The cryoprotective effect of vitamins on human spermatozoa quality: a systematic review and meta-analysis

Ebrahimi, Bahareh; Matavos-Aramyan, Hedieh; Keshtgar, Sara

doi:10.1007/s10561-021-09953-5

Cited by 5 publications

(6 citation statements)

References 51 publications

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“…11,68 In an attempt to neutralize the oxidative stress during cryopreservation, researchers tested the effectiveness of supplementing sperm freezing media with antioxidants. 69,70 Recent systematic reviews and meta-analyses highlighted that supplementing sperm freezing medium with antioxidants may have a cryoprotective impact on sperm quality. 69,70 In the present study, the arctic sperm cryopreservation medium contains two vitamins (vitamin C and inositol).…”

Section: Discussionmentioning

confidence: 99%

“…69,70 Recent systematic reviews and meta-analyses highlighted that supplementing sperm freezing medium with antioxidants may have a cryoprotective impact on sperm quality. 69,70 In the present study, the arctic sperm cryopreservation medium contains two vitamins (vitamin C and inositol).…”

Section: Discussionmentioning

confidence: 99%

“…Curiously, a recent systematic review and meta-analysis indicated that the cryoprotective power of vitamin C is still debated, and an optimal vitamin C dose has not yet been found. 70 In parallel, several in vitro studies indicated that supplementing freezing medium with myoinositol had beneficial effects on sperm cryosurvival rates. 35,71 Nevertheless, here we showed that when arctic sperm cryopreservation medium was used without L. plantarum secretions, sperm cryoinjuries were detected in both normozoospermic and non-normozoospermic samples.…”

Section: Discussionmentioning

confidence: 99%

“…The latter could be the reason behind cryopreservation‐induced sperm DNA fragmentation in infertile men 11,68 . In an attempt to neutralize the oxidative stress during cryopreservation, researchers tested the effectiveness of supplementing sperm freezing media with antioxidants 69,70 . Recent systematic reviews and meta‐analyses highlighted that supplementing sperm freezing medium with antioxidants may have a cryoprotective impact on sperm quality 69,70 .…”

Section: Discussionmentioning

confidence: 99%

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Lactobacillus plantarumsecretions may exert a cryoprotective effect on human sperm motility: A prospective in vitro study

Raad

Fakih²,

Bazzi³

et al. 2023

Andrology

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BackgroundSemen cryopreservation is a widely used procedure for fertility preservation, despite some level of cryodamage that may occur in spermatozoa after thawing. However, there is some evidence that lactobacilli, one of the bacteria found in semen, might benefit sperm quality.ObjectivesThis study aims to determine whether the addition of Lactobacillus plantarum secretions to sperm freezing medium has an impact on sperm motility, morphology, and DNA fragmentation.Materials and methodsThis is a prospective auto‐controlled study. It was conducted on 30 raw semen samples from 30 infertile men attending a fertility center for semen analysis. Before freezing, all the samples were analyzed for motility, morphology, and DNA fragmentation percentages. Each sample was then divided equally into three aliquots. Cryopreservation was performed on each aliquot using one of the following three media: without Lactobacillus plantarum secretions (control group) or with 107 or 108 colony‐forming units/mL Lactobacillus plantarum secretions. Sperm motility, morphology, and DNA integrity were evaluated after the cryopreservation media were added and after semen thawing.ResultsThe results of this study indicated that after thawing, no statistically significant decrease in progressive motility and non‐progressive percentages were detected in the sperm freezing medium supplemented with 108 colony‐forming units/mL Lactobacillus plantarum secretions than the fresh raw semen. Moreover, multivariate linear regression model analyses showed that the progressive motility (p = 0.02), non‐progressive motility (p = 0.016), and non‐motile spermatozoa (p = 0.012) percentages were significantly decreased in the freezing medium (without Lactobacillus plantarum secretions) compared to the fresh raw semen.Discussion and conclusionTo the best of our knowledge, this is the first study showing that Lactobacillus plantarum secretions had a cryoprotective effect on sperm motility when added to the sperm freezing medium. Furthermore, Lactobacillus plantarum secretions were found to protect sperm DNA integrity more effectively than the freezing medium without Lactobacillus plantarum secretions in non‐normozoospermia group. Cryopreservation procedures must therefore be optimized to minimize any iatrogenically induced sperm DNA damage, given the correlation between sperm DNA damage and increased mutation loads in progeny.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Lactobacillus plantarumsecretions may exert a cryoprotective effect on human sperm motility: A prospective in vitro study

Raad

Fakih²,

Bazzi³

et al. 2023

Andrology

View full text Add to dashboard Cite

show abstract

“…In a recent meta-analysis, other AOXs were found to improve the motility and DNA integrity of cryopreserved gametes [212]. The latter study indicated that using AOXs in cryopreservation media could increase motility by 4.60% (95% CI 3.05-6.16), viability by 5.71% (95% CI 1.72-9.71), and DNA integrity by 10.20% (95% CI 7.42-12.98) in cryopreserved-thawed sperm.…”

Section: Aox Supplementation Of Cryopreservation Mediamentioning

confidence: 99%

Oxidative Stress and Assisted Reproduction: A Comprehensive Review of Its Pathophysiological Role and Strategies for Optimizing Embryo Culture Environment

et al. 2022

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Oxidative stress (OS) due to an imbalance between reactive oxygen species (ROS) and antioxidants has been established as an important factor that can negatively affect the outcomes of assisted reproductive techniques (ARTs). Excess ROS exert their pathological effects through damage to cellular lipids, organelles, and DNA, alteration of enzymatic function, and apoptosis. ROS can be produced intracellularly, from immature sperm, oocytes, and embryos. Additionally, several external factors may induce high ROS production in the ART setup, including atmospheric oxygen, CO2 incubators, consumables, visible light, temperature, humidity, volatile organic compounds, and culture media additives. Pathological amounts of ROS can also be generated during the cryopreservation-thawing process of gametes or embryos. Generally, these factors can act at any stage during ART, from gamete preparation to embryo development, till the blastocyst stage. In this review, we discuss the in vitro conditions and environmental factors responsible for the induction of OS in an ART setting. In addition, we describe the effects of OS on gametes and embryos. Furthermore, we highlight strategies to ameliorate the impact of OS during the whole human embryo culture period, from gametes to blastocyst stage.

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Effects of phosphatidylcholine and tocopherol during larval cryopreservation of Pacific oysters (Magallana gigas)

et al. 2023

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The cryoprotective effect of vitamins on human spermatozoa quality: a systematic review and meta-analysis

Cited by 5 publications

References 51 publications

Lactobacillus plantarumsecretions may exert a cryoprotective effect on human sperm motility: A prospective in vitro study

Lactobacillus plantarumsecretions may exert a cryoprotective effect on human sperm motility: A prospective in vitro study

Oxidative Stress and Assisted Reproduction: A Comprehensive Review of Its Pathophysiological Role and Strategies for Optimizing Embryo Culture Environment

Effects of phosphatidylcholine and tocopherol during larval cryopreservation of Pacific oysters (Magallana gigas)

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