“…10AS cells show a strong propensity to metastasize via the lymphatics in vivo (Nestl et al, 2001), in contrast to C6 glioma cells, which produce leptomenigeal metastases from intracisternal sites (Herrlinger et al, 1996). Grafting of tumors and tumor cell lines onto the CAM has previously been performed to study the interaction of the tumors with the chorionic epithelium and the blood vessels of the CAM (Armstrong et al, 1982;Brooks et al, 1994;Campbell 1949;Dagg et al, 1956;Kaufman et al, 1956;Mostafa et al, 1980;Murphy 1913;Ossowski and Reich 1980;Papoutsi et al, 2000b;Scher et al, 1976;Schrek and Avery 1937;Stevenson 1918). The fact that the CAM also contains a regular pattern of lymphatics accompanying the blood vessels (Budge, 1887;Oh et al, 1997;Papoutsi et al, 2000a;Wilting et al, 1996) opens the additional possibility of studying interactions of tumors with the lymphatics.…”