The cSNP scanning and expression analysis of the duck FTH1 gene

Abstract

Materials and methods Ethics statementAll procedures involving the care and use of animals conformed to US National Institutes of Health guidelines Abstract: Ferritin, heavy polypeptide 1 (FTH1) is a kind of ferritin complex that catalyzes the conversion of Fe(II) into Fe(III) to protect the cell from oxidative damage. Recent research has shown that FTH1 is also associated with several disease-related processes, such as inflammation and tumor progression and so on. In this study, the coding sequence (CDS) of FTH1 in Jinding ducks was cloned. The CDS sequence revealed an open reading frame of 546 nucleotides encoding a protein with 181 amino acids. Based on this sequence, the duck FTH1 protein is predicted to have conserved domains typical of eukaryotic ferritin. Only one synonymous mutation (c.447T > C) of the coding sequence of the duck FTH1 gene was identified in ducks. Quantitative RT-PCR results revealed that FTH1 expression in the liver and spleen was significantly downregulated after duck hepatitis virus 1 infection. Meanwhile, in DF-1 cells transfected with an FTH1 plasmid, expression of the antiviral marker gene Mx1 was significantly upregulated. These results suggest that the coding sequence of the duck FTH1 gene was highly conserved and FTH1 plays an important role in antivirus activity.

Screening of immune-related differentially expressed genes from primary lymphatic organs of broilers fed with probiotic bacillus cereus PAS38 based on suppression subtractive hybridization

“…FTH1 (Ferritin heavy chain 1) is the main subunit of ferritin, responsible for the oxidation and integration of ferrous ions to maintain the balance of ferrous ions in the body [ 46 ]. Some studies have shown that FTH1 is related to the egg-laying traits of chickens [ 47 ], and some studies have also proven that expression of FTH1 changed significantly after poultry were infected with some pathogenic bacteria and viruses [ 23 , 48 ]. After the interference of FTH1 , the five cytokines: IL-1B , IL-2 , IL-6 , TLR4 and MYD88 were all significantly down-regulated.…”

Screening of differentially expressed immune-related genes from spleen of broilers fed with probiotic Bacillus cereus PAS38 based on suppression subtractive hybridization

“…These distinct results suggest that chickens may have different defense mechanisms against bacterial and viral infections. Zhang et al [28] found that the expression of FTH1 in liver and spleen of Jingding duck was significantly down-regulated after infection with duck hepatitis virus 1, and the expression of antiviral marker gene MX1 was significantly up-regulated in DF-1 cells transfected with the FTH1 plasmid. Sun et al [29] showed that after FTH1 silencing, the intracellular viability of Brucella decreased, and the apoptotic rate of macrophages increased.…”

Circular RNAs in ferroptosis: regulation mechanism and potential clinical application in disease

“…Zheng et al (2021b) proposed that circSnx12 is involved in ferroptosis during HF by targeting the miR-224-5p/ferritin heavy chain 1 (FTH1) axis. FTH1 is a ferritin complex that catalyzes the conversion of Fe 2+ into Fe 3+ to protect the cell from oxidative damage (Zhang et al, 2017). CircSnx12 acts as a sponge for miR-224-5p, and FTH1 is a target gene of miR-224-5p.…”

“…HF is a complex syndrome with a high mortality rate (Zhang et al, 2017). The prognosis of patients with HF is generally poor (Zhang et al, 2017).…”

“…HF is a complex syndrome with a high mortality rate (Zhang et al, 2017). The prognosis of patients with HF is generally poor (Zhang et al, 2017). Therefore, it is necessary to identify and develop appropriate treatment strategies to improve the prognosis and quality of life of HF patients (Zhang et al, 2017).…”