The current taxonomic status of Aspergillus sojae used in shoyu fermentation.

Ushijima, Shigeomi; Hayashi, Kazuya; Murakami, Hisashi

doi:10.1271/bbb1961.46.2365

Cited by 7 publications

(9 citation statements)

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“…Propagation was done on agar plates according to the specifications given in Heerd et al ( 2012 ). It has to be noted that A. sojae ATCC 20235, which is still deposited as A. sojae at the American Type of Culture Collection (ATCC), did not meet the requirements to be classified as A. sojae on the basis of morphological parameters (Ushijima et al 1982 ), and has been reclassified as A. oryzae based on the alpA restriction fragment length polymorphism (RFLP) (Heerikhuisen et al 2005 ).…”

Section: Methodsmentioning

confidence: 99%

Efficient polygalacturonase production from agricultural and agro-industrial residues by solid-state culture of Aspergillus sojae under optimized conditions

2014

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Previously identified fungal pectinase producers of the species Aspergillus sojae were used for optimization of polygalacturonase production in solid-state fermentation applying Design of Experiment. The effects of media composition and several process parameters, like inoculum size, moisture level, incubation time and temperature on polygalacturonase activity were studied in screening and optimization investigations. Utilization of agricultural and agro-industrial by-products provided the establishment of a cost-efficient and sustainable process for enzyme production. Comparison of pectinase production by A. sojae ATCC 20235 and A. sojae CBS 100928 under optimized conditions yielded 6.9 times higher polygalacturonase activity by A. sojae ATCC 20235. Highest enzyme yield (909.5 ± 2.7 U/g) was obtained by A. sojae ATCC 20235 after 8 days at 30°C applying 30% sugar beet pulp as inducer substrate in combination with wheat bran as medium wetted at 160% with 0.2 M HCl. Furthermore, an overview of pectinolytic enzyme activities present in the extracts of both strains is provided. Protein profiles of both strains are given by SDS-PAGE electrophoresis, as well as zymograms for pectinolytic enzymes in comparison to commercial pectinase preparations.Electronic supplementary materialThe online version of this article (doi:10.1186/2193-1801-3-742) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Efficient polygalacturonase production from agricultural and agro-industrial residues by solid-state culture of Aspergillus sojae under optimized conditions

2014

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“…The A. sojae ATCC 20235 strain was purchased from Procochem Inc. (Teddington, United Kingdom), an authorized distributor of the ATCC (American Type of Culture Collection) in Europe. It has to be remarked that A. sojae ATCC 20235 does not meet the requirements to be classified as A. sojae on the basis of morphological parameters, and thus it has been reclassified as Aspergillus oryzae . The mutant strains M3 and DH 56 were generated by Heerd et al The A. sojae mutant Guserbiot 2.230 was developed by Guserbiot SLU (Vitoria‐Gasteiz, Spain).…”

Section: Methodsmentioning

confidence: 99%

A novel pectin‐degrading enzyme complex from Aspergillus sojaeATCC 20235 mutants

et al. 2014

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“…It has to be noted that A. sojae ATCC 20235, which is still deposited as A. sojae at the ATCC, did not meet the requirements to be classified as A. sojae on the basis of morphological parameters (Ushijima et al 1982) and has been reclassified as Aspergillus oryzae based on the alpA restriction fragment length polymorphism (RFLP) (Heerikhuisen et al 2005).…”

Section: Microorganismsmentioning

confidence: 99%

Microbial strain improvement for enhanced polygalacturonase production by Aspergillus sojae

Heerd

Tarı

Fernández‐Lahore

2014

Appl Microbiol Biotechnol

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Strain improvement is a powerful tool in commercial development of microbial fermentation processes. Strains of Aspergillus sojae which were previously identified as polygalacturonase producers were subjected to the costeffective mutagenesis and selection method, the so-called random screening. Physical (ultraviolet irradiation at 254 nm) and chemical mutagens (N-methyl-N′-nitro-Nnitrosoguanidine) were used in the development and implementation of a classical mutation and selection strategy for the improved production of pectic acid-degrading enzymes. Three mutation cycles of both mutagenic treatments and also the combination of them were performed to generate mutants descending from A. sojae ATCC 20235 and mutants of A. sojae CBS 100928. Pectinolytic enzyme production of the mutants was compared to their wild types in submerged and solid-state fermentation. Comparing both strains, higher pectinase activity was obtained by A. sojae ATCC 20235 and mutants thereof. The highest polygalacturonase activity (1,087.2±151.9 U/g) in solid-state culture was obtained by mutant M3, which was 1.7 times increased in comparison to the wild strain, A. sojae ATCC 20235. Additional, further mutation of mutant M3 for two more cycles of treatment by UV irradiation generated mutant DH56 with the highest polygalacturonase activity (98.8±8.7 U/mL) in submerged culture. This corresponded to 2.4-fold enhanced polygalacturonase production in comparison to the wild strain. The results of this study indicated the development of a classical mutation and selection strategy as a promising tool to improve pectinolytic enzyme production by both fungal strains.

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The current taxonomic status of Aspergillus sojae used in shoyu fermentation.

Abstract: In 1944, Sakaguchi and Yamada isolated some characteristic yellow green Aspergillus strains, which produced prominently echinulate conidia and smooth-walled co

Cited by 7 publications

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Efficient polygalacturonase production from agricultural and agro-industrial residues by solid-state culture of Aspergillus sojae under optimized conditions

Efficient polygalacturonase production from agricultural and agro-industrial residues by solid-state culture of Aspergillus sojae under optimized conditions

A novel pectin‐degrading enzyme complex from Aspergillus sojaeATCC 20235 mutants

Microbial strain improvement for enhanced polygalacturonase production by Aspergillus sojae

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