The Course of Selected Bone Resorption Marker Concentrations in Response to Short‐term Hypocalcemia Experimentally Induced with Disodium EDTA Infusions in Dairy Cows

Liesegang, Annette; Eicher, R; Sassi, Mirja-Liisa; Risteli, Juha; Riond, J.‐L.; Wanner, M.

doi:10.1046/j.1439-0442.2000.00308.x

Cited by 10 publications

(12 citation statements)

References 32 publications

(42 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To our knowledge, this delay has not been directly observed in cattle; however, we have observed indirect indications of this delay by monitoring urinary Ca in cows, which suggest that the inactivation of gastrointestinal Ca absorption may present a 2 d delay ( 29 , 32 ) . Furthermore, calcitriol sustains bone Ca mobilisation, offering an extensive pool for sustaining Ca homeostasis during lactation ( 33 ) ; however, in the short term, the readily available bone Ca is limited ( 34 ) . Calcitriol only sustains bone mobilisation if the PTH signal is maintained.…”

Section: Calcium Homeostasismentioning

confidence: 99%

A novel model to explain dietary factors affecting hypocalcaemia in dairy cattle

Martín-Tereso

Verstegen

2011

Nutr. Res. Rev.

View full text Add to dashboard Cite

Most dairy cows exhibit different degrees of hypocalcaemia around calving because the gestational Ca requirements shift to the disproportionately high Ca requirements of lactation. Ca homeostasis is a robust system that effectively adapts to changes in Ca demand or supply. However, these adaptations often are not rapid enough to avoid hypocalcaemia. A delay in the reconfiguration of intestinal Ca absorption and bone resorption is probably the underlying cause of this transient hypocalcaemia. Several dietary factors that affect different aspects of Ca metabolism are known to reduce the incidence of milk fever. The present review describes the interactions between nutrition and Ca homeostasis using observations from cattle and extrapolations from other species and aims to quantitatively model the effects of the nutritional approaches that are used to induce dry cows into an early adaptation of Ca metabolism. The present model suggests that reducing dietary cation -anion difference (DCAD) increases Ca clearance from the blood by dietary induction of systemic acidosis, which results in hypercalciuria due to the loss of function of the renal Ca transient receptor potential vanilloid channel TRPV5. Alternatively, reducing the gastrointestinal availability of Ca by reducing dietary Ca or its nutritional availability will also induce the activation of Ca metabolism to compensate for basal blood Ca clearance. Our model of gastrointestinal Ca availability as well as blood Ca clearance in the transition dairy cow allowed us to conclude that the most common dietary strategies for milk fever prevention may have analogous modes of action that are based on the principle of metabolic adaptation before calving.

show abstract

Section: Calcium Homeostasismentioning

confidence: 99%

A novel model to explain dietary factors affecting hypocalcaemia in dairy cattle

Martín-Tereso

Verstegen

2011

Nutr. Res. Rev.

View full text Add to dashboard Cite

show abstract

“…In previous studies investigating mineral metabolism, administration of Na 2 EDTA was mainly used to induce the hypocalcemic status in cattle [ 5 , 14 ]. Several studies that induced hypocalcemia by EDTA infusion have identified changes in Ca absorption in the intestine, resorption of Ca from bone and immune suppression [ 8 , 14 , 20 ]. In addition, the change in blood Ca concentration and other changes such as a liver enzyme, hormones and electrolytes were confirmed [ 5 , 14 , 17 ].…”

mentioning

confidence: 99%

“…Several studies that induced hypocalcemia by EDTA infusion have identified changes in Ca absorption in the intestine, resorption of Ca from bone and immune suppression [ 8 , 14 , 20 ]. In addition, the change in blood Ca concentration and other changes such as a liver enzyme, hormones and electrolytes were confirmed [ 5 , 14 , 17 ]. On the other hand, in some previous studies [ 5 , 14 , 20 ], normal saline and CaEDTA solution were considered as solutions to be infused to the control group, and we used normal saline for administration to CON.…”

mentioning

confidence: 99%

“…In addition, the change in blood Ca concentration and other changes such as a liver enzyme, hormones and electrolytes were confirmed [ 5 , 14 , 17 ]. On the other hand, in some previous studies [ 5 , 14 , 20 ], normal saline and CaEDTA solution were considered as solutions to be infused to the control group, and we used normal saline for administration to CON. Because the purpose of this study was to identify changes in blood parameters, especially pH, in hypocalcemia and normal status, normal saline was used to minimize other physiological changes.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Changes in plasma pH and blood and urinary macromineral concentrations in experimentally induced hypocalcemic cows with Na<sub>2</sub>EDTA

