The current state of play of rodent models to study the role of vitamin D in UV-induced immunomodulation

Gorman, Shelley; Hart, Prudence

doi:10.1039/c2pp25108f

Cited by 8 publications

(6 citation statements)

References 79 publications

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“…The serum concentration of activated vitamin D, 1,25(OH) 2 D 3 , was not significantly different between the D++ and D+ groups. As previously observed 29 , 1,25(OH) 2 D concentrations in the D− mice were lower than in the other groups, though this did not reach statistical significance (p = 0.087). There was no difference in serum calcium levels between the three groups.…”

Section: Resultssupporting

confidence: 66%

High Dose Vitamin D supplementation alters faecal microbiome and predisposes mice to more severe colitis

Ghaly

Kaakoush

Lloyd

et al. 2018

Sci Rep

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Vitamin D has been suggested as a possible adjunctive treatment to ameliorate disease severity in human inflammatory bowel disease. In this study, the effects of diets containing high (D++, 10,000 IU/kg), moderate (D+, 2,280 IU/kg) or no vitamin D (D−) on the severity of dextran sodium sulphate (DSS) colitis in female C57Bl/6 mice were investigated. The group on high dose vitamin D (D++) developed the most severe colitis as measured by blinded endoscopic (p < 0.001) and histologic (p < 0.05) assessment, weight loss (p < 0.001), drop in serum albumin (p = 0.05) and increased expression of colonic TNF-α (p < 0.05). Microbiota analysis of faecal DNA showed that the microbial composition of D++ control mice was more similar to that of DSS mice. Serum 25(OH)D3 levels reduced by 63% in the D++ group and 23% in the D+ group after 6 days of DSS treatment. Thus, high dose vitamin D supplementation is associated with a shift to a more inflammatory faecal microbiome and increased susceptibility to colitis, with a fall in circulating vitamin D occurring as a secondary event in response to the inflammatory process.

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Section: Resultssupporting

confidence: 66%

High Dose Vitamin D supplementation alters faecal microbiome and predisposes mice to more severe colitis

Ghaly

Kaakoush

Lloyd

et al. 2018

Sci Rep

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“…Vitamin D induces tolerance through effects on dendritic cells (DCs) and regulatory T cells [1], [45]. In addition to the effects of vitamin D deficiency on BALF granulocytes in male mice, we also observed significantly (p<0.05) increased numbers of ADLN cells in male (vitD+ 7.3±0.6×10 6 ; vitD− 1.0±0.1×10 7 cells/mouse, mean±SEM) but not female (vitD+ 8.0±1.7×10 6 ; vitD− 9.3±1.8×10 6 cells/mouse, mean±SEM) vitamin D-deficient mice, 24 h post-OVA challenge (n = 8–9 mice/treatment pooled from 3 independent experiments).…”

Section: Resultsmentioning

confidence: 99%

Reversible Control by Vitamin D of Granulocytes and Bacteria in the Lungs of Mice: An Ovalbumin-Induced Model of Allergic Airway Disease

et al. 2013

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Vitamin D may be essential for restricting the development and severity of allergic diseases and asthma, but a direct causal link between vitamin D deficiency and asthma has yet to be established. We have developed a ‘low dose’ model of allergic airway disease induced by intraperitoneal injection with ovalbumin (1 µg) and aluminium hydroxide (0.2 mg) in which characteristics of atopic asthma are recapitulated, including airway hyperresponsiveness, antigen-specific immunoglobulin type-E and lung inflammation. We assessed the effects of vitamin D deficiency throughout life (from conception until adulthood) on the severity of ovalbumin-induced allergic airway disease in vitamin D-replete and -deficient BALB/c mice using this model. Vitamin D had protective effects such that deficiency significantly enhanced eosinophil and neutrophil numbers in the bronchoalveolar lavage fluid of male but not female mice. Vitamin D also suppressed the proliferation and T helper cell type-2 cytokine-secreting capacity of airway-draining lymph node cells from both male and female mice. Supplementation of initially vitamin D-deficient mice with vitamin D for four weeks returned serum 25-hydroxyvitamin D to levels observed in initially vitamin D-replete mice, and also suppressed eosinophil and neutrophil numbers in the bronchoalveolar lavage fluid of male mice. Using generic 16 S rRNA primers, increased bacterial levels were detected in the lungs of initially vitamin D-deficient male mice, which were also reduced by vitamin D supplementation. These results indicate that vitamin D controls granulocyte levels in the bronchoalveolar lavage fluid in an allergen-sensitive manner, and may contribute towards the severity of asthma in a gender-specific fashion through regulation of respiratory bacteria.

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“…Cutaneous UVR exposure causes systemic immune suppression in humans 1 but is also a major source of vitamin D. Reduced vitamin D levels may increase MS risk. 2 , 3 However, several studies 3 – 6 suggest that immunoregulation by UVR is by both vitamin D-dependent and vitamin D-independent pathways. Vitamin D and sun exposure are additive, independent risk factors for MS development.…”

Section: Introductionmentioning

confidence: 99%

A randomised, controlled clinical trial of narrowband UVB phototherapy for clinically isolated syndrome: The PhoCIS study

Hart

Jones

Trend

et al. 2018

Multiple Sclerosis Journal - Experimental, Translational and Cl

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BackgroundThe natural history of multiple sclerosis (MS) typically presents with the clinically isolated syndrome (CIS), an episode of neurological symptoms caused by central nervous system inflammation or demyelination that does not fulfil the diagnostic criteria for MS.ObjectiveAs preclinical studies have suggested that exposure to ultraviolet radiation (UVR) could regulate the development of MS, the Phototherapy for CIS (PhoCIS trial) was established to examine the effects of narrowband UVB phototherapy on patients with CIS, and their conversion to MS.MethodsOf the 20 participants, half received 24 sessions of narrowband UVB exposure over eight weeks; participants in both arms were followed for 12 months. All participants were supplemented to 25-hydroxyvitamin D3 levels of >80 nmol/l.ResultsBy 12 months, 100% of those in the no phototherapy arm and 70% in the phototherapy arm had converted to MS, although this difference was not statistically significant.ConclusionThis study provides a basis for further studies to determine if there are any benefits of the therapeutic effects of narrowband UVB radiation on MS progression.

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The current state of play of rodent models to study the role of vitamin D in UV-induced immunomodulation

Cited by 8 publications

References 79 publications

High Dose Vitamin D supplementation alters faecal microbiome and predisposes mice to more severe colitis

High Dose Vitamin D supplementation alters faecal microbiome and predisposes mice to more severe colitis

Reversible Control by Vitamin D of Granulocytes and Bacteria in the Lungs of Mice: An Ovalbumin-Induced Model of Allergic Airway Disease

A randomised, controlled clinical trial of narrowband UVB phototherapy for clinically isolated syndrome: The PhoCIS study

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