The crayfish model (Orconectes rusticus), epigenetics and drug addiction research

Imeh-Nathaniel, Adebobola; Orfanakos, Vasiliki Bessie; Wormack, Leah; Huber, Robert; Nathaniel, Thomas I.

doi:10.1016/j.pbb.2019.06.003

Cited by 5 publications

(3 citation statements)

References 108 publications

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“…Invertebrates such as Caenorhabditis elegans (nematodes) and Drosophila melanogaster (fruit/vinegar flies) are valuable research models for investigating the roles of individual genes and proteins in neurotransmission, particularly those associated with alcohol-induced behaviors and neuroplasticity [30,31]. Other invertebrate models such as Schmidtea mediterranea, Giradia tigrina (planaria), Apis mellifera (honey bees), and Orconectes rusticus (crayfish) have also been used as promising models to study alcohol-related behaviors; however, they have been studied more sporadically and with lesser molecular and mechanistic amenability [32][33][34]. Therefore, this review will focus on the studies in Drosophila and C. elegans.…”

Section: Invertebrates As a Model System To Study Audmentioning

confidence: 99%

Synaptic Mechanisms of Ethanol Tolerance and Neuroplasticity: Insights from Invertebrate Models

Bhandari,

Seguin,

Rothenfluh

2024

IJMS

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Alcohol tolerance is a neuroadaptive response that leads to a reduction in the effects of alcohol caused by previous exposure. Tolerance plays a critical role in the development of alcohol use disorder (AUD) because it leads to the escalation of drinking and dependence. Understanding the molecular mechanisms underlying alcohol tolerance is therefore important for the development of effective therapeutics and for understanding addiction in general. This review explores the molecular basis of alcohol tolerance in invertebrate models, Drosophila and C. elegans, focusing on synaptic transmission. Both organisms exhibit biphasic responses to ethanol and develop tolerance similar to that of mammals. Furthermore, the availability of several genetic tools makes them a great candidate to study the molecular basis of ethanol response. Studies in invertebrate models show that tolerance involves conserved changes in the neurotransmitter systems, ion channels, and synaptic proteins. These neuroadaptive changes lead to a change in neuronal excitability, most likely to compensate for the enhanced inhibition by ethanol.

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Section: Invertebrates As a Model System To Study Audmentioning

confidence: 99%

Synaptic Mechanisms of Ethanol Tolerance and Neuroplasticity: Insights from Invertebrate Models

Bhandari,

Seguin,

Rothenfluh

2024

IJMS

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“…Cray sh are freshwater crustaceans that have become increasingly popular in epigenetic, microbiome, stem cell and evolutionary biology research. 14,19,102,103,110,114,37,40,42,82,87,94,100,101 This is primarily due to Procambarus spp small size, high fecundity, and overall environmental and nutritional adaptability. 40,78,82,101 Additionally, there is clear potential for cray sh to become a mainstay neuroregenerative and immunologic animal model.…”

Section: Introductionmentioning

confidence: 99%

Housing, Husbandry, and Clinical Techniques for Crayfish (Procambarus clarkii) used in Biomedical Research

