The cys-loop ligand-gated ion channel gene superfamily of the parasitoid wasp, Nasonia vitripennis

Jones, Andrew K.; Bera, Agata N.; Lees, Kristin; Sattelle, David B.

doi:10.1038/hdy.2009.97

Cited by 65 publications

(72 citation statements)

References 58 publications

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“…1A). The equivalent exon is (Kita et al, 2014), whereas only two are found in D. melanogaster (Semenov and Pak, 1999) and Nasonia vitripennis (Jones et al, 2010). The variant we observed in this work in which exon 3 is omitted (Fig.…”

Section: Discussionmentioning

confidence: 37%

“…In contrast, a single GluCl subunit was isolated from Drosophila melanogaster, which generates an IVM-sensitive channel when expressed in X. laevis oocytes (Cully et al, 1996). Subsequent analysis of genome sequences has shown that several insect species possess only a single glucl gene Sattelle, 2006, 2007;Jones et al, 2010). Even a single gene can yield diverse receptor subtypes.…”

Section: Introductionmentioning

confidence: 99%

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Exon 3 Splicing and Mutagenesis Identify Residues Influencing Cell Surface Density of Heterologously Expressed Silkworm (Bombyx mori) Glutamate-Gated Chloride Channels

et al. 2014

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Glutamate-gated chloride channels (GluCls) mediate fast inhibitory neurotransmission in invertebrate nervous systems. Insect GluCls show alternative splicing, and, to determine its impact on channel function and pharmacology, we isolated GluCl cDNAs from larvae of the silkworm (Bombyx mori). We show that six B. mori glutamategated chloride channel variants are generated by splicing in exons 3 and 9 and that exons 3b and 3c are common in the brain and third thoracic ganglion. When expressed in Xenopus laevis oocytes, the three functional exon 3 variants (3a, b, c) all had similar EC 50 values for L-glutamate and ivermectin (IVM); however, I max (the maximum L-glutamate-and IVM-induced response of the channels at saturating concentrations) differed strikingly between variants, with the 3c variant showing the largest L-glutamate-and IVM-induced responses. By contrast, a partial deletion detected in exon 9 had a much smaller impact on L-glutamate and IVM actions. Binding assays using [ Modeling the GluCl 3a and 3c variants suggested that three of the four amino acids contribute to intersubunit contacts, whereas the other interacts with the TM2-TM3 linker, influencing the receptor response.

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Section: Discussionmentioning

confidence: 37%

Section: Introductionmentioning

confidence: 99%

Exon 3 Splicing and Mutagenesis Identify Residues Influencing Cell Surface Density of Heterologously Expressed Silkworm (Bombyx mori) Glutamate-Gated Chloride Channels

et al. 2014

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“…In Drosophila, pHCl-2 groups with two other orphan pLGIC subunits, CG7589 and CG6927 (Dent, 2006;Jones and Sattelle, 2006;Remnant et al, 2016), which together most closely resemble the Drosophila pH-sensitive chloride channel ( pHCl) and the pHsensitive chloride channel in S. scabiei (SsCl) (Mounsey et al, 2007;Schnizler et al, 2005). Clades of channel subunits orthologous to the clade of subunits defined by pHCl-2, CG7589 and CG6927 have been reported in other insects such as Apis mellifera, A. aegypti, Nasonia vitripennis and Tribolium cataneums Sattelle, 2006, 2007;Jones et al, 2010;Remnant et al, 2016; www.flybase.org) and in non-insect arthropods such as the deer tick Ixodes scapularis (www.flybase.org), but not in nematodes, molluscs, annelids or chordates (Dent, 2006). The pH response of pHCl-2 closely resembles that of the two other characterized pH-sensitive arthropod pLGICs, Drosophila pHCl and Sarcoptes SsCl; both pHCl and SsCl are inhibited by protons and are increasingly activated by a rise in alkalinity, exhibiting halfmaximal activity at pH 7.33±0.16 and 7.55±0.06, respectively (Mounsey et al, 2007;Schnizler et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

The orphan pentameric ligand-gated ion channelpHCl-2is gated by pH and regulates fluid secretion inDrosophilaMalpighian tubules

Feingold

Starc

O’Donnell

et al. 2016

Journal of Experimental Biology

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Pentameric ligand-gated ion channels ( pLGICs) constitute a large protein superfamily in metazoa whose role as neurotransmitter receptors mediating rapid, ionotropic synaptic transmission has been extensively studied. Although the vast majority of pLGICs appear to be neurotransmitter receptors, the identification of pLGICs in non-neuronal tissues and homologous pLGIC-like proteins in prokaryotes points to biological functions, possibly ancestral, that are independent of neuronal signalling. Here, we report the molecular and physiological characterization of a highly divergent, orphan pLGIC subunit encoded by the pHCl-2 (CG11340) gene, in Drosophila melanogaster. We show that pHCl-2 forms a channel that is insensitive to a wide array of neurotransmitters, but is instead gated by changes in extracellular pH. pHCl-2 is expressed in the Malpighian tubules, which are non-innervated renal-type secretory tissues. We demonstrate that pHCl-2 is localized to the apical membrane of the epithelial principal cells of the tubules and that loss of pHCl-2 reduces urine production during diuresis. Our data implicate pHCl-2 as an important source of chloride conductance required for proper urine production, highlighting a novel role for pLGICs in epithelial tissues regulating fluid secretion and osmotic homeostasis.

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“…These studies have been extended to other nematode species, especially the animal and human parasites, and have revealed that the size and composition of the GluCl family vary between species, with avr-14 and glc-2 orthologs present in all genomes studied to date (32)(33)(34). A single GluCl gene, GluCl␣, is present in most insects, although its transcripts are extensively modified by mRNA splicing and editing (35)(36)(37), along with two genes encoding HisCl subunits and a single pHCl gene (36 -38). In Drosophila melanogaster, GluCl␣ mediates sensitivity to ivermectin and nodulisporic acid (18,39), suggesting that the avermectin drug target is the same throughout the Ecdysozoa.…”

Section: Exploitation Of Gluclsmentioning

confidence: 99%

Glutamate-gated Chloride Channels

Wolstenholme

2012

Journal of Biological Chemistry

211

174

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Glutamate-gated chloride channels (GluCls) are found only in protostome invertebrate phyla but are closely related to mammalian glycine receptors. They have a number of roles in these animals, controlling locomotion and feeding and mediating sensory inputs into behavior. In nematodes and arthropods, they are targeted by the macrocyclic lactone family of anthelmintics and pesticides, making the GluCls of considerable medical and economic importance. Recently, the three-dimensional structure of a GluCl was solved, the first for any eukaryotic ligandgated anion channel, revealing a macrocyclic lactone-binding site between the channel domains of adjacent subunits. This minireview will highlight some unique features of the GluCls and illustrate their contribution to our knowledge of the entire Cys loop ligand-gated ion channel superfamily.

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The cys-loop ligand-gated ion channel gene superfamily of the parasitoid wasp, Nasonia vitripennis

Cited by 65 publications

References 58 publications

Exon 3 Splicing and Mutagenesis Identify Residues Influencing Cell Surface Density of Heterologously Expressed Silkworm (Bombyx mori) Glutamate-Gated Chloride Channels

Exon 3 Splicing and Mutagenesis Identify Residues Influencing Cell Surface Density of Heterologously Expressed Silkworm (Bombyx mori) Glutamate-Gated Chloride Channels

The orphan pentameric ligand-gated ion channelpHCl-2is gated by pH and regulates fluid secretion inDrosophilaMalpighian tubules

Glutamate-gated Chloride Channels

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