Abstract:BackgroundPlantlets grown in vitro with a mixed nitrogen source utilize sucrose and CO2 as carbon sources for growth. However, it is very difficult to obtain the correct proportion of assimilated nitrate, ammonium, sucrose and CO2 for plantlets. Consequently, the NH4+/NO3- use efficiency for carbon fixation derived from the assimilation of sucrose/CO2 is still unclear for plantlets. ResultsThe bidirectional stable nitrogen isotope tracer technique was employed to quantify the proportions of nitrate and ammoniu… Show more
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