“…Nasal RPMI2650 cells (Sigma Aldrich, 88031602, Vienna, Austria) originating from squamous cell carcinoma of nasal epithelium were cultured in Eagle's Modified Essential Medium (EMEM, Sigma Aldrich, M0325) supplemented with 10% ELAREM™ FD (PL Bioscience, Aachen, Germany) and 1% Antibiotic Antimycotic solution (Sigma Aldrich, A5955, Vienna, Austria). The nasal epithelial cells were seeded at an optimized seeding density of 1 × 10 6 cells/cm 2 , as previously described [42]. For experiments outside of a CO 2 incubator, medium was supplemented with 100 mM HEPES buffer solution (1% v/v in complete culture medium).…”