Choi

Park

et al. 2020

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

This study was performed to confirm the alterations of blood and urine parameters in artificially induced hypocalcemic cows. For a 2 × 2 cross-over design, four non-pregnant, non-lactating Holstein Friesian cows (623 ± 63 kg) were utilized. Cows in the treatment and control group were infused with ethylenediaminetetraacetic acid (Na 2 EDTA) solution and normal saline through an intravenous catheter for 3 hr, respectively. Laboratory analyses included complete blood cell count, plasma chemistry, blood gas analysis and urine chemistry. During the hypocalcemic period, abnormal signs were not observed clinically, hematologically nor biochemically either in groups. But, plasma calcium and magnesium concentrations continued to decrease throughout Na 2 EDTA infusion, and significant group differences ( P <0.05 or P <0.001) were detected until 5 hr after the initiation of infusion. Urinary excretions of these minerals were significantly reduced compared to the control group by 6 hr (Ca, P <0.05; Mg, P <0.001). Moreover, there is a significant group difference in the change in plasma pH at 1 hr after Na 2 EDTA infusion ( P <0.05) and maintained a decreased level until 6 hr. Consequently, the blood pH was diminished simultaneously with hypocalcemia and hypomagnesemia induction in cows infused with Na 2 EDTA. This phenomenon may be one of the mechanisms to recover normocalcemia including maximizing the effect of parathyroid hormone, however, further studies are needed to elucidate the mechanism to alter the blood pH in hypocalcemia.

show abstract

“…Serum was also analysed for osteocalcin, a bone formation marker, and pyridinoline, a bone resorption maker, using ELISA (Lester, 1995; Liesegang et al., 2000a). Additionally, the first serum sample obtained after parturition was analysed for parathyroid hormone (Lester, 1995; Liesegang et al., 2000b) and 1,25‐dihydroxy vitamin D (RIA kit; DiaSorin, Stillwater, MN, USA).…”

Section: Methodsmentioning

confidence: 99%

Body phosphorus mobilization and deposition during lactation in dairy cows

Elizondo-Salazar

Ferguson

Beegle

et al. 2012

Animal Physiology Nutrition

View full text Add to dashboard Cite

Dairy cow bone phosphorus (P) mobilization and deposition and their influence on P requirements were studied over the lactation cycle. Thirty Holsteins received a common diet during the dry period and one of the following three dietary treatments that varied in P percentage during the subsequent lactation (44 weeks): (i) 0.36 throughout (constant P, 0.36-0.36-0.36), (ii) 0.36 for 30 weeks then 0.29 for 14 weeks (P changed once, 0.36-0.36-0.29), and (iii) 0.43 for 10 weeks, 0.36 for 20 weeks, and 0.29 for 14 weeks (P changed twice, 0.43-0.36-0.29). Six P balance studies were conducted during the experiment, including one during the dry period and five along lactation, based on P intake, faecal P, urinary P and milk P, when appropriate. Blood samples were taken during balance to analyse bone formation (osteocalcin) and resorption (pyridinoline) marker concentrations and rib biopsies performed to determine bone P content. Phosphorus balance was negative during weeks -4 to -1 relative to lactation for all groups and remained negative for cows fed 0.36% P during weeks 1-5, but showed a positive value for cows that received 0.43% P. The balance was close to zero for all groups at weeks 19-23 and showed a clear retention during weeks 38-42; by the end of lactation, cows re-stored most of the P mobilized earlier. The pattern in P balance was consistent with changes in blood bone metabolism marker concentrations, rib bone P content, and faecal and urinary P concentrations over the experiment, indicating that cows, irrespective of the dietary P treatments received, mobilized P from bone during the late dry period when fed a low-Ca diet and early lactation, and re-stored P in late lactation. This dynamic of P metabolism can have important implications for dietary P requirements and ration formulations.

show abstract

The Course of Selected Bone Resorption Marker Concentrations in Response to Short‐term Hypocalcemia Experimentally Induced with Disodium EDTA Infusions in Dairy Cows

Cited by 10 publications

References 32 publications

A novel model to explain dietary factors affecting hypocalcaemia in dairy cattle

A novel model to explain dietary factors affecting hypocalcaemia in dairy cattle

Changes in plasma pH and blood and urinary macromineral concentrations in experimentally induced hypocalcemic cows with Na<sub>2</sub>EDTA

Body phosphorus mobilization and deposition during lactation in dairy cows

Contact Info

Product

Resources

About