The Cryptocercus punctulatus species complex (Dictyoptera: Cryptocercidae) in the eastern United States: Comparison of cuticular hydrocarbons, chromosome number, and DNA sequences

Everaerts, Claude; Maekawa, Kazuyuki; Farine, Jean-Pierre; Shimada, Keisuke; Luykx, Peter; Brossut, Rémy; Nalepa, Christine A.

doi:10.1016/j.ympev.2008.03.011

Cited by 21 publications

(34 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Twenty-three insect species, including representatives from 4 families of lower termites plus Cryptocercus cockroaches, were collected (see Table S1 in the supplemental material). The 3 Cryptocercus samples were from distinct populations of the Cryptocercus punctulatus species complex, many of which have different chromosome numbers (22). The paunch (P3) compartments from hindguts of live insects were dissected, and the contents were collected into Trager's solution U (23) and stored at Ϫ20°C.…”

Section: Methodsmentioning

confidence: 99%

The Role of Host Phylogeny Varies in Shaping Microbial Diversity in the Hindguts of Lower Termites

Tai

James

Nalepa

et al. 2015

Appl Environ Microbiol

View full text Add to dashboard Cite

eThe hindguts of lower termites and Cryptocercus cockroaches are home to a distinct community of archaea, bacteria, and protists (primarily parabasalids and some oxymonads). Within a host species, the composition of these hindgut communities appears relatively stable, but the evolutionary and ecological factors structuring community composition and stability are poorly understood, as are differential impacts of these factors on protists, bacteria, and archaea. We analyzed the microbial composition of parabasalids and bacteria in the hindguts of Cryptocercus punctulatus and 23 species spanning 4 families of lower termites by pyrosequencing variable regions of the small-subunit rRNA gene. Especially for the parabasalids, these data revealed undiscovered taxa and provided a phylogenetic basis for a more accurate understanding of diversity, diversification, and community composition. The composition of the parabasalid communities was found to be strongly structured by the phylogeny of their hosts, indicating the importance of historical effects, although exceptions were also identified. Particularly, spirotrichonymphids and trichonymphids likely were transferred between host lineages. In contrast, host phylogeny was not sufficient to explain the majority of bacterial community composition, but the compositions of the Bacteroidetes, Elusimicrobia, Tenericutes, Spirochaetes, and Synergistes were structured by host phylogeny perhaps due to their symbiotic associations with protists. All together, historical effects probably resulting from vertical inheritance have had a prominent role in structuring the hindgut communities, especially of the parabasalids, but dispersal and environmental acquisition have played a larger role in community composition than previously expected.C ryptocercus cockroaches and their sister lineage, the lower termites (1), are both dependent on diverse communities of microorganisms in their hindguts to gain nutrition from lignocellulose (reviewed in references 2 and 3). These are fascinating and complex communities dominated by parabasalid (Parabasalia) and oxymonad (Preaxostyla) protists (4) as well as numerous bacterial lineages, including Actinobacteria, Bacteroidetes, Elusimicrobia, Firmicutes, Proteobacteria, Spirochaetes,. Moreover, a large proportion of the bacteria form obligate symbiotic interactions with the protists (see, for example, references 8-14), so the community contains multiple levels of symbiosis. Because of the diversity of symbionts, the hindguts of Cryptocercus cockroaches and lower termites can be used as model systems to study the ecology and interactions between protists and bacteria. Here, we investigate the ecological and evolutionary differences between protists and bacteria in structuring community composition. These differences are critical to understanding the diversification and adaptation of microbes, the stability and resilience of community structure, and the maintenance of ecosystem functions, particularly in regard to understanding lignocellulose digestion...

show abstract

Section: Methodsmentioning

confidence: 99%

The Role of Host Phylogeny Varies in Shaping Microbial Diversity in the Hindguts of Lower Termites

Tai

James

Nalepa

et al. 2015

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…Previous molecular phylogenetic studies have shown that divergent lower-level monophyletic clades on mtDNA gene trees include members of only a single chromosomal race, even though the races themselves may not be monophyletic ( Fig. 1b; Kambhampati et al 1996;Burnside et al 1999;Steinmiller et al 2001;Lo et al 2006;Everaerts et al 2008). Thus, phylogenetic approaches can be used to assign an ''unknown'' individual to a chromosomal race.…”

