The crystal structure and identification of NQM1/YGR043C, a transaldolase from <i>Saccharomyces cerevisiae</i>

Huang, Hua; Hui, Rutai; Xu, Li; Shu, Tong; Zhu, Zhiqiang; Niu, Liwen; Teng, Maikun

doi:10.1002/prot.22237

Cited by 24 publications

(18 citation statements)

References 21 publications

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“…2). These catalytic sites are believed to be responsible for Schiff-base formation and aldose cleavage (15,16). The single amino acid difference was that a serine, an amino acid residue responsible for substrate-binding in ascomycetous yeast, was substituted with a glycine in M. megachiliensis (Fig.…”

Section: Discussionmentioning

confidence: 97%

“…These results suggest the importance of the transaldolase isoform for oxidoereduction balance through the glycolytic and pentose phosphate pathways in living cells (12,19). However, the function of the other isoform, NQM1, remains unclear (16).…”

mentioning

confidence: 95%

“…As a key enzyme in the PPP, TAL catalyzes the reversible transfer of the dihydroxyacetone moiety between sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate, and also between erythrose-4-phosphate and fructose-6-phosphate (16,17). Erythrose-4-phosphate condenses with phosphoenolpyruvate (PEP) derived from the glycolytic pathway to form chorismate, the substrate of the shikimic pathway that leads to the production of aromatic amino acids and many other aromatic compounds (18).…”

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confidence: 99%

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Two transaldolase isogenes from Moniliella megachiliensis behave in a different way depending on the stress class

Iwata

Mizushima

Kobayashi

et al. 2015

Journal of Bioscience and Bioengineering

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Section: Discussionmentioning

confidence: 97%

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confidence: 95%

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confidence: 99%

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Two transaldolase isogenes from Moniliella megachiliensis behave in a different way depending on the stress class

Iwata

Mizushima

Kobayashi

et al. 2015

Journal of Bioscience and Bioengineering

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“…TAL1 encodes a transaldolase which catalyses a reaction in the non-oxidative phase of the PPP, and in concordance with the screen results, deletion of this gene does not alter sensitivity to TMPyP4. This suggests that either the reaction Tal1 catalyses can be sufficiently carried out by a functional homologue (such as Nqm1 [29]) or that TAL1 deletion does not result in metabolic changes that cause TMPyP4-sensitivity.…”

Section: Resultsmentioning

confidence: 99%

Pentose Phosphate Pathway Function Affects Tolerance to the G-Quadruplex Binder TMPyP4

et al. 2013

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G-quadruplexes form in guanine-rich regions of DNA and the presence of these structures at telomeres prevents the activity of telomerase in vitro. Ligands such as the cationic porphyrin TMPyP4 stabilise G-quadruplexes and are therefore under investigation for their potential use as anti-cancer drugs. In order to investigate the mechanism of action of TMPyP4 in vivo, we carried out a genome-wide screen in the budding yeast Saccharomyces cerevisiae. We found that deletion of key pentose phosphate pathway (PPP) genes increased the sensitivity of yeast to the presence of TMPyP4. The PPP plays an important role in the oxidative stress response and sensitivity to TMPyP4 also increased when genes involved in the oxidative stress response, CCS1 and YAP1, were deleted. For comparison we also report genome wide-screens using hydrogen peroxide, which causes oxidative stress, RHPS4, another G-quadruplex binder and hydroxyurea, an S phase poison. We found that a number of TMPyP4-sensitive strains are also sensitive to hydrogen peroxide in a genome-wide screen. Overall our results suggest that treatment with TMPyP4 results in light-dependent oxidative stress response in budding yeast, and that this, rather than G-quadruplex binding, is the major route to cytotoxicity. Our results have implications for the usefulness and mechanism of action of TMPyP4.

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“…The automatic understanding of a sentence requires from the web agent different types of abilities: recognizing words and associating them with lexical information (morphological analysis); structure the sentence with a grammar (parsing), understand the sentence with semantic rules (semantic analysis) and take into account the context (pragmatic analysis). Huangs [20] has shown that the performances of syntactic similarity based on the Jaccard index and the Dice index are very close and that they are significantly better than those of the Euclidean distance and the Levenshtein distance. The distance from Levenshtein is widely used in linguistics and bioinformatics as well as for the recognition of text blocks.…”

Section: Similarity Measures Between Sentencesmentioning

confidence: 99%

A Chatbot for Automatic Processing of Learner Concerns in an Online Learning Platform

Bakouan¹,

Kamagate²,

Kone³

et al. 2018

ijacsa

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Abstract-In this article, we present a chatbot model that can automatically respond to learners' concerns on an online training platform. The proposed chatbot model is based on an adaptation of the similarity of Dice to understand the concerns of learners. The first phase of this approach allows selecting the preestablished concerns that the teacher has in a knowledge base which are closest to those posed by the learner. The second phase consists of selecting among these k most appropriate concerns based on a measure of similarity built on the concept of domain keywords. The experimentation of the prototype of this chatbot makes it possible to find the adequate answers. In the case, where the question refers to a question from the teacher, the learner is asked if the question identified is the one he was referring to. If he answers in the affirmative, the instructions associated with his request are sent to him. If not, the learner's concern is sent to the human tutor. The hybridization of this chatbot with the human agent comes to enrich the initial knowledge base of the chatbot. The results obtained with the concept based on the keywords of the domain are encouraging. The learner's comprehension rate is above 50% when applying the concept of domain keywords while the measure of Dice is below 50%.

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The crystal structure and identification of NQM1/YGR043C, a transaldolase from Saccharomyces cerevisiae

Cited by 24 publications

References 21 publications

Two transaldolase isogenes from Moniliella megachiliensis behave in a different way depending on the stress class

Two transaldolase isogenes from Moniliella megachiliensis behave in a different way depending on the stress class

Pentose Phosphate Pathway Function Affects Tolerance to the G-Quadruplex Binder TMPyP4

A Chatbot for Automatic Processing of Learner Concerns in an Online Learning Platform

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