The correlation of pore morphology, interconnectivity and physical properties of 3D ceramic scaffolds with bone ingrowth

Jones, Anthony; Arns, Christoph H.; Hutmacher, Dietmar W.; Milthorpe, Bruce; Sheppard, Adrian; Knackstedt, Mark

doi:10.1016/j.biomaterials.2008.10.056

Cited by 308 publications

(206 citation statements)

References 37 publications

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“…[36] Scarano et al [17,32] recently found that mineralized bone was present inside a large portion of the Algipore particles, which have a mean pore diameter of about 10 μm. This fact is in contrast with other studies [18,37] that stated that pores smaller than 10 μm inhibit cellular ingrowth, while pores between 15 μm and 50 μm help fi brovascular colonization, pores between 50 μm and 150 μm determine osteoid growth, and pores larger than 150 μm facilitate internal mineralized bone formation. …”

Section: S E V E R a L S T U D I E S R E P O R T E D T H E U S I N G contrasting

confidence: 80%

Maxillary sinus floor augmentation with vegetal hydroxyapatite "versus" demineralized bovine bone: A randomized clinical study with a split-mouth design

et al. 2014

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Purpose: The objective of this paper was to compare histologically and histomorphometrically a hydroxyapatite originated by algae (Algipore) versus demineralized bovine bone (Bio-Oss Geistlich Pharma, Wolhusen, Switzerland) utilised as bone substitutes in maxillary sinus floor elevation with a split-mouth design. Materials and Methods: Five healthy patients underwent a bilateral maxillary sinus floor elevation procedure under local anesthesia. In each case, residual posterior maxillary bone height was between 2 mm and 5 mm. The original bone was augmented with a split-mouth design with 100% Algipore on the test side and 100% Bio-Oss on the contralateral control side. After a healing period of 6-8 months during the re-opening surgery biopsies were retrieved and Xive Implants (Dentsply Implants, Mannheim, Germany) were placed. Results: At microscopic level both Bio-Oss and Algipore blocks resulted well osseointegrated, without inflammatory infiltrate, with an high level of mineralization, without gap between the bone and biomaterial interfaces that resulted indistinguishable. A close contact between the two faces was observed without the presence of slits. Histomorphometrical analysis showed that, on average, the percentage of medullary space was higher for the Bio-Oss compared with Algipore (38.61% ±8.90% vs. 29.23% ±7.89%). In contrast, the mean value of residual particles of biomaterials was higher in Algipore than in Bio-Oss specimens (42.86% ±18.61% vs. 22.30% ±6.40% respectively). Conclusions: The data confirmed that sinus lift carried out with Algipore performed in a similar way of that carried out with Bio-Oss and that this material is safe, predictable and without invasiveness.

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Section: S E V E R a L S T U D I E S R E P O R T E D T H E U S I N G contrasting

confidence: 80%

Maxillary sinus floor augmentation with vegetal hydroxyapatite "versus" demineralized bovine bone: A randomized clinical study with a split-mouth design

et al. 2014

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“…20 The low and high permeability designs have porosities of 53.46% and 70%, and surface areas of 317.69 and 260.52 mm 2 , respectively. The designs and associated properties are displayed in Table 1.…”

Section: Scaffold Fabricationmentioning

confidence: 99%

“…1,2 Unlike porosity, pore size, and a number of other structural parameters that have been studied, permeability defines the physical property of mass transport, which inherently describes the effects that these structural design properties have on fluid transport into and out of a construct. The effects that scaffold permeability has on bone tissue regeneration have not been studied in depth using rigorously controlled porous architectures with reproducibly designed effective permeability.…”

