematode Haemonchus contortus

Cited by 42 publications

References 60 publications

A Whole Genome Re-Sequencing Based GWA Analysis Reveals Candidate Genes Associated with Ivermectin Resistance in Haemonchus contortus

A Whole Genome Re-Sequencing Based GWA Analysis Reveals Candidate Genes Associated with Ivermectin Resistance in Haemonchus contortus

Cytochrome P450 metabolism mediates low‐temperature resistance in pinewood nematode

Polycyclic aromatic hydrocarbons in the cestode Oncomegas wageneri parasite of Mexican flounder Cyclopsetta chittendeni

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