The cotton MYB108 forms a positive feedback regulation loop with CML11 and participates in the defense response against<i>Verticillium dahliae</i>infection

Cheng, Huanqing; Han, Libo; Yang, Chun-Lin; Wu, Xiaomin; Zhong, Nanshan; Wu, Jiahe; Wang, Fuxin; Wang, Haiyun; Xia, Gui-Xian

doi:10.1093/jxb/erw016

Cited by 96 publications

(61 citation statements)

References 58 publications

(67 reference statements)

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“…3). MYB108 has been implicated in jasmonate-mediated stamen maturation in Arabidopsis (Mandaokar & Browse, 2009), abscisic acid-induced cell death (Cui et al, 2013) and in pathogen defence in cotton (Cheng et al, 2016). Homologues of AtMYB78 have been described as playing a role during response to heat and drought stress in soybean and sorghum (Pereira et al, 2011;Johnson et al, 2014).…”

Section: Researchmentioning

confidence: 99%

A kiwifruit (Actinidia deliciosa) R2R3‐MYB transcription factor modulates chlorophyll and carotenoid accumulation

et al. 2018

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MYB transcription factors (TFs) regulate diverse plant developmental processes and understanding their roles in controlling pigment accumulation in fruit is important for developing new cultivars. In this study, we characterised kiwifruit TFMYB7, which was found to activate the promoter of the kiwifruit lycopene beta-cyclase (AdLCY-β) gene that plays a key role in the carotenoid biosynthetic pathway. To determine the role of MYB7, we analysed gene expression and metabolite profiles in Actinidia fruit which show different pigment profiles. The impact of MYB7 on metabolic biosynthetic pathways was then evaluated by overexpression in Nicotiana benthamiana followed by metabolite and gene expression analysis of the transformants. MYB7 was expressed in fruit that accumulated carotenoid and Chl pigments with high transcript levels associated with both pigments. Constitutive over-expression of MYB7, through transient or stable transformation of N. benthamiana, altered Chl and carotenoid pigment levels. MYB7 overexpression was associated with transcriptional activation of certain key genes involved in carotenoid biosynthesis, Chl biosynthesis, and other processes such as chloroplast and thylakoid membrane organization. Our results suggest that MYB7 plays a role in modulating carotenoid and Chl pigment accumulation in tissues through transcriptional activation of metabolic pathway genes.

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Section: Researchmentioning

confidence: 99%

A kiwifruit (Actinidia deliciosa) R2R3‐MYB transcription factor modulates chlorophyll and carotenoid accumulation

et al. 2018

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“…We also found several MYB and WRKY transcription factor homologues up-regulated at 2 h and 8 days post-inoculation, respectively. For instance, MYB108 and MYB44 homologues, which were up-regulated in AS fruit, have been related to the enhanced tolerance to Verticillium dahliae Klebahn in cotton and to the resistance to Penicillium simplicissimum (Oudemans) Thom in Arabidopsis (Cheng et al, 2016;Hieno et al, 2016). A WRKY40 homologue, which was highly up-regulated at 8 days after inoculation, has been proposed recently as a negative regulator of cell death (Lee et al, 2017), thus potentially explaining, at least in part, the absence of hard necrotic lesions in AS fruit.…”

Section: Discussionmentioning

confidence: 99%

Engineering d‐limonene synthase down‐regulation in orange fruit induces resistance against the fungus Phyllosticta citricarpa through enhanced accumulation of monoterpene alcohols and activation of defence

Rodríguez

Kava

Latorre-García

et al. 2018

Molecular Plant Pathology

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Terpene volatiles play an important role in the interactions between specialized pathogens and fruits. Citrus black spot (CBS), caused by the fungus Phyllosticta citricarpa, is associated with crop losses in different citrus-growing areas worldwide. The pathogen may infect the fruit for 20-24 weeks after petal fall, but the typical hard spot symptoms appear when the fruit have almost reached maturity, caused by fungal colonization and the induction of cell lysis around essential oil cavities. d-Limonene represents approximately 95% of the total oil gland content in mature orange fruit. Herein, we investigated whether orange fruit with reduced d-limonene content in peel oil glands via an antisense (AS) approach may affect fruit interaction with P. citricarpa relative to empty vector (EV) controls. AS fruit showed enhanced resistance to the fungus relative to EV fruit. Because of the reduced d-limonene content, an over-accumulation of linalool and other monoterpene alcohols was found in AS relative to EV fruit. A global gene expression analysis at 2 h and 8 days after inoculation with P. citricarpa revealed the activation of defence responses in AS fruit via the up-regulation of different pathogenesis-related (PR) protein genes, probably as a result of enhanced constitutive accumulation of linalool and other alcohols. When assayed in vitro and in vivo, monoterpene alcohols at the concentrations present in AS fruit showed strong antifungal activity. We show here that terpene engineering in fruit peels could be a promising method for the development of new strategies to obtain resistance to fruit diseases.

