The Correlation of Reactive Changes in the Cortical and Cerebral Substance of the Adrenal Glands of White Rats During the Correction of Testosterone Blockade at the 6th Month of the Experiment

Abstract

The main pharmacological effect of prolonged injection of a synthetic analogue of gonadotropin-releasing hormone is the development of chemical castration. To correct the reactive changes that develop during testosterone blockade in the organs under study, scientists use the active ingredient quercetin to study its effect on oxidative stress. Its antioxidant effect can be used for pathogenetic therapy and prevention of changes in the body caused by a decrease in testosterone levels [12, 13, 18]. The aim of the study was to investigate morphological changes in corticosterocytes zones of the cortex and medullary endocrinocytes of the adrenal glands of white rats during the correction of central testosterone deprivation at the sixth month of the experiment. Materials and methods: The adrenal glands of 25 male white rats were studied and divided into 3 groups: group 1 – 5 intact rats, group 2 – 10 rats injected with triptorelin acetate subcutaneously at a dose of 0.3 mg of active substance per kg of body weight, and group 3 – 10 rats injected with quercetin 100 mg per kg of body weight once a day 3 times a week for 6 months against the background of triptorelin acetate administration. The average size and volume of the nucleus (N) of corticosterocytes of all cortical zones (CZ), the average size and volume of the nucleus of the ChC, the density of the cords of the adrenal cortex zones and the density of the cords of the adrenal medulla were measured and calculated. Conclusions: 1. Correction of central testosterone deprivation at the 6th month of the experiment causes a decrease in the synthetic activity of corticosterocytes of the glomerular zone and an increase in the protein synthetic function of fascicular zone cells. 2. A significant decrease in the average size of corticosterocytes of the reticular zone and restoration of cytoplasmic basophilia at 6 months of correction proves the positive effect of quercetin on the synthetic activity of these cells. 3. Reduction of the average size and volume of chromophilic endocrinocyte nuclei and increase in their number during the correction of chemical castration at 6 months of the experiment confirms the significant positive effect of quercetin. 4. The revealed correlation between changes in the cells of the glomerular, fascicular and reticular zones of the adrenal cortex and the adrenal medulla of white rats against the background of chemical castration and its correction indicates the involvement of not only hypothalamic-pituitary dependent endocrine organs, but also organs of the sympathoadrenal system.