The crude plant juices of desert plants as appropriate culture media for the cultivation of rhizospheric microorganisms

Nour, Eman; Hamza, M.; Fayez, M. B. E.; Monib, M.; Ruppel, Silke; Hegazi, N. A.

doi:10.1016/j.jare.2011.03.002

Cited by 25 publications

(41 citation statements)

References 31 publications

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“…Most of these studies support our finding that WPE has significant anti-fungal activity in vitro (Mohana & Raveesha, 2007;Suleiman & Ogduna, 2010;Dellavalle et al, 2011;Minz, 2012;Yenn et al, 2012). This is in contrast to other studies which have reported that WPE acted as growth promoters for endophytic fungi and bacteria (Nour et al, 2012;Osman et al, 2013).…”

Section: Discussioncontrasting

confidence: 89%

“…murinum L.) were used. These media, based on WPE of wall barley (harvested at Zadoks growth stage 07 (Zadoks, Chang, & Konzak, 1974)) were prepared following a modification to the protocol of Nour et al (2012). Briefly, whole wall barley seedlings were collected from the field in early summer before flowering shoot emergence and surface-sterilized by washing soil residues from the roots using ultra-pure water, soaking in 5% NaClO for 15 minutes, then rinsing three times in ultra-pure water.…”

Section: Preparation Of Culture Mediamentioning

confidence: 99%

“…The use of whole plant extract (WPE) as a culture medium for fungi has not been extensively studied, but at least one study has shown increased colony growth of rhizospheric micro-organisms for plant juice-based media over reference nutrient media (Nour et al, 2012). Osman et al (2013) also demonstrated that WPE from several plant species can act as growth promoters for some fungi.…”

Section: Introductionmentioning

confidence: 99%

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Media Manipulations and the Culture of Beneficial Fungal Root Endophytes

Murphy¹,

Batke²,

Doohan³

et al. 2015

IJB

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Fungal endophytes have great potential as biocontrol and biofertilisation agents for food crops, and it is important to understand and optimise the cultural conditions for endophyte emergence and growth. A range of commercial culture media were tested for their effect on endophyte recovery from the roots of their host (Hordeum murinum), and for their subsequent growth and sporulation. Furthermore, a medium composed of sterile or filtered whole plant extract (WPE) was tested at various dilutions both alone and in combination with one of the commercial media. It was found that there were significant differences between the media in endophyte recovery, endophyte mycelial growth and time to sporulation. A significantly greater number of different endophytes were recovered from roots on the malt extract plus WPE medium than any of the other media tested here. Sabouraud medium gave the greatest mean radial growth of endophyte fungal mycelia after 35 days. The cultures growing on the WPE media took over five times longer to sporulate than all other media. Results indicate that different media are suitable either for the initial recovery and isolation of fungal root endophytes or for increasing fungal biomass and inducing earlier sporulation. The experimental endophytes used here have previously been shown to have biocontrol and biofertilisation benefits for barley cultivars, so our findings have important implications for the development and commercialisation of endophyte inocula.

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Section: Discussioncontrasting

confidence: 89%

Section: Preparation Of Culture Mediamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Media Manipulations and the Culture of Beneficial Fungal Root Endophytes

Murphy¹,

Batke²,

Doohan³

et al. 2015

IJB

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“…In 2012, we proposed the use of plant-only culture media as a new approach for culturing rhizobacteria that might resist conventional cultivation. Crude plant juices, saps and powders, without any amendments, supported good growth and recoverability of rhizobacteria, comparable with the standard culture media [9–11]. For ease of application and practicability, tea bags packed with plant powders were successfully introduced to obtain the plant infusions necessary to prepare culture media [11].…”

Section: Introductionmentioning

confidence: 99%

Plant-fed versus chemicals-fed rhizobacteria of Lucerne: Plant-only teabags culture media not only increase culturability of rhizobacteria but also recover a previously uncultured Lysobacter sp., Novosphingobium sp. and Pedobacter sp.

et al. 2017

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In an effort to axenically culture the previously uncultivable populations of the rhizobacteria of Lucerne (Medicago sativa L.), we propose plant-only teabags culture media to mimic the nutritional matrix available in the rhizosphere. Here, we show that culture media prepared from Lucerne powder teabags substantially increased the cultivability of Lucerne rhizobacteria compared with a standard nutrient agar, where we found that the cultivable populations significantly increased by up to 60% of the total bacterial numbers as estimated by Quantitative Real-time Polymerase Chain Reaction (qRT-PCR). Cluster analysis of 16S rDNA Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) of cultivable Colony-Forming Units (CFUs) revealed a more distinct composition and separation of bacterial populations recovered on the plant-only teabags culture media than those developed on a standard nutrient agar. Further, the new plant medium gave preference to the micro-symbiont Sinorhizobium meliloti, and succeeded in isolating a number of not-yet-cultured bacteria, most closely matched to Novosphingobium sp., Lysobacter sp. and Pedobacter sp. The present study may encourage other researchers to consider moving from the well-established standard culture media to the challenging new plant-only culture media. Such a move may reveal previously hidden members of rhizobacteria, and help to further explore their potential environmental impacts.

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“…Our previous publications (Nour et al , Youssef et al ) provided original results and evidences on the ability of crude plant slurry homogenates, juices and saps, without any supplements, to support culturability of rhizobacteria and retrieval of their in situ populations. For ease of application and practicability, this study introduces dehydrated powders of plants ( Trifolium alexandrinum and Paspalum vaginatum ) packed in teabags to prepare liquid infusions rich enough to cultivate endo‐rhizobacteria.…”

Section: Introductionmentioning

confidence: 99%

Plant powder teabags: a novel and practical approach to resolve culturability and diversity of rhizobacteria

Sarhan

Mourad

Hamza

et al. 2016

Physiologia Plantarum

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We have developed teabags packed with dehydrated plant powders, without any supplements, for preparation of plant infusions necessary to develop media for culturing rhizobacteria. These bacteria are efficiently cultivated on such plant teabag culture media, with better progressive in situ recoverability compared to standard chemically synthetic culture media. Combining various plant-based culture media and incubation conditions enabled us to resolve unique denaturing gradient gel electrophoresis (DGGE) bands that were not resolved by tested standard culture media. Based on polymerase chain reaction PCR-DGGE of 16S rDNA fingerprints and sequencing, the plant teabag culture media supported higher diversity and significant increases in the richness of endo-rhizobacteria, namely Gammaproteobacteria (Enterobacteriaceae) and predominantly Alphaproteobacteria (Rhizobiaceae). This culminated in greater retrieval of the rhizobacteria taxa associated with the plant roots. We conclude that the plant teabag culture medium by itself, without any nutritional supplements, is sufficient and efficient for recovering and mirroring the complex and diverse communities of rhizobacteria. Our message to fellow microbial ecologists is: simply dehydrate your plant canopy, teabag it and soak it to prepare your culture media, with no need for any additional supplementary nutrients.

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The crude plant juices of desert plants as appropriate culture media for the cultivation of rhizospheric microorganisms

Cited by 25 publications

References 31 publications

Media Manipulations and the Culture of Beneficial Fungal Root Endophytes

Media Manipulations and the Culture of Beneficial Fungal Root Endophytes

Plant-fed versus chemicals-fed rhizobacteria of Lucerne: Plant-only teabags culture media not only increase culturability of rhizobacteria but also recover a previously uncultured Lysobacter sp., Novosphingobium sp. and Pedobacter sp.

Plant powder teabags: a novel and practical approach to resolve culturability and diversity of rhizobacteria

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