Section: Methodsmentioning

confidence: 99%

“…Briefly, Kambhampati et al (1996) first reported the existence of four chromosomal races within the complex, and Burnside et al (1999) subsequently described them as separate species. However, the basis for their taxonomic subdivision has been questioned, and so it has not been widely adopted (Nalepa et al 2002;Lo et al 2006;Everaerts et al 2008). There are also conflicting reports on the spatial distributions of the four races, which have been described as either strictly allopatric (Burnside et al 1999), parapatric (Nalepa et al 2002;Everaerts et al 2008), or sympatric (Steinmiller et al 2001).…”

Section: Introductionmentioning

confidence: 99%

“…However, the basis for their taxonomic subdivision has been questioned, and so it has not been widely adopted (Nalepa et al 2002;Lo et al 2006;Everaerts et al 2008). There are also conflicting reports on the spatial distributions of the four races, which have been described as either strictly allopatric (Burnside et al 1999), parapatric (Nalepa et al 2002;Everaerts et al 2008), or sympatric (Steinmiller et al 2001). One likely reason for this uncertainty is because the majority of previous studies with dense (i.e., population-level) sampling have based race assignment on just one individual per site (e.g., Steinmiller et al 2001;Aldrich et al 2004).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

True syntopy between chromosomal races of the Cryptocercus punctulatus wood-roach species complex

Garrick

2016

Insect. Soc.

View full text Add to dashboard Cite

In this paper, genetic evidence for local cooccurrence (syntopy) between members of two chromosomal races within the Cryptocercus punctulatus species complex from the Southern Appalachian Mountains is presented. This phenomenon has not previously been detected, despite extensive sampling and numerous molecular studies of this the sub-social wood-roach spanning the past 20 years. The incidence of syntopy seems rare; it was detected in one of 70 rotting logs sampled across eight states in the southern United States. However, the location where it occurs could become a key focus of research aimed at resolving taxonomic uncertainty in this group.

show abstract

“…As in the recent studies using karyotype and mtDNA markers (Everaerts et al 2008), further molecular survey for these geographic isolates may provide clues to better understanding of the evolutionary trend of the genus Cryptocercus related to the molecular sequence evolution.…”

Section: Introductionmentioning

confidence: 94%

Evolutionary trend of the small subunit nuclear ribosomal DNAs (SSU rDNAs) of wood-feedingCryptocercuscockroaches (Blattaria:Cryptocercus)

Kim

Rho

Kim

et al. 2013

Animal Cells and Systems

View full text Add to dashboard Cite

Park (2013) Evolutionary trend of the small subunit nuclear ribosomal DNAs (SSU rDNAs) of wood-feeding Cryptocercus cockroaches (Blattaria: Cryptocercus), Animal Cells and Systems, 17:1, 36-43, DOI: 10.1080/19768354.2012 The objective of this study is to elucidate the evolutionary rate of Cryptocercus SSU rDNAs based on the relative rate test (RRT) and comparative analysis of the primary sequences. In the RRTs, the evolutionary rate differences (dKs) of the Cryptocercus SSU rDNAs were not statistically significant (0.107 BP B0.996) in all cases, although an Asian Cryptocercus appeared to evolve more slowly than a North American Cryptocercus as indicated by the negative signs of dKs. Contrary to the RRT, the comparative analysis of SSU rDNA sequences revealed some interesting aspects in the evolutionary rates of Cryptocercus SSU rDNAs. In comparison with those of closely (Mastotermes darwiniensis and Tenodera aridifolia) or more distantly related taxa (Locusta migratoria), the SSU rDNAs of the genus Cryptocercus showed that insertions are progressing in a variable region (HV2). The SSU rDNA of Calymmaderus punctulatus species complex (Mountain Lake Biological Station, VA), a North American species, was unusually more elongated in the HV2 than those of the reference taxa or the other congeneric species.

show abstract

The Cryptocercus punctulatus species complex (Dictyoptera: Cryptocercidae) in the eastern United States: Comparison of cuticular hydrocarbons, chromosome number, and DNA sequences

Cited by 21 publications

References 22 publications

The Role of Host Phylogeny Varies in Shaping Microbial Diversity in the Hindguts of Lower Termites

The Role of Host Phylogeny Varies in Shaping Microbial Diversity in the Hindguts of Lower Termites

True syntopy between chromosomal races of the Cryptocercus punctulatus wood-roach species complex

Evolutionary trend of the small subunit nuclear ribosomal DNAs (SSU rDNAs) of wood-feedingCryptocercuscockroaches (Blattaria:Cryptocercus)

Contact Info

Product

Resources

About