The cucurbit downy mildew pathogen <i>Pseudoperonospora cubensis</i>

Savory, Elizabeth A.; Granke, L. L.; Quesada‐Ocampo, Lina M.; Varbanova, Marina; Hausbeck, M. K.; Day, Brad

doi:10.1111/j.1364-3703.2010.00670.x

Cited by 159 publications

(108 citation statements)

References 48 publications

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“…Synthetic fungicides, such as members of the azole family, though of widespread use in controlling food and plant pathogens and postharvest diseases, are being used in a limited way due to the emergence of fungal resistance over long-term use (Makovitzki et al, 2007;Savory et al, 2011). For example, most zygomycota species, including Mucor species, are resistant to most typical antifungal agents (Gomez-Lopez et al, 2003;Khan et al, 2009), among which M. circinelloides has even shown resistance to broad-spectrum posaconazole and itraconazole (Gomez-Lopez et al, 2003;Khan et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Antifungal activity of rhamnolipids against dimorphic fungi

Sha

Meng

2016

J. Gen. Appl. Microbiol.

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In this paper, rhamnolipids are investigated, for the first time, for their feasibility for inhibiting dimorphic fungi. Rhamnolipids were found to effectively inhibit a dimorphic fungus isolated from tomato plants which was identified as Mucor circinelloides according to characterizations by morphologies as well as 28S rDNA sequences. Rhamnolipids markedly reduced growth of this fungus in both the yeast-like form and the filamentous form. Such an inhibitive effect was similarly obtained with Verticillium dahliae, a representative member of dimorphic fungi, confirming the effectiveness of rhamnolipids in the two growth forms of dimorphic fungi. Interestingly, rhamnolipids showed a greater inhibitive function in the case of the pathogenic growth mode of dimorphic fungi, such as the mycelium growth for M. circinelloides and the yeast-like growth for V. dahliae, than their non-pathogenic modes. The use of rhamnolipids might greatly reduce the frequently-reported drugresistance to the common anti-fungal agents by deterring the possible switch between the two modes of dimorphic fungi. Overall, rhamnolipids as environment-friendly biocontrol agents have a potential use in protecting plants from dimorphic fungi infections, and could also offer guidance toward future research into controlling dimorphic disease infection in humans.

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Section: Introductionmentioning

confidence: 99%

Antifungal activity of rhamnolipids against dimorphic fungi

Sha

Meng

2016

J. Gen. Appl. Microbiol.

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“…In the United States, the threat of Ps. cubensis to cucumber production was successfully mitigated by host resistance until 2004; however, this pathogen has since overcome host resistance and is now a severe threat to growers, especially as it also evolves fungicide resistance (Holmes et al 2006;Savory et al 2011).…”

Section: Host Range and Economic Value Of Ps Cubensismentioning

confidence: 99%

“…However, it was not identified on live plants until 1903 in Moscow by Rostovzev (Lebeda and Cohen 2011). Although the taxonomy of this pathogen has varied, it is currently classified in Kingdom Straminipila, Phylum Oomycota, Class Oomycetes, Order Peronsporales, Family Peronosporaceae, Genus Pseudoperonospora, Species Pseudoperonospora cubensis (Göker et al 2007;Savory et al 2011). Like other oomycetes, Ps.…”

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confidence: 99%

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A genomics perspective on cucurbit-oomycete interactions

Burkhardt

Day

2013

Plant Biotechnology

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Pseudoperonospora cubensis and Phytophthora capsici are plant pathogenic oomycetes that are severe threats to cucurbit cultivation because of the their global distribution, their broad host range among the Cucurbitaceae family, and their ability to overcome susceptibilities to host, environment, and chemical management. Historically, these pathogens have been extensively studied in terms of their life cycles and infection strategies in order to determine appropriate methods to manage disease. In recent years, the genomes of both pathogens have been sequenced, which will lead to greater opportunities for pathogen detection and will help researchers to better understand the host-pathogen interaction. In Ps. cubensis, the transcriptomes of both Ps. cubensis and Cucumis sativus (cucumber) have been sequenced, and this data is being analyzed to determine the function of Ps. cubensis effectors and the role of alternative splicing in the regulation of pathogen gene expression. Previous and ongoing work is being done to determine cucumber genes involved in resistance. In P. capsici, effectors have been identified in the genome sequence, and the genome being used to identify variation in different P. capsici isolates. Future work is needed to give biological meaning to genomics data and to determine mechanisms of pathogenicity in oomycetes and resistance in cucurbits. Herein, we will present an overview of the current and future objectives of genome-based research in this area, describing the molecular mechanisms of pathogen virulence and host response to infection.

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“…Next-generation (genome/ transcriptome) sequencing, resistance/ effector gene characterization (Haas et al 2009, Huang et al 2009, Mueller et al 2009, Raffaele et al 2010, Tian et al 2011, Woycicki et al 2011, micro-examination of the interactions between pathogenic fungi and host plants (Hood & Shew 1996, Hardham & Shan 2009, Savory et al 2010, and systems biology studies (Vleeshouwers et al 2011, Woycicki et al 2011, Nowicki et al 2012b, Raffaele et al 2010, Shibata et al 2010) have all proven highly effective tools in identifying the molecular and cellular basis of pathogen resistance and susceptibility. In particular, microscopic studies provide an exciting body of evidence regarding the pathogen biology or host plant reaction upon the perceived attack (Hood & Shew 1996, Hardham & Shan 2009, Savory et al 2010, Chen & Halterman 2011. These studies, though invaluable as a pathogen diagnostic tool or an indicator of the resistance level exhibited by the host plants, hitherto mostly failed to investigate both the pathogenic fungi and their host plants' responses within one assay.…”

Section: Introductionmentioning

confidence: 99%

A Simple Dual Stain for Detailed Investigations of Plant-Fungal Pathogen Interactions

Nowicki

Lichocka

Nowakowska

et al. 2012

Journal of Fruit and Ornamental Plant Research

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SummaryDramatic increase in confocal microscopy observation output has been gained by optimization of a simple trypan blue and aniline blue dual-stain and its application to two model pathosystems: Pseudoperonospora cubensiscucumber and Phytophthora infestans-tomato. Comparison of two dual-stain methods for confocal microscopy studies of P. cubensis-challenged cucumber leaves indicated the 'mild' approach most successful. This methodology provides simultaneous detection of different pathogen structures layered with the plant defense reactions. Moreover, ImageJ-assisted quantification of plant defense responses renders this method useful for addressing the host plant resistance reactions, as well as investigating the given isolate's pathogenicity. Application of this method for the P. infestans-challenged tomato leaf samples resulted in detection of several fungal infection structures, along with plant defense responses. The dual-stain also enabled detection of a peculiar aniline blue-sensitive material in the pathogen cell walls at the area of its hyphae emerging through the leaf stomata. Results presented herein indicate this method is applicable for detailed (possibly quantitative) investigations of multiple plant-fungal pathosystems.

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The cucurbit downy mildew pathogen Pseudoperonospora cubensis

Cited by 159 publications

References 48 publications

Antifungal activity of rhamnolipids against dimorphic fungi

Antifungal activity of rhamnolipids against dimorphic fungi

A genomics perspective on cucurbit-oomycete interactions

A Simple Dual Stain for Detailed Investigations of Plant-Fungal Pathogen Interactions

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