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“…In cotton plants, GhWRKY40 has been reported to 299 regulate wounding and resistance response against Among the 34 down-regulated MYB TFs (S4 Table), we found three genes encoding for 312 MYB108 at 3 and 7 dpi. Arabidopsis AtMYB108 has been characterized as an important 313 regulator of both biotic and abiotic stresses (Mengiste et al, 2003) It is also known that the 314 expression of GhMYB108 in cotton, responds to application of defense-related phytohormones 315 such as SA, JA and ET (Cheng et al, 2016). The absence of GhMYB108 led to increased 316 susceptibility of cotton plants to Verticillium dahliae infection while its ectopic overexpression 317 enhanced tolerance to the fungal pathogen (Cheng et al, 2016).…”

Section: Transcriptome Data Analysis and Functional Annotation Of Difmentioning

confidence: 99%

“…Arabidopsis AtMYB108 has been characterized as an important 313 regulator of both biotic and abiotic stresses (Mengiste et al, 2003) It is also known that the 314 expression of GhMYB108 in cotton, responds to application of defense-related phytohormones 315 such as SA, JA and ET (Cheng et al, 2016). The absence of GhMYB108 led to increased 316 susceptibility of cotton plants to Verticillium dahliae infection while its ectopic overexpression 317 enhanced tolerance to the fungal pathogen (Cheng et al, 2016). This would, therefore, indicate 318 that down-regulation of MYB108 coding genes interfered with the defense signaling pathway 319 leading to a compatible response.…”

Section: Transcriptome Data Analysis and Functional Annotation Of Difmentioning

confidence: 99%

Transcriptional profiling of potato (Solanum tuberosum L.) during a compatible interaction with the root-knot nematode, Meloidogyne javanica

Macharia

Bellieny-Rabelo

Moleleki

2019

Preprint

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Transcriptional profiling of potato (Solanum tuberosum L.) during a compatible interaction with the 1 root-knot nematode, Meloidogyne javanica 2 3Running title: "" Response of potato (Solanum tuberosum L.) to root-knot nematode, 4 Meloidogyne javanica 5 6Abstract 14Root-knot nematode (RKN, Meloidogyne javanica) presents a great challenge to Solanaceae 15 crops, including the potato. In this report, we conducted an investigation to understand the 16 transcriptional regulation of molecular responses in potato roots during a compatible 17 interaction following RKN infection. In this study, analysis of gene expression profiles using 18 RNA-seq of Solanum tuberosum cv Mondial with RKN interaction at 0, 3-and 7-days post-19 inoculation (dpi). In total, 4,948 and 4,484 genes were respectively detected as differentially 20 expressed genes (DEGs) at 3 and 7 dpi. Functional annotation revealed that genes associated 21 with metabolic process were enriched at the transcriptional level suggesting they have an 22 important role in RKN disease development. Nematode infection caused down-regulation of 23 282 genes associated with pathogen perception hence interfering with activation plant immune 24 system. Further, late activation of pathogenesis-related genes, down-regulation disease 25 resistance genes and activation of host antioxidant system contributed to a susceptible response. 26 Activation of Jasmonic acid (JA) pathway and protease inhibitors was due to wounding during 27 nematode migration and feeding. Nematode infection suppressed ethylene (ET) and salicylic 28 acid (SA) signalling pathway hindering SA/ET responsive genes involved with defense. 29 Induction of auxin biosynthesis genes, regulation of cytokinin levels and up-regulation of 30 transporter genes facilitated of nematode feeding sites (NFSs) initiation. The regulation of 31 several families of transcription factors (TFs) in the plant, such as WRKY, GRAS, ERF BHLH 32 and MYB, was affected by RKN infection disrupting plant defense signalling pathways. This 33 clearly suggest that TFs played an indispensable role in physiological adaptation for successful 34 RKN disease development. This genome-wide analysis revealed the molecular regulatory 35 networks in potato roots which are successfully manipulated by RKN. Being the first study 36analysing transcriptome profiling of RKN diseased potato, it will provide unparalleled insight 37 into the mechanism underlying disease development. 38Key words: Root-knot nematode, Solanum tuberosum, nematode feeding sites, differentially 39 expressed genes 40Meloidogyne spp are obligate and highly polyphagous pests that form an intricate relationship 46 with their host causing drastic morphological and physiological changes in plant cell 47 643

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The cotton MYB108 forms a positive feedback regulation loop with CML11 and participates in the defense response againstVerticillium dahliaeinfection

Abstract: HighlightCotton MYB108 interacts with CML11 and acts as a positive regulator in defense against V. dahliae infection.

Cited by 96 publications

References 58 publications

A kiwifruit (Actinidia deliciosa) R2R3‐MYB transcription factor modulates chlorophyll and carotenoid accumulation

A kiwifruit (Actinidia deliciosa) R2R3‐MYB transcription factor modulates chlorophyll and carotenoid accumulation

Engineering d‐limonene synthase down‐regulation in orange fruit induces resistance against the fungus Phyllosticta citricarpa through enhanced accumulation of monoterpene alcohols and activation of defence

Transcriptional profiling of potato (Solanum tuberosum L.) during a compatible interaction with the root-knot nematode, Meloidogyne javanica

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