The CRISPR-Cas system as a tool for diagnosing and treating infectious diseases

Lou, Juan; Wang, Bin; Li, Junwei; Ni, Peng; Jin, Yuefei; Chen, Shuaiyin; Xi, Yang; Zhang, Rongguang; Duan, Guotao

doi:10.1007/s11033-022-07752-z

Cited by 19 publications

(10 citation statements)

References 101 publications

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“…At present, various pathogens can be detected with the corresponding CRISPR–Cas system, including Zika virus (Cas9); tuberculosis, human papilloma virus, HIV-1, hepatitis B, and SARS-CoV-2 viruses (Cas12); and SARS-CoV-2, Dengue, and Zika viruses (Cas13). Any of these diagnostic tests takes less than 3 h [ 62 ]. CRISPR–Cas can also be used for the construction of biosensors for detecting molecules other than nucleic acids [ 63 , 64 ].…”

Section: Crispr–cas Systems: History Of Discovery Classification and ...mentioning

confidence: 99%

Visualizing the Nucleome Using the CRISPR–Cas9 System: From in vitro to in vivo

Maloshenok

Abushinova

Ryazanova

et al. 2023

Biochemistry Moscow

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One of the latest methods in modern molecular biology is labeling genomic loci in living cells using fluorescently labeled Cas protein. The NIH Foundation has made the mapping of the 4D nucleome (the three-dimensional nucleome on a timescale) a priority in the studies aimed to improve our understanding of chromatin organization. Fluorescent methods based on CRISPR–Cas are a significant step forward in visualization of genomic loci in living cells. This approach can be used for studying epigenetics, cell cycle, cellular response to external stimuli, rearrangements during malignant cell transformation, such as chromosomal translocations or damage, as well as for genome editing. In this review, we focused on the application of CRISPR–Cas fluorescence technologies as components of multimodal imaging methods for in vivo mapping of chromosomal loci, in particular, attribution of fluorescence signal to morphological and anatomical structures in a living organism. The review discusses the approaches to the highly sensitive, high-precision labeling of CRISPR–Cas components, delivery of genetically engineered constructs into cells and tissues, and promising methods for molecular imaging.

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Section: Crispr–cas Systems: History Of Discovery Classification and ...mentioning

confidence: 99%

Visualizing the Nucleome Using the CRISPR–Cas9 System: From in vitro to in vivo

Maloshenok

Abushinova

Ryazanova

et al. 2023

Biochemistry Moscow

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“…This affordability can significantly impact the early detection and management of diseases. [152][153][154] Real-time monitoring is a crucial advantage of CRISPR technology. It allows for continuous monitoring of genetic changes in real-time, enabling timely interventions.…”

Section: Cancer Diagnosis By Crispr-cas Systemsmentioning

confidence: 99%

“…In contrast, CRISPR‐based diagnostics can be designed for cost‐effective, point‐of‐care testing, making it accessible to a broader population. This affordability can significantly impact the early detection and management of diseases [152–154] . Real‐time monitoring is a crucial advantage of CRISPR technology.…”

Section: Crispr‐cas 9 Systems: Components and Limitationsmentioning

confidence: 99%

Recent Progress in Prompt Molecular Detection of Exosomes Using CRISPR/Cas and Microfluidic‐Assisted Approaches Toward Smart Cancer Diagnosis and Analysis

Mojtaba Mousavi,

Alireza Hashemi,

Yari Kalashgrani

et al. 2023

ChemMedChem

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Exosomes are essential indicators of molecular mechanisms involved in interacting with cancer cells and the tumor environment. As nanostructures based on lipids and nucleic acids, exosomes provide a communication pathway for information transfer by transporting biomolecules from the target cell to other cells. Importantly, these extracellular vesicles are released into the bloodstream by the most invasive cells, i. e., cancer cells; in this way, they could be considered a promising specific biomarker for cancer diagnosis. In this matter, CRISPR‐Cas systems and microfluidic approaches could be considered practical tools for cancer diagnosis and understanding cancer biology. CRISPR‐Cas systems, as a genome editing approach, provide a way to inactivate or even remove a target gene from the cell without affecting intracellular mechanisms. These practical systems provide vital information about the factors involved in cancer development that could lead to more effective cancer treatment. Meanwhile, microfluidic approaches can also significantly benefit cancer research due to their proper sensitivity, high throughput, low material consumption, low cost, and advanced spatial and temporal control. Thereby, employing CRISPR‐Cas‐ and microfluidics‐based approaches toward exosome monitoring could be considered a valuable source of information for cancer therapy and diagnosis. This review assesses the recent progress in these promising diagnosis approaches toward accurate cancer therapy and in‐depth study of cancer cell behavior.

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“…(Adapted from Ref. [ 92 ] with free permission. Created with BioRender.com accessed on 21 September 2022).…”

Section: Figurementioning

confidence: 99%

Detection of Tropical Diseases Caused by Mosquitoes Using CRISPR-Based Biosensors

Zakiyyah

Ibrahim²,

Babiker³

et al. 2022

TropicalMed

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Tropical diseases (TDs) are among the leading cause of mortality and fatality globally. The emergence and reemergence of TDs continue to challenge healthcare system. Several tropical diseases such as yellow fever, tuberculosis, cholera, Ebola, HIV, rotavirus, dengue, and malaria outbreaks have led to endemics and epidemics around the world, resulting in millions of deaths. The increase in climate change, migration and urbanization, overcrowding, and other factors continue to increase the spread of TDs. More cases of TDs are recorded as a result of substandard health care systems and lack of access to clean water and food. Early diagnosis of these diseases is crucial for treatment and control. Despite the advancement and development of numerous diagnosis assays, the healthcare system is still hindered by many challenges which include low sensitivity, specificity, the need of trained pathologists, the use of chemicals and a lack of point of care (POC) diagnostic. In order to address these issues, scientists have adopted the use of CRISPR/Cas systems which are gene editing technologies that mimic bacterial immune pathways. Recent advances in CRISPR-based biotechnology have significantly expanded the development of biomolecular sensors for diagnosing diseases and understanding cellular signaling pathways. The CRISPR/Cas strategy plays an excellent role in the field of biosensors. The latest developments are evolving with the specific use of CRISPR, which aims for a fast and accurate sensor system. Thus, the aim of this review is to provide concise knowledge on TDs associated with mosquitoes in terms of pathology and epidemiology as well as background knowledge on CRISPR in prokaryotes and eukaryotes. Moreover, the study overviews the application of the CRISPR/Cas system for detection of TDs associated with mosquitoes.

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The CRISPR-Cas system as a tool for diagnosing and treating infectious diseases

Cited by 19 publications

References 101 publications

Visualizing the Nucleome Using the CRISPR–Cas9 System: From in vitro to in vivo

Visualizing the Nucleome Using the CRISPR–Cas9 System: From in vitro to in vivo

Recent Progress in Prompt Molecular Detection of Exosomes Using CRISPR/Cas and Microfluidic‐Assisted Approaches Toward Smart Cancer Diagnosis and Analysis

Detection of Tropical Diseases Caused by Mosquitoes Using CRISPR-Based Biosensors

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