mentioning

confidence: 99%

Effect of Polycaprolactone Scaffold Permeability on Bone Regeneration In Vivo

Mitsak

Kemppainen

Harris

et al. 2011

Tissue Engineering Part A

155

114

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Successful bone tissue engineering depends on the scaffold's ability to allow nutrient diffusion to and waste removal from the regeneration site, as well as provide an appropriate mechanical environment. Since bone is highly vascularized, scaffolds that provide greater mass transport may support increased bone regeneration. Permeability encompasses the salient features of three-dimensional porous scaffold architecture effects on scaffold mass transport. We hypothesized that higher permeability scaffolds will enhance bone regeneration for a given cell seeding density. We manufactured poly-e-caprolactone scaffolds, designed to have the same internal pore design and either a low permeability (0.688 · 10 /N-s), respectively. Scaffolds were seeded with bone morphogenic protein-7-transduced human gingival fibroblasts and implanted subcutaneously in immune-compromised mice for 4 and 8 weeks. Micro-CT evaluation showed better bone penetration into high permeability scaffolds, with blood vessel infiltration visible at 4 weeks. Compression testing showed that scaffold design had more influence on elastic modulus than time point did and that bone tissue infiltration increased the mechanical properties of the high permeability scaffolds at 8 weeks. These results suggest that for polycaprolactone, a more permeable scaffold with regular architecture is best for in vivo bone regeneration. This finding is an important step toward the end goal of optimizing a scaffold for bone tissue engineering.

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“…In particular, Xray microfocus computed tomography (micro-CT) has been frequently applied as a 3D quantitative imaging technique to assess the scaffold structure, [6][7][8] as well as bone ingrowth after in vivo implantation. 6,[9][10][11][12][13][14] Furthermore, it has been employed for time-lapsed follow-up of mineralization inside scaffolds during in vitro static [15][16][17] or bioreactor cultures. 13,18,19 In most of these studies, polymeric, ceramic, collagen scaffolds, or composites were used, in which, the mineralized extracellular matrix (ECM) could be separated from the scaffold for the purpose of volume calculations and no significant material-dependent artifacts were present.…”

Section: Introductionmentioning

confidence: 99%

Three-Dimensional Characterization of Tissue-Engineered Constructs by Contrast-Enhanced Nanofocus Computed Tomography

Papantoniou

Sonnaert

Geris

et al. 2014

Tissue Engineering Part C: Methods

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To successfully implement tissue-engineered (TE) constructs as part of a clinical therapy, it is necessary to develop quality control tools that will ensure accurate and consistent TE construct release specifications. Hence, advanced methods to monitor TE construct properties need to be further developed. In this study, we showed proof of concept for contrast-enhanced nanofocus computed tomography (CE-nano-CT) as a whole-construct imaging technique with a noninvasive potential that enables three-dimensional (3D) visualization and quantification of in vitro engineered extracellular matrix (ECM) in TE constructs. In particular, we performed a 3D qualitative and quantitative structural and spatial assessment of the in vitro engineered ECM, formed during static and perfusion bioreactor cell culture in 3D TE scaffolds, using two contrast agents, namely, Hexabrix Ò and phosphotungstic acid (PTA). To evaluate the potential of CE-nano-CT, a comparison was made to standardly used techniques such as Live/Dead viability/cytotoxicity, Picrosirius Red staining, and to net dry weight measurements of the TE constructs. When using Hexabrix as the contrast agent, the ECM volume fitted linearly with the net dry ECM weight independent from the flow rate used, thus suggesting that it stains most of the ECM. When using PTA as the contrast agent, comparing to net weight measurements showed that PTA only stains a part of the ECM. This was attributed to the binding specificity of this contrast agent. In addition, the PTA-stained CE-nano-CT data showed pronounced distinction between flow conditions when compared to Hexabrix, indicating culture-specific structural ECM differences. This novel type of information can contribute to optimize bioreactor culture conditions and potentially critical quality characteristics of TE constructs such as ECM quantity and homogeneity, facilitating the gradual transformation of TE constructs in well-characterized TE products.

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The correlation of pore morphology, interconnectivity and physical properties of 3D ceramic scaffolds with bone ingrowth

Cited by 308 publications

References 37 publications

Maxillary sinus floor augmentation with vegetal hydroxyapatite "versus" demineralized bovine bone: A randomized clinical study with a split-mouth design

Maxillary sinus floor augmentation with vegetal hydroxyapatite "versus" demineralized bovine bone: A randomized clinical study with a split-mouth design

Effect of Polycaprolactone Scaffold Permeability on Bone Regeneration In Vivo

Three-Dimensional Characterization of Tissue-Engineered Constructs by Contrast-Enhanced Nanofocus Computed Tomography

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