The cuticular proteins of Hyalophora cecropia from different anatomical regions and metamorphic stages

Cox, Diana L.; Willis, Judith H.

doi:10.1016/0020-1790(85)90026-5

Cited by 97 publications

(30 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These particular mRNAs were undetectable or present in trace amounts during pupal or adult cuticle deposition. Whether these genes are expressed in other regions than the dorsal abdomen in the pupal or adult stage, as is true for several larval cuticular proteins of HyuZophoru cecropia [22,23], has not yet been determined.…”

Section: Discussionmentioning

confidence: 96%

Hormonal regulation of sequential larval cuticular gene expression

Riddiford

1986

Arch. Insect Biochem. Physiol.

View full text Add to dashboard Cite

As the tobacco hornworm (Manduca sexta) larva feeds and grows, the abdominal epidermis synthesizes larval endocuticular proteins. During a molt this synthesis temporarily ceases while the cells deposit a new epicuticle; then these proteins reappear in a sequential manner, some before ecdysis, others after ecdysis. At the time of metamorphosis, the epidermis becomes pupally committed and ceases synthesizing these endocuticular proteins. Northern and dot blot hybridization analysis using cDNA clones for three different low-molecular-weight larval cuticular proteins showed that RNA transcription of all three ceased by the time of head cap slippage during the molt, then begins again just before ecdysis, one showing a different time course from the other two. All responded to the commitment peak of ecdysteroid in the absence of juvenile hormone OH) by a permanent cessation of transcription.During the final day of feeding (day 3) of the 5th instar the cuticular lamellae become 5-10 times thinner coincidentally with a change in cuticular protein synthesis involving a cessation of synthesis of many of the endocuticular proteins and the appearance of at least three new proteins -14.5,15, and 27 kd (kilodaltons). Incubation of day 1 5th instar epidermis with 10-100 ng/ml 20-hydroxyecdysone (20HE) induced the synthesis of the 27-kd protein and the transcription of the mRNAs for the 14.5-and 15-kd proteins. Incubation in the absence of hormones or with 20HE in the presence of JH prevented the appearance of these new proteins and the deposition of thin lamellae. The mRNAs for three endocuticular proteins whose synthesis ceases on day 3 showed differing responses to 20HE and JH. Thus, the sequential program of larval endocuticular gene expression is controlled primarily by hormonal titers in both the molt and the intermolt periods.

show abstract

Section: Discussionmentioning

confidence: 96%

Hormonal regulation of sequential larval cuticular gene expression

Riddiford

1986

Arch. Insect Biochem. Physiol.

View full text Add to dashboard Cite

show abstract

“…Although it is generally believed that CPs expressed at different developmental stages or in different body regions assemble a cuticle with appropriate mechanical properties such as rigidity or flexibility (Cox and Willis, 1985;Willis et al, 2005), the precise location of CPs within a cuticle is still not well determined. Very recently, Vannini et al (2014) analyzed the expression of AgamCPF3 and AgamCPLCG3/4 proteins in A. gambiae and localized them using electron microscopic immunocytochemistry.…”

Section: Introductionmentioning

confidence: 99%

Two major cuticular proteins are required for assembly of horizontal laminae and vertical pore canals in rigid cuticle of Tribolium castaneum

Noh

Kramer

Muthukrishnan

et al. 2014

Insect Biochemistry and Molecular Biology

View full text Add to dashboard Cite

“…Electrophoretic studies and amino-acid analyses have demonstrated the existence in each type of cuticle of a rather large number of proteins (Roberts and Willis, 1980;Hojrup et al, 1986). Some of them are shared in different regions or developmental stages, while others have a much more restricted pattern of expression and thus contribute to the architectural and functional diversity of cuticles (Bordereau and Andersen, 1978;Roter et al, 1985;Cox and Willis, 1985;Wolfgang and Riddiford, 1986).…”

mentioning

confidence: 99%

cDNA cloning and deduced amino acid sequence of a major, glycine‐rich cuticular protein from the coleopteran Tenebrio molitor

Charles

Bouhin

Quennedey

et al. 1992

European Journal of Biochemistry

View full text Add to dashboard Cite

In Coleoptera, the elytra (forewings), with a very hard and thick cuticle, protect the membranous and delicate hindwings against mechanical stress. We have isolated and characterized a cDNA encoding a major cuticle protein in Tenebrio molitor, named ACP‐20. The deduced amino acid sequence is roughly tripartite, with two terminal glycine‐rich domains and a central region showing pronounced similarities with some other hard cuticle proteins. Northern blot and in situ hybridization analyses reveal that ACP‐20 gene expression is developmentally regulated since transcript accumulation occurs only in epidermal regions synthesizing hard cuticle and is restricted to the period of preecdysial adult cuticle deposition. Moreover, application of a juvenile hormone analogue prevents the appearance of the transcript, indicating that juvenile hormone, a key molecule involved in the control of insect metamorphosis, negatively regulates the expression of the ACP‐20 gene.

show abstract

The cuticular proteins of Hyalophora cecropia from different anatomical regions and metamorphic stages

Cited by 97 publications

References 20 publications

Hormonal regulation of sequential larval cuticular gene expression

Hormonal regulation of sequential larval cuticular gene expression

Two major cuticular proteins are required for assembly of horizontal laminae and vertical pore canals in rigid cuticle of Tribolium castaneum

cDNA cloning and deduced amino acid sequence of a major, glycine‐rich cuticular protein from the coleopteran Tenebrio molitor

Contact Info

Product

Resources

About