The cultivable endophytic fungal community of <i>Scutellaria baicalensis</i>: diversity and relevance to flavonoid production by the host

Cui, Xiao-Xuan; Wang, Lei; Fang, Hao; Zheng, Yuguang; Su, Chun-Yan

doi:10.1080/15592324.2022.2068834

Cited by 5 publications

(3 citation statements)

References 34 publications

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“…Generally, temperature can affect spore development (zoospore release). Low temperatures can increase humidity, causing the stem bark of sungkai to become more humid [47][48][49][50]. Stem bark that contains water can be a suitable habitat for spores (zoospores), which is characteristic of Pythium, to breed.…”

Section: Discussionmentioning

confidence: 99%

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Secondary metabolites of endophytic fungii isolated from the stem bark of Sungkai (Peronema canescens Jack.)

OKTIANSYAH,

ELFITA,

WIDJAJANTI

et al. 2024

jrp

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Infectious diseases are a global challenge today. One preventive measure is to maintain and improve humans' immunity. Many people consume sungkai, a medicinal plant that is believed to cut down the exposure of severe covid-19 because it is related to antioxidant and antibacterial activity. This research investigated the antioxidant and antimicrobe agents of fungal endophyte extracts from the stem bark of sungkai. Endophytic fungal was isolated from the fresh tissue of the stem bark of sungkai and identified morphologically. The antioxidant of the endophytic fungi extract was tested with the DPPH, and antibacterial agent was tested by using the paper disc diffusion method. Molecular identification of endophytic fungi was done to extract that showed the most potential antioxidant and antibacterial activity. The pure compounds were isolated by chromatographic techniques. Structural determinations of the compounds were accomplished using a spectroscopic method, comprising 1D and 2D NMR. Twenty isolates of fungal endophyte were found residing in the stem bark of sungkai, specifically RB1-RB20. Isolates RB4 and RB6 showed the most potential antioxidant and antibacterial activity and were identified molecularly. The results of molecular identification showed that RB4 and RB6 were Curvularia intermedia and Colletotrichum cliviicola. Based on the spectroscopic analysis, the compounds identified from the two fungi were the different compound, specifically 3hydroxy-4(hydroxy(4-hydroxyphenyl)methyl)-ɣ-butyrolactone (1) and 5-hydroxy-4-(hydroxymethyl)-2H-pyran-2-on (2). Compound 1 and 2 showed antibacterial and antioxidant in strong to moderate. These compounds and the endophytic fungi extract can be used as a new ingredient for medicine because it has antioxidant and antibacterial activity with further research.

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Section: Discussionmentioning

confidence: 99%

“…The Antioxidant was tested by using DPPH with different concentrations [50]. The absorbances were measured by using spectrophotometer UVVis at 517 nm, and ascorbic acid as positive control.…”

Section: Antioxidant Activity Testmentioning

confidence: 99%

Secondary metabolites of endophytic fungii isolated from the stem bark of Sungkai (Peronema canescens Jack.)

OKTIANSYAH,

ELFITA,

WIDJAJANTI

et al. 2024

jrp

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show abstract

“…The relative abundance of the fungal community was expressed by community barplots both on the phylum and genus levels. The correlations between the fungal communities (relative abundance before 50%) and flavonoid contents (or antioxidant abilities) were conducted by Spearman analysis and expressed by the Spearman correlation heatmap ( Cui et al, 2022 ).…”

Section: Methodsmentioning

confidence: 99%

Endophytic fungi in buckwheat seeds: exploring links with flavonoid accumulation

Zhong,

Niu,

Xiang

et al. 2024

Front. Microbiol.

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Buckwheat is a famous edible and medicinal coarse cereal which contain abundant of bioactive flavonoids, such as rutin. In this study, the composition and diversity of endophytic fungi in eight different buckwheat seeds were analyzed by high-throughput sequencing of ITS rDNA. Results showed that, the fungal sequences reads were allocated to 272 OTUs, of them, 49 OTUs were shared in eight buckwheat seeds. These endophytic fungi could be classified into 6 phyla, 19 classes, 41 orders, 79 families, 119 genera, and 191 species. At genus level, Alternaria sp. was the domain fungal endophyte. Besides, fungal endophytes belonged to the genera of Epicocum, Cladosporium, Botrytis, Filbobasidium, Stemphylium, and Vishniacozyma were highly abundant in buckwheat seeds. The total flavonoids and rutin contents in tartary buckwheat cultivars (CQ, XQ, CH, K2) were much higher than those in common buckwheat cultivars (HT, T2, T4, T8). For tartary buckwheat cultivars, the total flavonoids and rutin contents were ranging from 2.6% to 3.3% and 0.9% to 1.3%, respectively. Accordingly, the tartary buckwheat samples displayed stronger antioxidant activity than the common buckwheat. Spearman correlation heat map analysis was successfully found that certain fungal species from the genera of Alternaria, Botryosphaeria, Colletorichum and Diymella exhibited significant positive correlation with flavonoids contents. Results of this study preliminary revealed the fungi-plant interaction relationship at secondary metabolite level, and could provide novel strategy for increasing the flavonoids accumulation of buckwheat seeds, as well as improving their quality.

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Foliar phenols and flavonoids level in pteridophytes: an insight to culturable fungal endophyte colonisation

Singha,

Sharma,

Saha

et al. 2024

Arch Microbiol

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The cultivable endophytic fungal community of Scutellaria baicalensis: diversity and relevance to flavonoid production by the host

Cited by 5 publications

References 34 publications

Secondary metabolites of endophytic fungii isolated from the stem bark of Sungkai (Peronema canescens Jack.)

Secondary metabolites of endophytic fungii isolated from the stem bark of Sungkai (Peronema canescens Jack.)

Endophytic fungi in buckwheat seeds: exploring links with flavonoid accumulation

Foliar phenols and flavonoids level in pteridophytes: an insight to culturable fungal endophyte colonisation

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