The Cytochrome cbo from the Obligate Methylotroph Methylobacillus flagellatus KT Is a Cytochrome c Oxidase

Strom, E. V.; Dinarieva, T. Yu.; Netrusov, A. I.

doi:10.1023/b:mici.0000023976.80874.ef

Cited by 3 publications

(3 citation statements)

References 18 publications

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“…Strains of hydrocarbon-oxidizing microorganisms were isolated from samples of oilcontaminated arable soils from the territory of the Republic of Bashkortostan, where various herbicides were used. We used the method of enrichment cultures [51]. A 100 mL measure of liquid Raymond mineral medium (composition (g/L): NH 4 NO 3 -2.0, MgSO 4 × 7H 2 O-0.2, KH 2 PO 4 -2.0, Na 2 HPO 4 -3.0, CaCl 2 × 6H 2 O-0.01, Na 2 CO 3 -0.1, pH-7.0) [52] was introduced into flasks, and 1% (w/v) oil (the only source of carbon and energy) and 2 g of soil were added.…”

Section: Isolation Of Hydrocarbon-degrading Strainsmentioning

confidence: 99%

“…The production of hydrolytic enzymes by the strains was determined by the following methods: protease-by liquefaction of gelatin, amylase-by the diameter of the starch hydrolysis zone, cellulase-by the presence of a zone of dissolution of carboxymethylcellulose, and lipases-by the presence of an opaque zone of calcium salts of fatty acids on a medium with Tween 80 [51].…”

Section: Production Of Hydrolytic Enzymes Of Strainsmentioning

confidence: 99%

“…The ability of the strains to fixate nitrogen was determined by growth rates on Ashby agar medium (composition (g/L): mannitol-20.0, K 2 HPO 4 -0.2, MgSO 4 ×7H 2 O-0.2, NaCl-0.2, K 2 SO 4 -0.1, CaCO 3 -5.0, agar-agar-15.0) [51] after 5 days of cultivation at 28 • C. The strains were considered active if the number of their cells increased from 10 5 to 10 9 CFU/mL or more during 72 h of cultivation. A lower growth rate was interpreted as weak growth.…”

Section: Nitrogen Fixationmentioning

confidence: 99%

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Promising Strains of Hydrocarbon-Oxidizing Pseudomonads with Herbicide Resistance and Plant Growth-Stimulating Properties for Bioremediation of Oil-Contaminated Agricultural Soils

Korshunova,

Kuzina,

Mukhamatdyarova

et al. 2023

Agriculture

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Nowadays, large areas of agricultural land are contaminated with chemical plant-protection products. Agricultural soils are also susceptible to oil pollution as a result of accidents on oil pipelines. Bioremediation of such soils from oil with the help of hydrocarbon-oxidizing bacteria is hindered by the presence of additional pollutants such as herbicides. In this work, seven strains of Pseudomonas were isolated and identified, which showed differences in ability of oil biodegradation (32.7–77.3%). All strains showed resistance to herbicides based on 2,4-D and substances from the class of imidazolinones, possessed phosphate-solubilizing and nitrogen-fixing activity, and produced indolyl-3-acetic acid (305–1627 ng/mL culture liquid). They stimulated the growth of barley and clover in soil with oil, as well as the growth of clover in soil with herbicide. In a vegetative experiment (duration 30 days, initial oil content in soil 2% wt., herbicide based on imazethapyr 0.002% wt.) of barley plants and P. alcaligenes UOM 10 or P. frederiksbergensis UOM 11, oil degradation was 48.1–52.7%, the same strains and clover plants, 37.9–38.6%. The studied bacteria have the potential to be used in the bioremediation of oil-contaminated agricultural soils, including in combination with phytomeliorant plants.

