The Current Diversity and Distribution of the Simple Thalloid Genus Apopellia (Marchantiophyta): Evidence from an Integrative Taxonomic Study

Abstract

An integrative study of expanded sampling of Apopellia species, including the topotype of Apopellia megaspora, made it possible to clarify the taxonomic position and distribution of the species of the genus. The ITS1-2 and trnL-F sequence data were obtained for 36 molecularly tested specimens, including the topotype Apopellia megaspora, that together with data previously deposited in GenBank, support the treatment of Apopellia as a separate genus and A. alpicola as a distinct species, as well as radically change the idea on the distribution of the species of the genus. It is shown that A. megaspora is an American-Asian species with single records in Europe, whereas A. alpicola is a West-American-Eurasian species widespread in western North America and occurring scattered in Eurasia. Both species occur in the mountains of western North America and south Siberia. A. endiviifolia is widespread in Europe, scattered in Asia and so far not confirmed for America. The expanded sampling of Apopellia spp. allows us to clarify the morphological features of the species of the genus, and microphotographs illustrate the more-important morphological features.

Bryophyte molecular barcoding records. 9

“…Sequencing of the specimen confirmed our assumptions and allowed us to attribute specimen to A. megaspora. The nucleotide sequence diversity of tested specimen by ITS2 consists 0-0.6% with multiply sampled accessions from different region of Russia and till 2% with accession from U.S.A., which slightly exceeds previously published level of infraspecific variability -0.5-1.6% (Konstantinova et al, 2023). It is the first record of the species to Murmansk Province and the third record for Europe.…”

“…It is the first record of the species to Murmansk Province and the third record for Europe. Previously A. megaspora was recorded for single locality in Arkhangelsk Province and Republic of Komi (Konstantinova et al, 2023). ACKNOWLEDGMENTS The work of E.A.…”

“…Apopellia megaspora was described from Eastern North America (Schuster, 1981) and has long been considered as endemic to Eastern North America. However, recently, based on an integrative approach, the species has also been found in Western North America and in Russia (Schütz et al, 2016;Konstantinova et al, 2023). Identification of specimens of the genus Apopellia without sporophyte and perianthia presents considerable difficulties and leads to numerous errors.…”