Palillo¹,

Palillo²,

Glon³

et al. 2021

Preprint

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Crayfish (Decapoda: Astacoidea & Parastacoidea), are amongst the few animals that have stem cells within hemolymph, with the capacity to continuously produce differentiated neuronal structures throughout life. As crayfish and other invertebrates continue to become common models in research to study human disease, it is vital that we develop universal laboratory standards and guidelines on housing and husbandry practices. This publication presents introductory data on housing, husbandry, hemolymph collection and statistically supported anesthesia trials, to support future research endeavors. Evaluation of housing, husbandry, clinical and anesthetic techniques in Procambarus clarkii maintained in a biomedical research setting were performed. An option for hemolymph collection, in the area termed, the Ventral Coelomic Hemolymph Collection Zone (VCHCZ) is presented as a technique to assess hemocytes. Additionally, Wright Giemsa stained slides of crayfish hemolymph were evaluated by a board-certified veterinary clinical pathologist for interpretation and confirmation of hemolymph collection. The housing and husbandry experiments were performed over a duration of 37 days. Mortality rates and physical health assessments were performed. The following water quality parameters were concurrently evaluated: temperature, light cycle, pH, KH, GH, conductivity, total dissolved solids, salinity, ammonia, nitrite, and nitrate. Anesthetic techniques were evaluated between four experimental groups: (A) immersion in buffered MS-222 (50 mg/L), (B) immersion in buffered MS-222 (150 mg/L) (C) immersion in Propofol (65 mg/L) and (D) Propofol injection (100 mg/kg) into VCHCZ. Housing and husbandry techniques were validated with 0% mortality and normal species-specific behaviors were observed. MS-222 immersion had no observable effect on crayfish. Propofol immersion (65 mg/L) created sedative effects allowing for appropriate handling. Propofol injection (100 mg/kg) into VCHCZ successfully created a deep anesthetic plane that would allow for more invasive or surgical procedures, without adverse effects during or after recovery.

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“…Complementing rodent models, some aquatic vertebrate (e.g., zebrafish, Danio rerio), and invertebrate (e.g., crayfish) models have recently emerged to improve our understanding of emotional-like states under normal and pathological conditions (Fossat, Bacqué-Cazenave, De Deurwaerdère, Cattaert, & Delbecque, 2015;Maximino et al, 2015). Studies focusing on these models reveal some comparative relevance to humans (Fossat et al, 2015;Fossat, Bacqué-Cazenave, De Deurwaerdère, Delbecque, & Cattaert, 2014;Gerlai, 2014;Stewart, Braubach, Spitsbergen, Gerlai, & Kalueff, 2014), with the advantages of having a shorter generational time that potentially enhances the detection of developmental (e.g., drug/toxin) and transgenerational (e.g., genetic and epigenetic) effects (Imeh-Nathaniel, Orfanakos, Wormack, Huber, & Nathaniel, 2019;Lakstygal, de Abreu, & Kalueff, 2018;Scholtz et al, 2003;Spence, Gerlach, Lawrence, & Smith, 2008). Here, we call for a wider use of aquatic organisms, such as zebrafish and crayfish, for modeling stress, anxiety, aggressiveness and social preference deficits.…”

Section: Introductionmentioning

confidence: 99%

Emotional behavior in aquatic organisms? Lessons from crayfish and zebrafish

Abreu

Maximino

Banha

et al. 2019

J of Neuroscience Research

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Experimental animal models are a valuable tool to study the neurobiology of emotional behavior and mechanisms underlying human affective disorders. Mounting evidence suggests that various aquatic organisms, including both vertebrate (e.g., zebrafish) and invertebrate (e.g., crayfish) species, may be relevant to study animal emotional response and its deficits. Ideally, model organisms of disease should possess considerable genetic and physiological homology to mammals, display robust behavioral and physiological responses to stress, and should be sensitive to a wide range of drugs known to modulate stress and affective behaviors. Here, we summarize recent findings in the field of zebrafish‐ and crayfish‐based tests of stress, anxiety, aggressiveness and social preference, and discuss further perspectives of using these novel model organisms in translational biological psychiatry. Outlining the remaining questions in this field, we also emphasize the need in further development and a wider use of crayfish and zebrafish models to study the pathogenesis of affective disorders.

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The crayfish model (Orconectes rusticus), epigenetics and drug addiction research

Cited by 5 publications

References 108 publications

Synaptic Mechanisms of Ethanol Tolerance and Neuroplasticity: Insights from Invertebrate Models

Synaptic Mechanisms of Ethanol Tolerance and Neuroplasticity: Insights from Invertebrate Models

Housing, Husbandry, and Clinical Techniques for Crayfish (Procambarus clarkii) used in Biomedical Research

Emotional behavior in aquatic organisms? Lessons from crayfish and zebrafish

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