The crude venom from the sea anemone Stichodactyla helianthus induces haemolysis and slight peroxidative damage in rat and human erythrocytes

Monroy-Estrada, Heidi I; Segura-Puertas, Lourdes; Galván‐Arzate, Sonia; Santamarı́a, Abel; Sánchez‐Rodríguez, Judith

doi:10.1016/j.tiv.2006.10.003

Cited by 19 publications

(10 citation statements)

References 23 publications

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“…The toxins can lyse cells directly or even make cells more susceptible to damage by hydrolyzing membrane lipids, so that, as a general feature, venoms can be divided into two categories: one including toxins with a direct lytic action upon erythrocytes, and the other including toxins leading to lipid peroxidation and then to lysis, as reported by several authors (Monroy-Estrada et al 2007). It is known that oxidative damage to the red blood cell membrane leads to a reduction in membrane fluidity and an increase in membrane permeability (Marino et al 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Nematocysts can discharge in response to a variety of mechanical and chemical stimuli, as well as in response to occasional contact, and they are considered to be the most sophisticated lethal weapons in the animal kingdom (Tardent 1995 Cnidarian venom is made of a complex mixture of biologically active molecules including 5-hydroxytryptamine, histamine, proteins such as proteases and phospholipases, and small peptides. The nematocyst toxins exert many bioactivities such as haemolytic, cytolytic, clastogenic, enzymatic, cardiotoxic, neurotoxic and insecticidial activities (Mariottini et al 2002;Li et al 2005;Yu et al 2005;Monroy-Estrada et al 2007;Kang et al 2009;Birsa et al 2010). In many cases, cytolytic, haemolytic or neurotoxic effects have been shown by several biological assays, amongst which the haemolytic assay is the most suitable and most used, being highly sensitive and simple to carry out (Marino et al 2008).…”

Section: Introductionmentioning

confidence: 99%

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Haemolytic activity and characterization of nematocyst venom fromPelagia noctiluca(Cnidaria: Scyphozoa)

Trapani

Parrino

Parisi

et al. 2013

Italian Journal of Zoology

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We investigated the haemolytic capacity of the crude venom extracted from isolated nematocysts of Pelagia noctiluca (Cnidaria: Scyphozoa), and evidenced the proteic fractions responsible for this activity. The nematocyst venom was used at various concentrations to evaluate the haemolytic activity and the lysosomal membrane stability of red blood cells of two teleostean species treated with the extract. The nematocyst extract was assayed against erythrocytes of the two teleostean species living in different environments, Carassius auratus as a common freshwater species, and Liza aurata as a representative of seawater species. Experiments on the haemolytic activity of P. noctiluca in the presence of lipid components of erythrocyte membranes showed that sphyngomyelin strongly inhibited this activity. The crude venom was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis SDS-PAGE and high performance liquid chromatography (HPLC) to detect the proteic composition, and it was found that the active haemolytic components of this venom are distributed in at least four protein fractions. The results of our experiments indicated that Pelagia noctiluca venom induces haemolysis and lysosomal membrane destabilisation in both species and that Carassius auratus was more susceptible to jellyfish venom than was Liza aurata. No significant differences in glutathione (GSH) levels were observed between control and treatments; consequently the toxins do not cause the oxidative stress but likely recognize specific targets (i.e. sphyngomyelin) in the plasmatic membrane of red blood cells.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Haemolytic activity and characterization of nematocyst venom fromPelagia noctiluca(Cnidaria: Scyphozoa)

Trapani

Parrino

Parisi

et al. 2013

Italian Journal of Zoology

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“…Out of the 66 marine natural products identified, 26 correspond to structures that were not previously reported. These 26 new chemical entities correspond to three aliphatic polyketides (1-3), one glycolipid (7), two diterpenes (15,16), two steroids (24,25), one triterpenoid saponin (40), and 17 conotoxins (49)(50)(51)(52)(53)(54)(55)(56)(57)(58)(59)(60)(61)(62)(63)(64)(65). Figure 15 displays the overall biogenetic distribution of the reported compounds.…”

Section: Discussionmentioning

confidence: 99%

Marine Natural Products from the Yucatan Peninsula

et al. 2020

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Mexico is one of the three areas of the world with the greatest terrestrial and cultural biological diversity. The diversity of Mexican medicinal flora has been studied for a long time and several bioactive compounds have been isolated. The investigation of marine resources, and particularly the potential of Mexican marine resources, has not been intensively investigated, even though the Yucatan Peninsula occupies 17.4% of the total of the Mexican coast, with great biological diversity in its coasts and the ocean. There are very few studies on the chemistry of natural products from marine organisms that were collected along the coasts of the Yucatan Peninsula and most of them are limited to the evaluation of the biological activity of their organic extracts. The investigations carried out on marine species from the Yucatan Peninsula resulted in the identification of a wide structural variety of natural products that include polyketides, terpenoids, nitrogen compounds, and biopolymers with cytotoxic, antibacterial, antifouling, and neurotoxic activities. This review describes the literature of bioprospecting and the exploration of the natural product diversity of marine organisms from the coasts of the Yucatan Peninsula up to mid-2019.

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“…As a general feature, cnidarian toxins may cause damage through two mechanisms of action: they may create pores into the target membrane, provoking both ions and water unbalance, or may induce oxidative stress events, leading to lipoperoxidation [20,21,22]. …”

Section: Introductionmentioning

confidence: 99%

Pelagia noctiluca (Scyphozoa) Crude Venom Injection Elicits Oxidative Stress and Inflammatory Response in Rats

et al. 2014

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Cnidarian toxins represent a rich source of biologically active compounds. Since they may act via oxidative stress events, the aim of the present study was to verify whether crude venom, extracted from the jellyfish Pelagia noctiluca, elicits inflammation and oxidative stress processes, known to be mediated by Reactive Oxygen Species (ROS) production, in rats. In a first set of experiments, the animals were injected with crude venom (at three different doses 6, 30 and 60 µg/kg, suspended in saline solution, i.v.) to test the mortality and possible blood pressure changes. In a second set of experiments, to confirm that Pelagia noctiluca crude venom enhances ROS formation and may contribute to the pathophysiology of inflammation, crude venom-injected animals (30 µg/kg) were also treated with tempol, a powerful antioxidant (100 mg/kg i.p., 30 and 60 min after crude venom). Administration of tempol after crude venom challenge, caused a significant reduction of each parameter related to inflammation. The potential effect of Pelagia noctiluca crude venom in the systemic inflammation process has been here demonstrated, adding novel information about its biological activity.

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The crude venom from the sea anemone Stichodactyla helianthus induces haemolysis and slight peroxidative damage in rat and human erythrocytes

Cited by 19 publications

References 23 publications

Haemolytic activity and characterization of nematocyst venom fromPelagia noctiluca(Cnidaria: Scyphozoa)

Haemolytic activity and characterization of nematocyst venom fromPelagia noctiluca(Cnidaria: Scyphozoa)

Marine Natural Products from the Yucatan Peninsula

Pelagia noctiluca (Scyphozoa) Crude Venom Injection Elicits Oxidative Stress and Inflammatory Response in Rats

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