The cutting-grinding technique for histologic preparation of undecalcified bone and bone-anchored implants

Informed choices for challenging specimens when choosing methacrylate resin systems for histology

“…However, historically, the resin protocol was developed for preparing sections from fully mineralized tissues with and without medical prostheses. Other specimens may include different types of tissue engineering devices including collagen membrane, polyethylene terephthalate felts (Singhrao et al, unpublished data), ceramics, dental composite material, and cardiovascular stents (Donath, 1988(Donath, , 1993Donath and Breuner, 1982;Gross and Strunz, 1977;Malik et al, 1998;Peterson et al, 1979;Plenk, 1986;Rammelt et al, 2007;Reck and Diekelmann, 1980;Rohrer and Schubert, 1992). The system is also suitable for embedding tiny cell culture pellets to maximize tissue availability for stem cell-based therapies (Williams et al, 2010).…”

“…The original sawing and grinding techniques, especially of tissues such as unsupported teeth, suffered from poor quality preservation of the associated soft tissues. The developmental research was pioneered by the demand to retain cellular details at intermediate magnification similar to that produced using light microscopy (Donath, 1993;Donath and Breuner, 1982;Gross and Strunz, 1977;Klawitter and Hulbert, 1971;Malik et al, 1998;Peterson et al, 1979;Plenk, 1986;Reck and Diekelmann, 1980;Rohrer and Schubert, 1992). At the time of inception (mid 1960s), only radiography analysis was available for examining such difficult specimens.…”

“…Depending on the hardness of the tissue sample, the cutting-grinding apparatus with a diamondcoated saw band produces thick slices (up to mm thickness (Rohrer and Schubert, 1992)). The samples are subsequently ground to or below 10 lm thickness to facilitate histological staining and cyto-architectural evaluation by light microscopy.…”

Reevaluation of the Strain Pressure Theory: Toward a Better Understanding the Biology of Tooth Movement

“…But those answers were not based on evidence practice models, thus interfering with the proper understanding of the mechanical principles dental movement acceleration. Using 5 a 10 µ width histological slice (Steigman et al, 1987;Rohrer & Schubert, 1992) limits microscopic observations to an extremely limited 2 dimension area compared to a real life 3D experience, dynamic as the cascade of cellular and molecular interactions that are involved during the application of an orthodontic force. Histological findings are not up for discussion, but the use of an animal model is; the animal model used, the time in which the animal was put away after force applied and for how long the force was applied.…”

Bone Sectioning Using the Exakt System

“…2 Further development of instruments that were specifically designed for this method by Biermann (Exakt, Hamburg-Norderstedt, Germany) beginning in 1983 has made it possible for many laboratories throughout the world to easily perform hard-tissue histotechnology. 6,8,9 In some centers of dental research sections of mineralized bones or teeth had already been used for histologic studies. 1,10 However, this routine method of sectioning non-demineralized enamel-free teeth or bones was not free of artifacts and could not be used for teeth with enamel, teeth with metallic fillings, crowns or bridges, or bones with ceramic or metallic implants.…”