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Section: Isolation Of Hydrocarbon-degrading Strainsmentioning

confidence: 99%

Section: Production Of Hydrolytic Enzymes Of Strainsmentioning

confidence: 99%

Section: Nitrogen Fixationmentioning

confidence: 99%

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Promising Strains of Hydrocarbon-Oxidizing Pseudomonads with Herbicide Resistance and Plant Growth-Stimulating Properties for Bioremediation of Oil-Contaminated Agricultural Soils

Korshunova,

Kuzina,

Mukhamatdyarova

et al. 2023

Agriculture

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Ferrous iron oxidation in moderately thermophilic acidophileSulfobacillus sibiricusN1^T

et al. 2010

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The iron-oxidizing system of a moderately thermophilic, extremely acidophilic, gram-positive mixotroph, Sulfobacillus sibiricus N1(T), was studied by spectroscopic, high-performance liquid chromatography and inhibitory analyses. Hemes B, A, and O were detected in membranes of S. sibiricus N1(T). It is proposed that the electron transport chain from Fe²(+) to O₂ is terminated by 2 physiological oxidases: aa₃-type cytochrome, which dominates in the early-exponential phase of growth, and bo₃-type cytochrome, whose role in iron oxidation becomes more prominent upon growth of the culture. Both oxidases were sensitive to cyanide and azide. Cytochrome aa₃ was more sensitive to cyanide and azide, with K(i) values of 4.1 and 2.5 µmol·L⁻¹, respectively, compared with K(i) values for cytochrome bo₃, which were 9.5 µmol·L⁻¹ for cyanide and 7.0 µmol·L⁻¹ for azide. This is the first evidence for the participation of a bo₃-type oxidase in ferrous iron oxidation. The respiratory chain of the mixotroph contains, in addition to the 2 terminal oxidases, a membrane-bound cytochrome b₅₇₃.

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Purification and characterization of azurin from the methylamine-utilizing obligate methylotrophMethylobacillus flagellatusKT

et al. 2012

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Methylamine dehydrogenase (MADH) and azurin were purified from the periplasmic fraction of the methylamine-grown obligate methylotroph Methylobacillus flagellatus KT. The molecular mass of the purified azurin was 16.3 kDa, as measured by SDS-PAGE, or 13 920 Da as determined by MALDI-TOF mass spectrometry. Azurin of M. flagellatus KT contained 1 copper atom per molecule and had an absorption maximum at 620 nm in the oxidized state. The redox potential of azurin measured at pH 7.0 by square-wave voltammetry was +275 mV versus normal hydrogen electrode. MADH reduced azurin in the presence of methylamine, indicating that this cupredoxin is likely to be the physiological electron acceptor for MADH in the electron transport chain of the methylotroph. A scheme of electron transport functioning in M. flagellatus KТ during methylamine oxidation is proposed.

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The Cytochrome cbo from the Obligate Methylotroph Methylobacillus flagellatus KT Is a Cytochrome c Oxidase

Cited by 3 publications

References 18 publications

Promising Strains of Hydrocarbon-Oxidizing Pseudomonads with Herbicide Resistance and Plant Growth-Stimulating Properties for Bioremediation of Oil-Contaminated Agricultural Soils

Promising Strains of Hydrocarbon-Oxidizing Pseudomonads with Herbicide Resistance and Plant Growth-Stimulating Properties for Bioremediation of Oil-Contaminated Agricultural Soils

Ferrous iron oxidation in moderately thermophilic acidophileSulfobacillus sibiricusN1^T

Purification and characterization of azurin from the methylamine-utilizing obligate methylotrophMethylobacillus flagellatusKT

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The Cytochrome cbo from the Obligate Methylotroph Methylobacillus flagellatus KT Is a Cytochrome c Oxidase

Cited by 3 publications

References 18 publications

Promising Strains of Hydrocarbon-Oxidizing Pseudomonads with Herbicide Resistance and Plant Growth-Stimulating Properties for Bioremediation of Oil-Contaminated Agricultural Soils

Promising Strains of Hydrocarbon-Oxidizing Pseudomonads with Herbicide Resistance and Plant Growth-Stimulating Properties for Bioremediation of Oil-Contaminated Agricultural Soils

Ferrous iron oxidation in moderately thermophilic acidophileSulfobacillus sibiricusN1T

Purification and characterization of azurin from the methylamine-utilizing obligate methylotrophMethylobacillus flagellatusKT

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Ferrous iron oxidation in moderately thermophilic acidophileSulfobacillus sibiricusN1^T